Efficiency of magnetic immobilization for recombinant <i>Pichia pastoris</i> cells harvesting over consecutive production cycles

Taghizadeh, Seyedeh-Masoumeh; Ghoshoon, Mohammad Bagher; Ghasemi, Younes; Dehshahri, Ali; Berenjian, Aydin; Ebrahiminezhad, Alireza

doi:10.1080/01496395.2022.2121725

Cited by 5 publications

(1 citation statement)

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“…They demonstrated that the nanoparticles adhered to the cell surface without adversely affecting the growth and viability of R. toruloides. Taghizadeh et al [91] immobilized recombinant Pichia pastoris cells on magnetic magnetite nanoparticles coated with aminopropyltriethoxy silane, and also found that magnetic immobilization did not affect cell growth or viability during three cycles of reuse of the immobilized cells. A study was carried out in a magnetically assisted bioreactor, with the addition of Fe 3 O 4 nanoparticles, to produce ethanol using the yeast Saccharomyces cerevisiae, demonstrating that the maximum specific growth rate showed a positive effect in this magnetically assisted bioprocess [92].…”

Section: Fermentation By Adhesion To Magnetic Nanoparticlesmentioning

confidence: 99%

Current Advances in Carotenoid Production by Rhodotorula sp.

Ochoa-Viñals,

Alonso-Estrada,

Pacios-Michelena

et al. 2024

Fermentation

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Microbial carotenoids are pigments of lipophilic nature; they are considered promising substitutes for chemically synthesized carotenoids in the food industry. Their benefits for human health have been demonstrated due to their antioxidant capacity. Yeasts of the genus Rhodotorula have genotypic characteristics that allow them to accumulate high concentrations of carotenes under certain stress conditions. The present review includes recent information covering different aspects of carotenoid production in Rhodotorula sp. fermentation. This review focuses on fermentation carotenoid production strategies, describing various economic raw materials as sources of carbon and nitrogen, the capacity for tolerance to heavy metals, and the effect of light, pH, and salts on the accumulation of carotenoids. Genetic modification strategies used to obtain strains with increased carotenoid production are described. Furthermore, using magnetic nanoparticles in the fermentation system, which could be a stress factor that increases pigment production, is considered for the first time. Rhodotorula is a potential source of high-value carotenoids with applications in the cosmetics, pharmaceutical, and food industries.

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Section: Fermentation By Adhesion To Magnetic Nanoparticlesmentioning

confidence: 99%