Efficiency of sildenafil encapsulation in poloxamer micelles

Chunhachaichana, Charisopon; Srichana, Teerapol

doi:10.1080/01932691.2018.1518142

Cited by 11 publications

(6 citation statements)

References 31 publications

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“…The Au 3+ concentration was determined by measuring the characteristic absorption peak of HAuCl 4 at 314 nm in accordance with a previous report ( Figure S1 ) 48 . Furthermore, the gold crystallization and growth were characterized by analyzing the light scattering intensity of growth solution via a dynamic light scattering (DLS) analyzer 49 . The AuNPs produced from gold nucleation growth generated evident light scattering signals, whereas the gold growth solution containing chloric acid and HA does not.…”

Section: Resultsmentioning

confidence: 99%

Avoiding the self-nucleation interference: a pH-regulated gold in situ growth strategy to enable ultrasensitive immunochromatographic diagnostics

Duan

Huang

et al. 2022

Theranostics

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Background: Gold nanoparticle-based immunochromatographic assay (AuNP-ICA) has insufficient sensitivity due to its inherent colorimetric signal intensity and low capture efficiency of AuNPs. The metal in situ growth is a common strategy to enhance the sensitivity of AuNP-ICA due to its superior signal amplification potential and simple operation. However, the detection distortion caused by metal self-nucleation during the growth process can seriously affect the accuracy and reproducibility of the strips. Methods: We present a pH-regulated gold in situ growth (GISG) strategy to amplify the colorimetric signal and demonstrate its application in improving the performance of traditional AuNP-ICA. The controllable growth signal amplification is achieved by lowering the pH of the growth solution to weaken the reducibility of hydroxylamine (HA), thus urging the crystallization and growth of Au 3+ on the AuNP surface instead of free reduction and self-nucleation. In addition, the mechanism of pH regulation on HA reducibility is elucidated by introducing an electron-donating or electron-withdrawing group to affect the electron density of hydroxyl group. Results: The proposed GISG strategy shows improved sensitivity, low background, robust operation, and good reproducibility. The LOD values of the designed GISG-amplified AuNP-ICA are as low as 0.0198 ng mL -1 for hepatitis B surface antigen and 0.0125 ng mL -1 for HIV-1 capsid p24 antigen, which are lower by about 500- and 70-fold, respectively, than those of the unamplified AuNP-ICA. Conclusions: This method is extended to enable ultrasensitive and rapid diagnosis of viral infections, and has potential as a general signal amplification platform to redefine immunochromatographic diagnostics.

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Section: Resultsmentioning

confidence: 99%

Avoiding the self-nucleation interference: a pH-regulated gold in situ growth strategy to enable ultrasensitive immunochromatographic diagnostics

Duan

Huang

et al. 2022

Theranostics

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“…The 10 mg/mL concentration of EBT provided stable and small-sized micelles. The solubility of EBT was increased due to the integration into hydrophobic core of micelles [ 19 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

“…This increase in entrapment efficiency and particle count and decrease in particle size can be attributed to micelles. The mixed micelles of TPGS/poloxamer-188 were formed above their CMC [ 19 ]. The results suggested that micelles integrated sublingual films were successfully prepared with desired entrapment efficiency and particle size.…”

Section: Discussionmentioning

confidence: 99%

“…The rational selection of amphiphilic molecules can play significant role for development of intended drug delivery system [ 18 ]. The reason of selection of poloxamer-188 compared to other high-molecular-poloxamers (HMPs) is that it produces small size micelles and sufficient stability to micelles [ 19 , 20 ]. Further, Poloxamer-188 is less mucoadhesive than HMPs.…”

Section: Introductionmentioning

confidence: 99%

“…The hydrophilic and hydrophobic portion of copolymer has the ability to self-assemble and to impede P-glycoprotein transporter. For instance, the activity of sildenafil (SIL) was boosted through poloxamer-188 micelles encapsulation by the research team of Charisopon Chunhachaichana [ 19 ]. Additionally, poloxamer-188 was frequently used with other polymeric and amphiphilic molecules for the formation of micelles [ 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

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Poloxamer-188 and d-α-Tocopheryl Polyethylene Glycol Succinate (TPGS-1000) Mixed Micelles Integrated Orodispersible Sublingual Films to Improve Oral Bioavailability of Ebastine; In Vitro and In Vivo Characterization

et al. 2021

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Orodispersible sublingual films (OSFs) composed of hydrophilic polymers were loaded with poloxamer-188 and d-α-tocopheryl polyethylene glycol succinate (TPGS-1000) mixed micelles to improve the oral bioavailability of a poorly soluble drug, ebastine (EBT). Mixed micelles formed by thin-film hydration method were incorporated into orodispersible sublingual film, consisting of HPMC and glycerol, using solvent casting technique. The mixed micelles and films were thoroughly evaluated for physicochemical characterization (size, polydispersity index, zeta potential, entrapment efficiency, thickness, weight, surface pH studies, disintegration time, swelling indices, mechanical properties, FTIR, PXRD, DSC, SEM, AFM, in vitro drug release, in vivo bioavailability, and toxicological studies). The results showed that the average particle size of mixed micelles was 73 nm. The mean zeta potential and PDI of the optimal mixed micelles formulation were −26 mV and 0.16, respectively. Furthermore, the maximum entrapment efficiency 82% was attained. The film’s disintegration time was in the range of 28 to 102 s in aqueous media. The integrity of micelles was not affected upon incorporation in films. Importantly, the micelles-loaded films revealed rapid absorption, high permeability, and increased bioavailability of EBT as compared to the pure drug. The existence of ebastine loaded mixed micelles in the films enhanced the bioavailability about 2.18 folds as compared to pure drug. Further, the results evidently established in-vitro and in-vivo performance of bioavailability enhancement, biocompatibility, and good safety profile of micelles-loaded orodispersible EBT films. Finally, it was concluded that film loaded with poloxamer-188/TPGS-1000 mixed micelles could be an effective carrier system for enhancing the bioavailability of ebastine.

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Development and characterization of nanodispersion-based sildenafil pressurized metered-dose inhaler using combined small-angle X-ray scattering, dynamic light scattering, and impactors

Chunhachaichana

Sawatdee

Rugmai

et al. 2022

Journal of Drug Delivery Science and Technology

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Efficiency of sildenafil encapsulation in poloxamer micelles

Cited by 11 publications

References 31 publications

Avoiding the self-nucleation interference: a pH-regulated gold in situ growth strategy to enable ultrasensitive immunochromatographic diagnostics

Avoiding the self-nucleation interference: a pH-regulated gold in situ growth strategy to enable ultrasensitive immunochromatographic diagnostics

Poloxamer-188 and d-α-Tocopheryl Polyethylene Glycol Succinate (TPGS-1000) Mixed Micelles Integrated Orodispersible Sublingual Films to Improve Oral Bioavailability of Ebastine; In Vitro and In Vivo Characterization

Development and characterization of nanodispersion-based sildenafil pressurized metered-dose inhaler using combined small-angle X-ray scattering, dynamic light scattering, and impactors

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