2011
DOI: 10.1186/1477-3155-9-57
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Efficiency, error and yield in light-directed maskless synthesis of DNA microarrays

Abstract: BackgroundLight-directed in situ synthesis of DNA microarrays using computer-controlled projection from a digital micromirror device--maskless array synthesis (MAS)--has proved to be successful at both commercial and laboratory scales. The chemical synthetic cycle in MAS is quite similar to that of conventional solid-phase synthesis of oligonucleotides, but the complexity of microarrays and unique synthesis kinetics on the glass substrate require a careful tuning of parameters and unique modifications to the s… Show more

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Cited by 60 publications
(98 citation statements)
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“…MicroRNA micorarrays were synthesized using maskless microarray synthesis as described by Agbavwe et al [2011], with a modified reaction chamber allowing for the simultaneous synthesis of microarrays on two independent glass substrates as described by Sack et al [2013]. The customized microarray comprises probes for all known mature mouse miRNA sequences from release 19 of miRBase [Griffiths‐Jones et al, 2006, 2008; Kozomara and Griffiths‐Jones, 2011], as described previously by Rohm et al [2015].…”
Section: Methodsmentioning
confidence: 99%
“…MicroRNA micorarrays were synthesized using maskless microarray synthesis as described by Agbavwe et al [2011], with a modified reaction chamber allowing for the simultaneous synthesis of microarrays on two independent glass substrates as described by Sack et al [2013]. The customized microarray comprises probes for all known mature mouse miRNA sequences from release 19 of miRBase [Griffiths‐Jones et al, 2006, 2008; Kozomara and Griffiths‐Jones, 2011], as described previously by Rohm et al [2015].…”
Section: Methodsmentioning
confidence: 99%
“…5,6) Then, maskless lithography appeared, where patterns were created by light reflection either from analog or digital mirrors. [7][8][9][10] Direct synthesis of DNA on a platform is important because today real-time PCR and DNA microarrays are the most commonly used molecular diagnostics tools for in-vitro diagnosis (IVD). With the real-time PCR, one can determine the amount of viral particles in blood and quantitatively analyze the number of DNA in samples.…”
Section: Introductionmentioning
confidence: 99%
“…This would reduce the sequence fidelity and the ability to miniaturize the arrays. [11] By contrast, the array in [C] shows no evidence of substrate degradation and the spatial separation between features is preserved. [C] was synthesized upon ammonia-treated PET using iodine/pyridine/water in THF as an oxidizer and included a step of acetylation to cap failed sequences during synthesis.…”
mentioning
confidence: 99%