Efficiency of selection for resistance to Fusarium wilt race 4 in cotton when conducted in the field versus greenhouse

Zhang, Jinfa; Elkins‐Arce, Heather D.; Wheeler, Terry A.; Dever, Jane K.; Whitelock, Derek P.; Wedegaertner, Tom; Hake, Kater; Bissonnette, Kaitlyn

doi:10.1007/s10681-022-03117-6

Cited by 5 publications

(2 citation statements)

References 43 publications

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“…Once adequate seeds from these germplasm lines are obtained, they will be further evaluated for FOV4 resistance under field (Fabens, Texas) conditions and made publicly available. Based on a set of eight cotton cultivars, we (Zhang et al 2022b, c, f) previously demonstrated that evaluation results for FOV4 resistance in the greenhouse were consistent with these under the field (Fabens, Texas) conditions, but with a lower experimental errors.…”

Section: Resultsmentioning

confidence: 58%

Targeted development of diagnostic SNP markers for resistance to Fusarium wilt race 4 in Upland cotton (Gossypium hirsutum)

Zhang

Wheeler²,

Dever³

et al. 2023

Preprint

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Fusarium wilt caused by the soil-borne fungus Fusarium oxysporum f. sp. vasinfectum (FOV) race 4 (FOV4) has become one of the most important emerging diseases in US cotton production. Numerous QTLs have been reported for resistance to FOV; however, no major FOV4-resistance QTL or gene has been identified and used in breeding Upland cotton (Gossypium hirsutum) for FOV4 resistance. In this study, a panel of 223 Chinese Upland cotton accessions was evaluated for FOV4 resistance based on seedling mortality rate (MR) and stem and root vascular discoloration (SVD and RVD). SNP markers were developed based on targeted genome sequencing using AgriPlex Genomics. The chromosome region at 2.130-2.292 Mb on D03 was significantly correlated with both SVD and RVD but not with MR. Based on the two most significant SNP markers, accessions homozygous for AA or TT SNP genotype averaged significantly lower SVD (0.88 vs. 2.54) and RVD (1.46 vs. 3.02) than those homozygous for CC or GG SNP genotype. The results suggested that a gene or genes within the region conferred resistance to vascular discoloration caused by FOV4. The Chinese Upland accessions had 37.22% homozygous AA or TT SNP genotype and 11.66% heterozygous AC or TG SNP genotype, while 32 US elite public breeding lines all had the CC or GG SNP genotype. Among 463 obsolete US Upland accessions, only 0.86% possessed the AA or TT SNP genotype. This study, for the first time, has developed diagnostic SNPs for marker-assisted selection and identified FOV4-resistant Upland germplasms with the SNPs.

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Section: Resultsmentioning

confidence: 58%

Targeted development of diagnostic SNP markers for resistance to Fusarium wilt race 4 in Upland cotton (Gossypium hirsutum)

Zhang

Wheeler²,

Dever³

et al. 2023

Preprint

Self Cite

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“…The results suggested segregation of resistance to FOV4 in many germplasm accessions. Our previous evaluations of FOV4 resistance in a set of eight cultivars including FM 2334GLT, Acala 1517‐08, and Pima PHY 881 RF (with the same resistant source and genetic background as Pima PHY 841 RF) were highly correlated between the greenhouse and field tests on a genotype basis (Zhang, Zhu, Abdelraheem et al., 2022; Zhang, Zhu, Elkins‐Arce, Wheeler, Dever, Whitelock, Hake et al., 2022), although the field evaluation had much higher disease escapes (Zhang, Zhu, Elkins‐Arce, Wheeler, Dever., Whitelock, Wedegaertner et al., 2022). The results were consistent with the current hydroponic study.…”

Section: Resultsmentioning

confidence: 99%

Comparative analysis of infection process in Upland cottons differing in resistance to Fusarium wilt caused byFusarium oxysporumf. sp.vasinfectumrace 4

Abdelraheem

Cooke

Wheeler³

et al. 2023

Crop Science

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Fusarium wilt (Fusarium oxysporum f. sp. vasinfectum race 4, FOV4) in cotton is an early season soil-borne vascular disease including seedling wilt and death. Its infection process in Upland cotton is currently unknown. This study compared the infection process among three Upland cotton cultivars (Gossypium hirsutum) using a confocal microscope. Seedlings were grown in a hydroponic system to the two-true leaf stage and inoculated with a virulent local FOV4 isolate. Based on the infection process, the resistant cultivar FM 2334GLT was divided into resistant (R) and susceptible (S) groups. The susceptible PHY 725 RF and FM 2334GLT(S) had the highest numbers of conidia attached and germinated on the root surface, followed by susceptible Acala 1517-08. FM 2334GLT(R) had the lowest numbers. Hyphae penetration and colonization inside the root followed a similar trend. In PHY 725 RF, FM 2334GLT(S), and Acala 1517-08, hyphae started to penetrate into epidermal cells at 2 days post inoculation (dpi), spread in the cortex intercellularly and intracellularly at 3-5 dpi, and entered the xylem vessels at 7 dpi, followed by colonization at 11-15 dpi. FM 2334GLT(R) had the lowest numbers of penetrating hyphae inside the root, with no apparent hyphae observed in the xylem. This study demonstrated that FM 2334GLT(R) had delayed infection, reduced fungal growth and reproduction, and prevented the FOV4 fungus from invading the xylem. The resistance in FM 2334GLT(R) was similar to that in Pima PHY 841 RF but with fewer hyphae in the cortex, suggesting possible different resistant mechanisms. INTRODUCTIONFusarium wilt (FW) of cotton (Gossypium spp.), caused by the soilborne fungus Fusarium oxysporum f. sp. vasinfectum (FOV), represents a serious fungal disease in cotton that limits

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Targeted development of diagnostic SNP markers for resistance to Fusarium wilt race 4 in Upland cotton (Gossypium hirsutum)

Zhang

Wheeler²,

Dever³

et al. 2023

Mol Genet Genomics

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Efficiency of selection for resistance to Fusarium wilt race 4 in cotton when conducted in the field versus greenhouse

Cited by 5 publications

References 43 publications

Targeted development of diagnostic SNP markers for resistance to Fusarium wilt race 4 in Upland cotton (Gossypium hirsutum)

Targeted development of diagnostic SNP markers for resistance to Fusarium wilt race 4 in Upland cotton (Gossypium hirsutum)

Comparative analysis of infection process in Upland cottons differing in resistance to Fusarium wilt caused byFusarium oxysporumf. sp.vasinfectumrace 4

Targeted development of diagnostic SNP markers for resistance to Fusarium wilt race 4 in Upland cotton (Gossypium hirsutum)

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