Efficiency of durum wheat seeds biopriming by rhizobacteria in the biocontrol of <i>Fusarium culmorum</i> and <i>Fusarium chlamydosporum</i> infecting durum wheat in Algeria

Bencheikh, Amor; Hicham, Meziti; Meriem, Daichi Barkahoum; Gharzouli, Asma; Khalida, Belkadi; Rouag, Noureddine

doi:10.1080/03235408.2021.2025006

Cited by 7 publications

(5 citation statements)

References 42 publications

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“…Wheat seeds were soaked in microbial suspensions ( P. plecoglossicedea NR_114226 at 10 8 CFU/mL) and dried at room temperature for 30 min. The seeds were then sown in pots containing sterilized soil previously inoculated with explants of different strains of Fusarium , at a density of four seeds per pot (Abdallah‐Nekache et al., 2019; Bencheikh et al., 2022). Untreated seeds (neither Fusarium spp.…”

Section: Methodsmentioning

confidence: 99%

Pseudomonas plecoglossicida(NR_114226) as a novel biocontrol agent against Fusarium crown rot of wheat

Makhlouf,

Karima,

Slimane

2024

Journal of Phytopathology

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Fusarium crown rot (FCR) poses a significant threat to wheat crops, causing substantial grain loss and mycotoxin contamination. While chemical fungicides have been effective in controlling this disease, the need for environmentally friendly alternatives is paramount due to concerns about human health and fungicide resistance. This study delves into the efficacy of Pseudomonas plecoglossicida (NR_114226) as a plant growth‐promoting and biocontrol agent against Fusarium graminearum (ON685926) and Fusarium pseudograminearum (ON687723). Greenhouse experiments demonstrated a significant reduction in disease severity by 66.06% through seed treatment with this rhizobacterial strain in soil already infested with fungal pathogens. Moreover, significant growth enhancement occurred in F. pseudograminearum‐infected seedlings treated with P. plecoglossicida (F4 + P57), increasing plant height from 13.8 to 33.1 cm and root length from 9.56 to 19.62 cm, with notable improvements in fresh and dry weights. The study further validated these findings through enzymatic assays and analysis of synthesized secondary metabolites, including chitinase, protease, hydrocyanic acid, indole acetic acid, phosphate solubilization and siderophore production. These results strongly suggest that P. plecoglossicida has the potential to serve as an effective biocontrol agent against soilborne cereal pathogens.

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Section: Methodsmentioning

confidence: 99%

Pseudomonas plecoglossicida(NR_114226) as a novel biocontrol agent against Fusarium crown rot of wheat

Makhlouf,

Karima,

Slimane

2024

Journal of Phytopathology

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“…The bacterial strains Bacillus amyloliquefaciens (B18) and Bacillus subtilis (S8) previously obtained from the wheat rhizosphere from Bordj Bou Arreridj and Setif province respectively, Algeria. Through macro and micro-morphological, biochemical, and molecular assessment, the bacterial strains were identified (Bencheikh et al, 2022). Bacterial strains were stored in 30 % glycerol at −20 °C.…”

Section: Antifungal Bacteriamentioning

confidence: 99%

“…FCR agent, Fusarium culmorum Using morphological and molecular characterization, the previously obtained F. culmorum (FC) isolate, which was acquired from durum wheat seeds, was identified (Bencheikh et al, 2022). The F. culmorum isolate was maintained at 25 °C on Potato Sucrose Agar (PSA).…”

Section: Antifungal Bacteriamentioning

confidence: 99%

“…Three seeds (cv. Boutaleb), surface sterilized (Bencheikh et al, 2022) and submerged in the suspension of bacterial strain for 3h, were displayed in each pot. Each seed received one 6 mm diameter plug from a 7-day-old F. culmorum culture.…”

Section: Growth Chamber Experimentsmentioning

confidence: 99%

“…These latter have shown an interesting antifungal activity each alone or in combination compared to tebuconazole in the in vitro control of two strains of Venturia inaequalis, the responsible agent of apple scab (Desmyttere et al, 2019). Previous research revealed that the B. subtilis S8 strain and B. amyloliquefaciens B18 were potential agents in biocontrol of F. culmorum isolates (FC1 and FC2), by producing several hydrolytic enzymes (amylase, pectinase, cellulase, protease, and chitinase) and by producing antifungal metabolites like siderophore and ammonia (Bencheikh et al, 2022). Based on all the aforementioned advantages of the antagonistic bacteria and their antifungal metabolites, they can be relied upon as ideal alternatives to chemical fungicides (Desmyttere et al, 2019;Ji et al, 2019).…”

Section: Table 3 Effect Of Tebuconazole and Bacillus Strains Againstmentioning

confidence: 99%

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Study of synergistic effect of combined application of tebuconazole with two biocontrol agents for management of Fusarium crown rot in durum wheat

et al. 2023

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The in vitro and growth chamber, tests were conducted in order to assess the effects of B. amyloliquefaciens B18 and B. subtilis S8 strains each alone and in combination with tebuconazole against F. culmorum (FC) isolate responsible of Fusarium crown rot (FCR) in durum wheat. The in vitro growth of B18 and S8 strains was unaffected by 30 µg.mL-1 tebuconazole. The Bacillus strains (at 106 CFU.mL-1) and tebuconazole, each alone, reduced the mycelial growth, this effect was significantly improved when they were combined (inhibition of more than 92 %). In growth chamber experiments, efficacy against FCR was significantly higher when integrating Bacillus strains and tebuconazole than by either alone; control efficacy of tebuconazole at 30 µg.mL-1 in combination with S8 and B18 strains reached 90.91 and 95.45 %, respectively. The obtained results indicated that combination of tebuconazole with the biocontrol agents B18 and S8 synergistically improved control efficiency of the fungicide against FCR of wheat.

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Unraveling the Seed Bio-priming Contours for Managing Plant Health

Kumar,

Arutselvan,

Greeshma

et al. 2024

J Plant Growth Regul

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Efficiency of durum wheat seeds biopriming by rhizobacteria in the biocontrol of Fusarium culmorum and Fusarium chlamydosporum infecting durum wheat in Algeria

Cited by 7 publications

References 42 publications

Pseudomonas plecoglossicida(NR_114226) as a novel biocontrol agent against Fusarium crown rot of wheat

Pseudomonas plecoglossicida(NR_114226) as a novel biocontrol agent against Fusarium crown rot of wheat

Study of synergistic effect of combined application of tebuconazole with two biocontrol agents for management of Fusarium crown rot in durum wheat

Unraveling the Seed Bio-priming Contours for Managing Plant Health

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