Efficiency of RepeatedIn VivoOocyte and Embryo Recovery After rhFSH Treatment in Rabbits

Abstract

This study aims to assess the efficiency of in vivo oocyte and embryo recovery after a recombinant human FSH (rhFSH) treatment in rabbit does. Females were distributed in two experimental groups: donor does were treated with rhFSH (superovulation group) for 3 days prior to artificial insemination (embryo recovery) or ovulation induction (oocyte recovery) and does without treatment remained as the control group. Mature oocytes or embryos were collected with the laparoscopy technique 16 h after ovulation induction (oocytes) or 72 h after artificial insemination (embryos). Up to four recoveries were performed with each doe. Recovery efficiencies differed significantly between embryos (84%) and oocytes (58%). Yet, the recovery rates for the superovulation and control groups did not differ. The rhFSH group was associated with a significant increase (p < 0.05) in the number of oocytes and embryos recovered in comparison with the control group (10.2 +/- 1.0 and 14.3 +/- 1.2 vs 6.0 +/- 2.7 and 8.4 +/- 2.3 for oocytes and embryos, respectively). Results from this study indicate that repeated in vivo oocyte and embryo recovery from rhFSH superovulated does maximizes the number of oocytes or embryos collected from the same female.

Highly expressed recombinant human follicle-stimulating hormone from Chinese hamster ovary cells grown in serum-free medium and its effect on induction of folliculogenesis and ovulation

“…Although LH has been known as the primary hormone essential for ovulation and luteinization, studies using purified FSH preparations have suggested that FSH is capable of inducing ovulation in rats and hamsters (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13). Additionally, studies in hypophysectomized rats using recombinant human FSH (rhFSH) have confirmed that folliculogenesis and ovulation were induced by rhFSH alone (14,15).…”

Efecto De La Aplicación De Gonadotropinas Recombinantes Humanas Sobre La Producción Y Calidad De Ovocitos Y Embriones en Coneja

“…Given that it has been observed that FSH in absence of LH could induce superovulatory response in rabbit does both using purified porcine FSH or recombinant human FSH Cortell et al, 2008;Viudes de Castro et al, 2009), in the present study, the effect of these hormonal preparations on ovulation rate and oocyte quality was compared. Regarding to superovulatory response, both purified pFSH administered in five injections, twice a day (pFSH5), and rhFSH dissolved in PVP administered in three injections, once a day for three days (rhFSH3), resulted in a higher ovulation rate than control group.…”

“…In rabbits, superovulation protocols have been performed widely with eCG or FSH (Kauffmann et al, 1998;Cortell et al, 2008;Viudes de Castro et al, 2009;Viudes de Castro et al, 2010). Nevertheless, the recovery efficiency and oocyte quality of these superovulation treatments are highly variables.…”

“…However, in order to limit animal manipulation, superovulation treatments with FSH diluted in polyvinylpyrrolidone (PVP) solutions have been tested and have resulted in good superovulation response in distinct species, including rabbits Cortell et al, 2008;Viudes de Castro et al, 2009;Viudes de Castro et al, 2010).…”

“…In rabbits, the effect of LH on superovulation has been studied using both porcine purified FSH and human recombinant FSH (Cortell et al, 2008;Viudes de Castro et al, 2009;Viudes de Castro et al, 2010). In all cases, superovulatory response has been achieved; however, it would be very useful to determine how oocyte quality may be affected by these superovulation procedures.…”