Efficiency of gold nanoparticles coated with the antimicrobial peptide indolicidin against biofilm formation and development of <em>Candida </em>spp. clinical isolates

Abstract

BackgroundThis article examines the use of a novel nano-system, gold nanoparticles coated with indolicidin (AuNPs–indolicidin), against pathogenic Candida albicans biofilms. Candida species cause frequent infections owing to their ability to form biofilms, primarily on implant devices.Materials and methodsWe used an integrated approach, evaluating the effect of AuNPs-indolicidin on prevention and eradication of Candida biofilms formed in multi-well polystyrene plates, with relative gene expression assays. Four biofilm-associated genes (FG1, HWP1, ALS1 and ALS3, and CDR1 and CDR2) involved in efflux pump were analyzed using reverse transcription polymerase chain reaction.ResultsTreatment with the nano-complex significantly inhibits the capacity of C. albicans to form biofilms and impairs preformed mature biofilms. Treatment with AuNPs–indolicidin results in an increase in the kinetics of Rhodamine 6G efflux and a reduction in the expression of biofilm-related genes.ConclusionThese data provide a chance to develop novel therapies against nosocomially acquired refractory C. albicans biofilms.

Eradication of Candida albicans persister cell biofilm by the membranotropic peptide gH625

“…obtained from samples collected during a previous surveillance study performed by our group 39 , were routinely maintained in Sabouraud dextrose agar (1% yeast extract, 1% peptone, 4% glucose, 1% agar) at 4 °C. To prepare a standard cell suspension, a single colony was inoculated into Tryptone Soya Broth (TSB) (Oxoid) medium with 1% of glucose and incubated overnight at 37 °C at 200 rpm.…”

Silver/silver chloride (Ag/AgCl) nanoparticles synthesized from Azadirachta indica lalex and its antibiofilm activity against fluconazole resistant Candida tropicalis

“…The fungal organisms in the biofilms are highly resistant toantifungal drugs [27]. Recently, many reports have been published on the nanoparticles are effective anti-biofilm agents against for both bacteria and fungi [28][29][30].…”

An Update on Antimicrobial Peptides (AMPs) and Their Delivery Strategies for Wound Infections

“…In another study, AuNPs functionalized with indolicidin were obtained through thiol chemistry by covalently attaching AMP and to AuNPs. The Au/indolicidin nanosystem presented higher efficacy in preventing C. albicans adhesion and eradicated biofilm formation in vitro, as compared to indolicidin alone [171]. The enhanced activity of indolicidin was attributed to the protection from protease degradation conferred by the incorporation into the NPs.…”