Efficiency of Lecanicillium lecanii to control the tick Rhipicephalus microplus

Angelo, Isabele da Costa; Fernandes, Éverton K. K.; Bahiense, Thiago Campanharo; Perinotto, Wendell Marcelo de Souza; Moraes, Ana Paula Rodrigues; Terra, A. L. M.; Bittencourt, Vânia Rita Elias Pinheiro

doi:10.1016/j.vetpar.2010.04.038

Cited by 38 publications

(23 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Mineral and vegetable oils are used in formulations as adjuvants to protect conidia and to maximise fungal performance . Extensive research showed that oil formulations are more efficacious than aqueous formulations or wettable powders under various temperature and moisture conditions . In our experiment, compared with wettable powders, oil‐based formulations of I. fumosorosea were more effective against P. xylostella at the same conidial concentrations.…”

Section: Discussionmentioning

confidence: 54%

Evaluation of alternativePlutella xylostellacontrol by twoIsaria fumosoroseaconidial formulations - oil-based formulation and wettable powder - combined withBacillus thuringiensis

Nian

Lü

et al. 2015

Pest. Manag. Sci.

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 54%

Evaluation of alternativePlutella xylostellacontrol by twoIsaria fumosoroseaconidial formulations - oil-based formulation and wettable powder - combined withBacillus thuringiensis

Nian

Lü

et al. 2015

Pest. Manag. Sci.

View full text Add to dashboard Cite

show abstract

“…Differential susceptibility among different developmental stages in ticks has already been reported by many workers (Kaaya et al 1996;Samish et al 2001;Angelo et al 2010). Conidia of M. anisopliae formulated in emulsifiable leaf extract of C. aurea did not result in any synergism effect against tick as reported in the case of B. bassiana and neem against Spodoptera litura Fabricius (Mohan et al 2007).…”

Section: Discussionmentioning

confidence: 56%

Compatibility between Calpurnia aurea leaf extract, attraction aggregation, and attachment pheromone and entomopathogenic fungus Metarhizium anisopliae on viability, growth, and virulence of the pathogen

et al. 2011

View full text Add to dashboard Cite

Metarhizium anisopliae sensu stricto (ss) (Metsch.) Sorok. isolate ICIPE 07 is being developed as biopesticide for the control of ticks. In addition, leaf extracts of Calpurnia aurea Benth, and the attraction aggregation and attachment pheromone (AAAP) are being used as ticks' attractant. The three agents are being considered for use in combination in an autodissemination approach, whereby ticks that are attracted to semiochemicals are infected with the inoculum. Experiments were therefore conducted to evaluate in vitro the compatibility between C. aurea, AAAP, and the M. anisopliae on vegetative growth, conidial production, and spore viability. Calpurnia aurea leaf extract was compatible with the fungus at all the concentrations tested, whereas AAAP inhibited all the fungal growth parameters. The virulence of M. anisopliae formulated in emulsifiable extracts of C. aurea was also tested against different developmental stages of Rhipicephalus appendiculatus in laboratory bioassays. No significant differences in virulence were observed between M. anisopliae applied alone and M. anisopliae formulated in different concentrations of C. aurea leaf extracts. These results suggest that C. aurea leaf extracts is compatible with M. anisopliae and could be mixed together for "spot-spray" treatments as lowcost and environmental-friendly technology to control ticks in grazing field, while AAAP should be used separately.

show abstract

“…Most studies on microbial control of ticks available report the performance of fungal formulations in bioassays held in optimal conditions of temperature and RH (Kaaya and Hassan, 2000;Maranga et al, 2005;Lopes et al, 2007;Fernandes and Bittencourt, 2008;Angelo et al, 2010;Camargo et al, 2012). The current study, however, reports the performance against the tick R. sanguineus of M. anisopliae formulated in oil or water, and exposed or not exposed to heat stress.…”

Section: Discussionmentioning

confidence: 80%

“…Likewise, formulating conidia of M. anisopliae, B. bassiana or Lecanicillium lecanii (Angelo et al, 2010) in mineral oil increased the efficacy of these fungi in controlling various life stages of R. microplus. Oil-based formulation ingredients can be toxic to arthropods, such as the mortality of R. microplus engorged females treated with coconut oil (Polar et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, oil extends the viability of conidia held at ambient conditions or under refrigeration (Prior et al, 1988). The positive effects of formulating entomopathogenic fungi in oil have been reported as, (a) increased fungus-induced mortality against cocoa weevil (Prior et al, 1988), triatomine bugs (Luz and Batagin, 2005), grasshoppers (Lomer et al, 2001) and whiteflies (Malsam et al, 2002); (b) increased ovicidal activity against Aedes aegypti mosquito (Albernaz et al, 2009) and Rhipicephalus annulatus tick (Samish et al, 2014), and (c) increased virulence against different life stages of the tick R. microplus (Angelo et al, 2010;Camargo et al, 2012).…”

Section: Rhipicephalus Microplusmentioning

confidence: 99%

See 1 more Smart Citation

Effect of heat stress and oil formulation on conidial germination of Metarhizium anisopliae s.s. on tick cuticle and artificial medium

Barreto

Luz

Mascarin

et al. 2016

Journal of Invertebrate Pathology

Self Cite

View full text Add to dashboard Cite

The effect of heat stress (45°C) versus non-heat stress (27°C) on germination of Metarhizium anisopliae sensu stricto (s.s.) isolate IP 119 was examined with conidia formulated (suspended) in pure mineral oil or in water (Tween 80, 0.01%), and then applied onto the cuticle of Rhipicephalus sanguineus sensu lato (s.l.) engorged females or onto culture medium (PDAY). In addition, bioassays were performed to investigate the effect of conidia heated while formulated in oil, then applied to blood-engorged adult R. sanguineus females. Conidia suspended in water then exposed to 45°C, in comparison to conidia formulated in mineral oil and exposed to the same temperature, germinated less and more slowly when incubated on either PDAY medium or tick cuticle. Also, conidial germination on tick cuticle was delayed in comparison to germination on artificial culture medium; for example, germination was 13% on tick cuticle 72h after inoculation, in contrast to 61.5% on PDAY medium. Unheated (27°C) conidia suspended in either water or oil and applied to tick cuticle developed appressoria 36h after treatment; whereas only heat-stressed conidia formulated in oil developed appressoria on tick cuticle. In comparison to conidia heated in mineral oil, there was a strong negative effect of heat on germination of conidia heated in water before being applied to arthropod cuticle. Nevertheless, bioassays [based primarily on egg production (quantity) and egg hatchability] exhibited high percentages of tick control regardless of the type of conidial suspension; i.e., water- or oil-formulated conidia, and whether or not conidia were previously exposed to heat. In comparison to aqueous conidial preparations, however, conidia formulated in oil reduced egg hatchability irrespective of heat or no-heat exposure. In conclusion, mineral-oil formulation protected conidia against heat-induced delay of both germination and appressorium production when applied to the cuticle of R. sanguineus.

show abstract

Efficiency of Lecanicillium lecanii to control the tick Rhipicephalus microplus

Cited by 38 publications

References 20 publications

Evaluation of alternativePlutella xylostellacontrol by twoIsaria fumosoroseaconidial formulations - oil-based formulation and wettable powder - combined withBacillus thuringiensis

Evaluation of alternativePlutella xylostellacontrol by twoIsaria fumosoroseaconidial formulations - oil-based formulation and wettable powder - combined withBacillus thuringiensis

Compatibility between Calpurnia aurea leaf extract, attraction aggregation, and attachment pheromone and entomopathogenic fungus Metarhizium anisopliae on viability, growth, and virulence of the pathogen

Effect of heat stress and oil formulation on conidial germination of Metarhizium anisopliae s.s. on tick cuticle and artificial medium

Contact Info

Product

Resources

About