Efficiency of hyaluronic acid (HA) sperm selection

Parmegiani, Lodovico; Cognigni, Graçiela Estela; Ciampaglia, Walter; Pocognoli, Patrizia; Marchi, F.; Filicori, Marco

doi:10.1007/s10815-009-9380-0

Cited by 117 publications

(98 citation statements)

References 24 publications

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“…While studies have found HA bound sperm to be associated with low DNA fragmentation [7,8] there have been conflicting reports with regards to clinical outcome. While Nasr-Eshafani et al [8] reported an increase in fertilization rate and Parmegiani et al [9] showed an improvement in embryo quality and implantation rates after injection of HA bound sperm, Menezo et al [12] found no difference in embryo quality or clinical pregnancy rates in HA-ICSI as compared to PVP-ICSI.…”

Section: Discussionmentioning

confidence: 99%

“…It has also been reported that sperm that can bind to HA have a reduced risk of being anueploid [6] or having fragmented DNA [7,8]. Initially, a number of studies have suggested a beneficial effect of HA sperm selection on assisted reproductive technology (ART) outcomes [7][8][9][10][11]. However, recently a large study comparing ICSI using HA and conventional ICSI showed no statistical difference in embryo quality and pregnancy rates [12].…”

Section: Introductionmentioning

confidence: 99%

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A prospective randomized study to evaluate the effect of hyaluronic acid sperm selection on the intracytoplasmic sperm injection outcome of patients with unexplained infertility having normal semen parameters

Majumdar

2013

J Assist Reprod Genet

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Purpose Sperm quality plays an important role in determining embryo development and intracytoplasmic sperm injection (ICSI) outcome. Selection of competent sperm based on its ability to bind to hyaluronic acid (HA) has been suggested as one of the methods to assess sperm quality. The aim of the present study was to examine whether injection of HA bound sperm helps in improving outcome in patients undergoing ICSI with unexplained infertility having normal semen parameters. Methods Patients with unexplained infertility having normal semen parameters in accordance with WHO 2010 criterion, undergoing their first IVF-ICSI cycle were enrolled during the course of the study.156 patients were prospectively randomized after oocyte retrieval and were assigned to either the ICSI group, where sperm selection for injection was based on visual assessment, or the PICSI group, where sperm were selected based on their ability to bind to HA. Only fresh embryo transfers were included in the analysis. Results There was no difference in the fertilization rates, number of top quality embryos and clinical pregnancy rates between the ICSI and PICSI groups (65.7 % vs 64.7 %; 45.8 % vs 43.6 % and 35 % vs 35.2 % respectively). However, a higher pregnancy loss rate was observed in the ICSI group (25 % vs 12 %; P =0.227) as compared to the PICSI group, but the difference was not statistically significant. Implantation rates were 22.03 % and 18.84 % in the PICSI and ICSI groups respectively. There were 22 (31 %) live births in the PICSI group and 21(26.3 %) live births in the ICSI group. Conclusions Patients with unexplained infertility having normal semen parameters may constitute a patient group which does not benefit from this sperm selection method. A larger study may be necessary to establish a relationship between PICSI and pregnancy loss rate in patients undergoing IVF with unexplained infertility.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A prospective randomized study to evaluate the effect of hyaluronic acid sperm selection on the intracytoplasmic sperm injection outcome of patients with unexplained infertility having normal semen parameters

Majumdar

2013

J Assist Reprod Genet

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“…Studies documenting the use of sperm selected by HA method in the clinical ART setting are still scarce and somehow contradictory. While one study reported significantly increased fertilization rate of oocytes injected with HA-selected sperm and only a marginal effect on pregnancy rate (Nasr-Esfahani et al, 2008), in other studies by Permegiani et al (2010a;2010b) oocytes injected with sperm selected by the binding method originated better quality embryos but no effect was detected on fertilization and pregnancy rates.…”

Section: Hyaluronic Acid Sperm Bindingmentioning

confidence: 90%

Methods for Sperm Selection for In Vitro Fertilization

Ortega¹,

Bosch²

2012

In Vitro Fertilization - Innovative Clinical and Laboratory Aspects

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“…This, and related work, suggest that spermatozoa capable of binding to hyaluronic acid have completed their maturation processes, most specifically plasma membrane remodelling and full chromatin condensation, and subsequently have an increased fertilizing capacity (Cayli et al, 2003;Huszar et al, 2003;Mokanszki et al, 2014). Different studies have suggested that hyaluronic-acid-binding improves embryo quality and development after ICSI, although all these studies were relatively small (Nasr-Esfahani et al, 2008;Parmegiani et al, 2010a;Parmegiani et al 2010b;Mokanszki et al, 2014;Beck-Fruchter et al, 2015).…”

Section: Changes In Sperm Hyaluronic-acid-binding and Hyperactive Motmentioning

confidence: 97%

Sedimentation properties in density gradients correspond with levels of sperm DNA fragmentation, chromatin compaction and binding affinity to hyaluronic acid

Torabi

Binduraihem

Miller

2017

Reproductive BioMedicine Online

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ReuseUnless indicated otherwise, fulltext items are protected by copyright with all rights reserved. The copyright exception in section 29 of the Copyright, Designs and Patents Act 1988 allows the making of a single copy solely for the purpose of non-commercial research or private study within the limits of fair dealing. The publisher or other rights-holder may allow further reproduction and re-use of this version -refer to the White Rose Research Online record for this item. Where records identify the publisher as the copyright holder, users can verify any specific terms of use on the publisher's website. TakedownIf you consider content in White Rose Research Online to be in breach of UK law, please notify us by emailing eprints@whiterose.ac.uk including the URL of the record and the reason for the withdrawal request. Accepted ManuscriptTitle: Sedimentation properties in density gradients correspond with levels of sperm DNA fragmentation, chromatin compaction and binding affinity to hyaluronic acid 22-11-2016 Please cite this article as: Forough Torabi, Adel Binduraihem, David Miller, Sedimentation properties in density gradients correspond with levels of sperm DNA fragmentation, chromatin compaction and binding affinity to hyaluronic acid, Reproductive BioMedicine Online (2016), http://dx.doi.org/doi: 10.1016/j.rbmo.2016.11.011. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractMature spermatozoa bind hyaluronic acid in the extracellular matrix via hyaladherins.Immature spermatozoa may be unable to interact because they do not express the appropriate hyaladherins on their surface. Fresh human semen samples were fractionated using differential density gradient centrifugation (DDGC) and the ability of these fractions to bind hyaluronic acid was evaluated. The presence of sperm hyaladherins was also assessed. CD44 was located mainly on the acrosome and equatorial segment and became more restricted to the equatorial segment in capacitated spermatozoa. Hyaluronic acid-TRITC (hyaluronan acid conjugated with tetramethylrhodamine isothiocyanante), a generic hyaluronic-acid-binding reagent, labelled the membrane and the neck region, particularly after capacitation. Sperm populations obtained after DDGC or after interaction with hyaluronic acid were assessed for DNA fragmentation and chromatin maturity. Strong relationships between both measures and sperm sedimentation and hyaluronic-acid-binding profiles were revealed. Capacitation Overall success rates for assisted conception procedures, however (including ICSI), have remained relatively static, with about 24% of cycles for all age groups deliverin...

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Efficiency of hyaluronic acid (HA) sperm selection

Cited by 117 publications

References 24 publications

A prospective randomized study to evaluate the effect of hyaluronic acid sperm selection on the intracytoplasmic sperm injection outcome of patients with unexplained infertility having normal semen parameters

A prospective randomized study to evaluate the effect of hyaluronic acid sperm selection on the intracytoplasmic sperm injection outcome of patients with unexplained infertility having normal semen parameters

Methods for Sperm Selection for In Vitro Fertilization

Sedimentation properties in density gradients correspond with levels of sperm DNA fragmentation, chromatin compaction and binding affinity to hyaluronic acid

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