Efficiency of in vitro embryo production of yak (<i>Bos grunniens</i>) cultured in different maturation and culture conditions

Guo, Xian; Ding, Xuezhi; Pei, Jie; Bao, Pengjia; Liang, Chunnian; Chen, Min; Yan, Ping

doi:10.1080/09712119.2012.692327

Cited by 4 publications

(5 citation statements)

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“…IVP has been made great progress in cattle which is becoming one of the most exciting and progressive procedures available for today’s producers but the efficiency of yak IVP is still low, i.e. less than 10% of cleaved embryos (i.e., that were ≥2 cells) becoming blastocysts [ 13 , 14 ]. Therefore, this study was performed to investigate the effect of FSH, LH and MG132 during oocytes maturation on competence for development after fertilization in the yak.…”

Section: Introductionmentioning

confidence: 99%

Effect of addition of FSH, LH and proteasome inhibitor MG132 to in vitro maturation medium on the developmental competence of yak (Bos grunniens) oocytes

Xiao

Niu

et al. 2014

Reprod Biol Endocrinol

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BackgroundThe competence for embryonic development after IVF is low in the yak, therefore, we investigated the effects of supplementation of FSH, LH and the proteasome inhibitor MG132 in IVM media on yak oocyte competence for development after IVF.MethodsIn Experiment 1, yak cumulus-oocyte complexes (COCs) were in vitro matured (IVM) in TCM-199 with 20% fetal calf serum (FCS), 1 microg/mL estradiol-17beta, and different combinations of LH (50 or 100 IU/mL) and FSH (0, 1, 5, 10 microg/mL) at 38.6 degrees C, 5% CO2 in air for 24 h. Matured oocytes were exposed to frozen–thawed, heparin-capacitated yak sperm. Presumptive zygotes were cultured in SOF medium containing 6 mg/ml BSA, 0.5 mg/mL myoinositol, 3% (v/v) essential amino acids, 1% nonessential amino acids and 100 μg/mL L-glutamine (48 h, 38.5 degrees C, 5% CO2, 5% O2, and 90% N2). In Experiment 2, cumulus cells were collected at the end of IVM to determine FSHR and LHR mRNA expression by real-time PCR. In Experiment 3 and 4, COCs were cultured in the presence or absence of the proteasomal inhibitor MG132 from either 0–6 h or 18–24 h after initiation of maturation.ResultsThe optimum concentration of FSH and LH in IVM media was 5 microg/mL FSH and 50 IU/mL LH which resulted in the greatest cleavage (79.1%) and blastocyst rates (16.1%). Both FSHR and LHR mRNA were detected in yak cumulus cells after IVM. Treatment with MG132 early in maturation reduced (P < 0.05) cleavage and blastocyst rates. Conversely, treatment with MG132 late in maturation improved (P < 0.05) blastocyst rate. Optimal results with MG132 were achieved at a concentration of 10 microM.ConclusionsAn optimum concentration of FSH and LH in IVM medium, and treatment with MG132 late in maturation can improve yak oocytes competence for development after IVF.

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Section: Introductionmentioning

confidence: 99%

Effect of addition of FSH, LH and proteasome inhibitor MG132 to in vitro maturation medium on the developmental competence of yak (Bos grunniens) oocytes

Xiao

Niu

et al. 2014

Reprod Biol Endocrinol

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“…The reason behind the loss of the established yak pregnancies remains unclear. Possible explanations for the failure of pregnancies are the poor developmental competence of frozen–thawed yak embryos, utero‐placental compatibility, low temperature, hypoxia, scarce vegetation, high altitude and other harsh natural conditions (Guo et al, ). The successful cryopreservation of bovine embryos could facilitate the widespread use and increase the potential benefits of embryo transfer Campos‐Chillon et al, ; Pugh, Tervit, & Niemann, ).…”

Section: Discussionmentioning

confidence: 99%

“…The direct transfer of the embryos involves warming and dilution (Inaba et al, 2011) or additional steps to transfer the embryo from the cryodevice to the straw (Vieira, Forell, Feltrin, & Rodrigues, 2007). (Guo et al, 2012). The successful cryopreservation of bovine embryos could facilitate the widespread use and increase the potential benefits of embryo transfer Campos-Chillon et al, 2006;Pugh, Tervit, & Niemann, 2000).…”

Section: Discussionmentioning

confidence: 99%

“…In this region and its surrounding mountainous areas, the yak is a unique and valuable domestic animal. The yak is characterized by its adaptability to low temperature, hypoxia, sparse vegetation, high altitude, and other harsh environmental conditions, which seriously affect its reproductive efficiency (Guo et al, ,). The reproductive characteristics of yaks may also be influenced by human factors, including human‐invaded habitat, irreversible decline and destruction of the ecological environment, and human‐organized hunting (Ma, ; Yao et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Cryosurvival and pregnancy rates: One‐step protocol for freezing–thawing Shangri‐la Yak (Bos grunniens) Embryos

Jia

Yang

Zhang

et al. 2018

Reprod Domestic Animals

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The yak is one of the most important and economically useful animals for highlanders. The decline in the yak population requires effective measures for the conservation and multiplication of elite germplasm. A standardized protocol will simplify the freezing and warming of yak embryos in straw and facilitate embryo transfer. In this work, we investigated a one-step protocol that uses a stable basal medium, which comprised a warming medium (1.08 M sucrose) and a freezing medium (EFS40). We also assessed the effects of the new transfer method on embryo survival. A total of 145 yak frozen embryos were thawed in a standard medium system. The one-step protocol led to a high recovery percentage (84.93) of yak embryos that survived vitrification and warming. The in vitro survival rates of these embryos significantly different from those of embryos frozen-thawed via the conventional method. The 95 embryos frozen-thawed via our one-step protocol were then implanted in selected recipients. Thirty-six singleton pregnancies were established. In conclusion, the proposed one-step method is a simple, safe, and standardized freezing-thawing protocol that ensures embryo survival and quality under field conditions. This study establishes new possibilities for the widespread use of embryo transfer in yaks.

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“…Reproduction in yaks is low as a result of seasonal breeding, delayed puberty, and lower estrus frequency. Therefore, numerous recent studies have focused on reproduction and physiology in yaks, as well as assisted reproductive techniques ( Sarkar et al, 2008 ; Guo et al, 2012 ). Follicular development is a very complicated process; it plays a role in oocyte maturation and the ovulation mechanism during yak breeding season.…”

Section: Discussionmentioning

confidence: 99%

Comparative Proteomic Analysis of Yak Follicular Fluid during Estrus

Guo

Pei

Ding

et al. 2015

Asian Australas. J. Anim. Sci

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The breeding of yaks is highly seasonal, there are many crucial proteins involved in the reproduction control program, especially in follicular development. In order to isolate differential proteins between mature and immature follicular fluid (FF) of yak, the FF from yak follicles with different sizes were sampled respectively, and two-dimensional gel electrophoresis (2-DE) of the proteins was carried out. After silver staining, the Image Master 2D platinum software was used for protein analysis and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) was performed for differential protein identification. The expression level of transferrin and enolase superfamily member 1 (ENOSF1) was determined by Western blotting for verification analysis. The results showed that 2-DE obtained an electrophoresis map of proteins from mature and immature yak FF with high resolution and repeatability. A comparison of protein profiles identified 12 differently expressed proteins, out of which 10 of them were upregulated while 2 were downregulated. Western blotting showed that the expression of transferrin and ENOSF1 was enhanced with follicular development. Both the obtained protein profiles and the differently expressed proteins identified in this study provided experimental data related to follicular development during yak breeding seasons. This study also laid the foundation for understanding the microenvironment during oocyte development.

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Efficiency of in vitro embryo production of yak (Bos grunniens) cultured in different maturation and culture conditions

Abstract: View related articles Citing articles: 3 View citing articles Efficiency of in vitro embryo production of yak (Bos grunniens) cultured in different maturation and culture conditions

Cited by 4 publications

References 21 publications

Effect of addition of FSH, LH and proteasome inhibitor MG132 to in vitro maturation medium on the developmental competence of yak (Bos grunniens) oocytes

Effect of addition of FSH, LH and proteasome inhibitor MG132 to in vitro maturation medium on the developmental competence of yak (Bos grunniens) oocytes

Cryosurvival and pregnancy rates: One‐step protocol for freezing–thawing Shangri‐la Yak (Bos grunniens) Embryos

Comparative Proteomic Analysis of Yak Follicular Fluid during Estrus

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