Efficiency of MicroSort flow cytometry for producing sperm populations enriched In X- or Y-chromosome haplotypes: a blind trial assessed by double and triple colour fluorescent in-situ hybridization

Vidal, Francesca; Fugger, Edward F.; Blanco, Joan; Keyvanfar, Keyvan; Català, Vicenç; Norton, Mary E.; Hazelrigg, W. Brent; Black, Susan H.; Levinson, Gene; Egozcue, J.; Schulman, Joseph D.

doi:10.1093/humrep/13.2.308

Cited by 35 publications

(21 citation statements)

References 36 publications

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“…The use of DNA probes in conjunction with fluorescent in situ hybridization (FISH) has shown that flow cytometric sorting is 82% accurate for sorting the X-sperm and 75% accurate for the Y-sperm [19]. More recently, however, the flow cytometric sorting method was reported to have 87% and 67% accuracy rates in sorting for X-and Y-sperm, respectively [20].…”

Section: Discussionmentioning

confidence: 99%

Sperm Velocity in Seminal Plasma and the Association With Gender of Offspring

Balli

Patton

Jacobson

et al. 2004

Archives of Andrology

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The gender of the offspring is determined by the fertilizing sperm. Previous gender studies were based on washed sperm, but not on sperm in seminal plasma. The objective was to correlate motility parameters assessed during semen analyses with the offspring gender. For comparison, fixed sperm head DNA quantitated by Hoechst 33342 fluorescence microscopy was also analyzed. Forty-six patients undergoing assisted reproduction procedures resulted in livebirth deliveries with either male or female-predominant offsprings. Sperm head fluorescence was weakly correlated to the gender in 61% of the cases. Sperm of patients with male offsprings had slower curvilinear (44.2 AE 1.8 mean AE SEM, versus, 49.9 AE 2.7 m=sec) and slower average path velocities (32.4 AE 1.2 versus 36.3 AE 1.7 m=sec). Using cut-off values for the curvilinear ( < 49 m=sec) and average path ( < 36 m=sec) velocities of sperm swimming in seminal plasma, the two parameters predicted 75 and 68% of the male offspring births, respectively. The data suggest that sperm movement in seminal plasma is a marker for factors that skew the ratio of the X-to Y-sperm populations.

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Section: Discussionmentioning

confidence: 99%

Sperm Velocity in Seminal Plasma and the Association With Gender of Offspring

Balli

Patton

Jacobson

et al. 2004

Archives of Andrology

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“…The two methods currently commercially available are sperm separation and preimplantation genetic diagnosis (PGD) [1][2][3][4][5][6]. Even though the intended use of such technologies was to prevent the birth of children with sexlinked genetic disorders, these technologies are being increasingly used for preimplantation sex selection for nonmedical reasons (http://www.microsort.net/index.php, accessed March 1, 2007) [7,8].…”

Section: Introductionmentioning

confidence: 99%

Preimplantation sex selection demand and preferences among infertility patients in Midwestern United States

Missmer

Jain

2007

J Assist Reprod Genet

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Purpose To determine the demand and preferences of infertility patients for sex selection for nonmedical reasons, and to investigate the relation between these choices and their demographic and socioeconomic characteristics. Methods A cross-sectional, self-administered survey by mail was conducted at a University hospital-based fertility center of 1,350 consecutive women who presented for infertility care, to assess patient demand and preferences for sex selection. Results Of respondents, 49% wanted to select the sex of their next child for no added cost. Of these patients, 56% had no living children and 37% had children all of one sex. After adjustment for observed predictors of gender preference, we found a significant preference for a female child among women who had only sons, had more living children, or were single. Nulliparous women did not significantly prefer one sex over the other. Among parous women, those with only daughters significantly desired to select a male child, whereas those with sons significantly desired to select a female child. Conclusion There is significant demand among infertility patients for preimplantation sex selection, with a significant portion of this demand coming from patients who do not have any children or have children all of one sex.

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“…The technology employs a flow cytometer that separates the 2.8% heavier X-from Y-bearing sperm to produce an X-or Y-enriched sperm sample for artificial insemination or in vitro fertilization (2)(3)(4). Upon successful completion of the clinical trial (which began in 1995), the potential exists for widespread dissemination and marketing of this technology throughout the United States (5).…”

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confidence: 99%

Preconception sex selection demand and preferences in the United States

Dahl

Gupta

Beutel

et al. 2006

Fertility and Sterility

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Objective: Preconception sex selection for nonmedical reasons raises important moral, legal, and social issues. The main concern is based upon the assumption that a widely available service for sex selection will lead to a socially disruptive imbalance of the sexes. For a severe sex ratio distortion to occur, however, at least two conditions have to be met. First, there must be a significant preference for children of a particular sex, and second, there must be a considerable interest in employing sex selection technology. Our objective was to ascertain such demand and preferences among the United States general population. Design: Cross-sectional web-based survey. Setting: United States general population. Patient(s):One thousand one hundred ninety-seven men and women aged 18 to 45 years. Intervention(s): None. Main Outcome Measure(s): Web-based questionnaire assessing preferences for sex of children and demand for preconception sex selection for nonmedical reasons. Result(s):Eight percent of respondents would use preconception sex selection technology, 74% were opposed, and 18% were undecided. If the sex selection process was simplified to taking a pill, 18% would be willing to use such a medication, 59% were opposed, and 22% were undecided. In terms of gender choices, 39% of respondents would like their first child to be a son, 19% would like their first child to be a daughter, and 42% had no preference. Overall, 50% wished to have a family with an equal number of boys and girls, 7% with more boys than girls, 6% with more girls than boys, 5% with only boys, 4% with only girls, and 27% had no preference. Conclusion(s): Preconception sex selection technology via sperm separation is unlikely to be used by the majority of the United States population and is unlikely to have a significant impact on the natural sex ratio. (Fertil Steril 2006;85:468 -73.

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Efficiency of MicroSort flow cytometry for producing sperm populations enriched In X- or Y-chromosome haplotypes: a blind trial assessed by double and triple colour fluorescent in-situ hybridization

Cited by 35 publications

References 36 publications

Sperm Velocity in Seminal Plasma and the Association With Gender of Offspring

Sperm Velocity in Seminal Plasma and the Association With Gender of Offspring

Preimplantation sex selection demand and preferences among infertility patients in Midwestern United States

Preconception sex selection demand and preferences in the United States

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