In South Africa, lack of access to clean and safe water is one of the main causes of poverty because it makes agriculture, livestock raising cattle, forestry, fisheries, hydropower, and other innovative activities unstainable. This requires sustainable solution to address the present water crisis via the coagulation pretreatment process. Herein, using natural coagulants comes in handy to replace chemical coagulants, due to their potential of nontoxicity, biodegradability, readily available, and eco-friendly. Therefore, this study investigated the preparation and characterization of natural coagulants using bio-waste materials. This included banana peel (BP), eggshells (ES), and seashells (SS) and their calcinated counterparts. Brunauer-Emmett-Teller (BET), Fourier Transform Infrared (FTIR), and energy dispersive X-ray (EDX) were used to investigate the properties of the natural coagulants. The calcined coagulants are suited for water treatment and based on characterization, calcined banana peels were superior to the others. The optimum conditions for the calcination temperature of BP were 400℃ for a retention time of 2 hours and a temperature of 800℃ at a retention time of 3 hours for SS and ES. The findings demonstrate that the calcined banana peel had a larger surface area and pore size of 4.3889 m 2 /g and 3.167 Å, respectively. Additionally, it had a potassium content of 34.1 wt.%, and the FTIR results suggest that the functional group OH may have been present. These factors make this bio-coagulant superior to other bio-coagulants, and hence, it would be beneficial for wastewater treatment.