Efficiency of Antimicrobial Electrospun Thymol-Loaded Polycaprolactone Mats In Vivo

Abstract

Due to the prevalence of antimicrobial resistant pathogens, natural products with long-term antimicrobial activities are considered as potential alternatives. In this work, polycaprolactone (PCL) electrospun fibers with mean diameters around 299 nm and loaded with 14.92 ± 1.31% w/w thymol (THY) were synthesized. The mats had appropriate elongation at break (74.4 ± 9.5%) and tensile strength (3.0 ± 0.5 MPa) to be potentially used as wound dressing materials. In vivo studies were performed using eight to ten week-old male SKH1 hairless mice. The infection progression was evaluated through a semiquantitative method and quantitative polymerase chain reaction. The analyses of postmortem samples indicated that THY-loaded PCL fibers acted as inhibitors of Staphylococcus aureus ATCC 25923 strain growth being as efficient as chlorhexidine (CLXD). Histopathological and immunohistochemical studies showed that the PCL-THY-treated wounds were almost free of an inflammatory reaction. Therefore, wound dressings containing natural compounds can prevent infection and promote wound healing and prompt regeneration.

Unraveling the Adsorption Behavior of Thymol on Carbon and Silica Nanospheres for Prolonged Antibacterial Activity: Experimental and DFT Studies

“…aureus and were comparable to carbon-based materials reported elsewhere. ,, In addition, all of the results showed promising beneficial materials for wound dressing applications. However, fabrication processes as well as in vivo assessments of biocompatibility are still needed. − Therefore, carbon nanospheres should be further developed and utilized in preventive healthcare and medical devices, e.g., as drug nanocarriers and inhibition products.…”

Probing the Effects of Antimicrobial‐Lysozyme Derivatization on Enzymatic Degradation of Poly(ε‐caprolactone) Film and Fiber

“…[42,43] It is evident from the literature that PCL has been employed for biological applications both in thin film and micro/nanostructured forms like electrospun (ES) fibers or 3D printed scaffolds. [44][45][46][47][48] While non-patterned thin films are frequently used as coatings on catheters and surgical devices, micro/nanostructuring is deemed to be crucial for modulating the local microenvironment of the implant host matrix to facilitate cell proliferation and tissue regeneration by mimicking the extracellular matrix. [49] The current work, therefore, involves fabricating PCL films and ES fibers that represent non-structured and structured scaffolds, respectively, and conferring them with antimicrobial properties by covalently immobilizing lysozyme onto the surfaces.…”

Exosomes loaded with ultrasmall Pt nanoparticles: a novel low-toxicity alternative to cisplatin

“…Indeed, exosomes (soft extracellular vesicles produced by the cells, 50 to 150 nm in size) have significant biological content, including DNA, RNA and proteins, and thanks to the specific composition of their membranes, are able to target desired organs[ 7 – 10 ] giving them great potential as selective delivery vectors. Theragnostic nanomaterials have been also incorporated in these vesicles especially in applications against cancer [ 8 , 9 , 11 – 27 ]. In this case, exosomes provide nanomaterials with a natural and robust delivery vehicle with high blood circulation time, low immunogenicity and ability to target specific cells or tissues [ 28 , 29 ].…”