Efficiency of pretreatments for optimal enzymatic saccharification of soybean fiber

Karki, Bishnu; Maurer, Devin L.; Jung, Stéphanie

doi:10.1016/j.biortech.2011.03.014

Cited by 39 publications

(17 citation statements)

References 29 publications

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“…The hydrolytic (enzymatic) pretreatment is meant to increase the susceptibility of the substrate to biological degradation, increasing the rate of the methane fermentation process and the efficacy of the biogas obtained. To improve the biodegradability of the wastes, a variety of methods, such as chemical, physicochemical, biological, and combinations thereof, have been employed (Demirbas 2007;Bruni et al 2010;Karki et al 2011;Parawira 2012).…”

Section: Introductionmentioning

confidence: 99%

Characterization and Influence of a Multi-enzymatic Biopreparation for Biogas Yield Enhancement

Oszust¹,

Pawlik²,

Janusz³

et al. 2017

BioResources

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A multi-enzymatic biopreparation of Trichoderma atroviride G79/11 origin was characterized. The fungus showed relatively high cellulase production in a soybean flour-cellulose-lactose medium. Subsequently, based on its post-culture liquid, the biopreparation of the enzyme mixture was developed and characterized. The liquid form of the enzyme mixture reached 22 U cm -3 of cellulolytic activity and its lyophilisate exhibited 1.09 U cm -3 at pH 5.1 and 50 °C. The enzyme mixture was characterized by the following activities: xylanase, β-glucosidase, carboxymethyl cellulase, polygalactouronase, pectinesterase, amylase, lactase, and protease. A method for an efficient conditioning process of organic waste (fruit processing waste, dairy sewage sludge, corn silage, and grain broth) for biogas yield enhancement using the enzyme mixture was proposed. The enzyme mixture increased the efficacy of biogas production by 30% when the lyophilizate (0.5 mg g -1 d.m.) was applied prior to fermentation. A method for conducting the enzymatic conditioning process of organic waste using the enzyme mixture as a pretreatment was proposed. This was part of the optimization of the methane fermentation process to increase the biogas yield. Consequently, after application of the biopreparation, the efficiency of anaerobic digestion of organic waste was improved.

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Section: Introductionmentioning

confidence: 99%

Characterization and Influence of a Multi-enzymatic Biopreparation for Biogas Yield Enhancement

Oszust¹,

Pawlik²,

Janusz³

et al. 2017

BioResources

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“…[33] The authors attributed this to the fact that the starting material had already a completely disrupted cellular structure of the extruded soybean flakes and that ultrasound did not contribute to further disintegration of the cellular matrix. For example, the chemical pretreatment (acid or basic) of soybean fiber showed significant lignin degradation, but ultrasonic pretreatment (20 kHz, maximum power output 2200 W) was inefficient.…”

Section: Sonochemical Pretreatmentmentioning

confidence: 99%

“…For example, the chemical pretreatment (acid or basic) of soybean fiber showed significant lignin degradation, but ultrasonic pretreatment (20 kHz, maximum power output 2200 W) was inefficient. [33] The authors attributed this to the fact that the starting material had already a completely disrupted cellular structure of the extruded soybean flakes and that ultrasound did not contribute to further disintegration of the cellular matrix. In this case, another explanation could be that the optimum sonochemical conditions had not been determined.…”

Section: Sonochemical Pretreatmentmentioning

confidence: 99%

Sonochemistry: What Potential for Conversion of Lignocellulosic Biomass into Platform Chemicals?

2014

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This Review focuses on the use of ultrasound to produce chemicals from lignocellulosic biomass. However, the question about the potential of sonochemistry for valorization/conversion of lignocellulosic biomass into added-value chemicals is rather conceptual. Until now, this technology has been mainly used for the production of low-value chemicals such as biodiesel or as simple method for pretreatment or extraction. According to preliminary studies reported in literature, access to added-value chemicals can be easily and sometimes solely obtained by the use of ultrasound. The design of sonochemical parameters offers many opportunities to develop new eco-friendly and efficient processes. The goal of this Review is to understand why the use of ultrasound is focused rather on pretreatment or extraction of lignocellulosic biomass rather than on the production of chemicals and to understand, through the reported examples, which directions need to be followed to favor strategies based on ultrasound-assisted production of chemicals from lignocellulosic biomass. We believe that ultrasound-assisted processes represent an innovative approach and will create a growing interest in academia but also in the industry in the near future. Based on the examples reported in the literature, we critically discuss how sonochemistry could offer new strategies and give rise to new results in lignocellulosic biomass valorization.

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“…Besides NaOH, other alkaline solution, NH4OH was tried in order to weaken the soybean fiber and thus enzymatic saccharification increased markedly (Karki et al, 2011). In this experiment, saccharification was more produced from the treatment with NH4OH than that with NaOH.…”

Section: Introductionmentioning

confidence: 99%

A New Method of Extracting Whole Cell Proteins from Soil Microorganisms Using Pre-treatment of Ammonium Hydroxide

Kang¹,

Kim²,

Roh³

et al. 2013

Journal of Applied Biological Chemistry

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Efficient extraction of total proteins from soil microorganisms is tedious because of small quantity. In this regard, an improved method for extraction of whole cell proteins is developed from soil microorganisms, Saccharomyces cerevisiae and Pichia pastoris. of which the cell wall are very strong. Pretreatment with NH4OH prior to the final extraction using NaOH/ SDS was tried under the basis that ammonium ion was possible to enhance the permeability and/or to weaken the yeast cell walls. The pre-treatment of yeast cells with NH4OH drastically enhanced the protein extraction when it was compared with control (without NH4OH pre-treatment). At the pre-treatment of 0.04 N NH4OH at pH 9.0, about 3 fold of proteins was obtained from p. pastoris. Ammonium hydroxide appears to penetrate into the yeast cell walls more readily at basic pH. The effect of NH4OH pretreatment was pH dependent. The methods developed in this experiment might be applicable for an effective extraction of yeast proteins for the purpose of biochemical studies, especially proteomic analysis.

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Efficiency of pretreatments for optimal enzymatic saccharification of soybean fiber

Cited by 39 publications

References 29 publications

Characterization and Influence of a Multi-enzymatic Biopreparation for Biogas Yield Enhancement

Characterization and Influence of a Multi-enzymatic Biopreparation for Biogas Yield Enhancement

Sonochemistry: What Potential for Conversion of Lignocellulosic Biomass into Platform Chemicals?

A New Method of Extracting Whole Cell Proteins from Soil Microorganisms Using Pre-treatment of Ammonium Hydroxide

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