Efficiency of SSR and PawS markers for evaluation of genetic polymorphism among red clover (Trifolium pratense L.) cultivars

Клименко, Ирина; Kostenko, Sergey; Мавлютов, Ю. М.; Shamustakimova, Anastasia

doi:10.30901/2227-8834-2020-3-100-109

Cited by 2 publications

(1 citation statement)

References 25 publications

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“…А.А. Климен-ко доказал, что традиционные методы селекции в сочетании с методами клонирования, получения полиплоидных форм и элиминации леталей подходят для создания открытой партенозиготической популяции тутового шелкопряда, оптимальной для решения задач шелководства [3][4][5][6][7]. В.А.…”

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Advantages of Using Parthenogenetic Clones of the Silkworm (Bombyx Mori L.) in Hybridization

Larina¹,

Aqilov

Daniyarov

2021

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The fierce competition in the global silk market encourages the development and application of new biotechnologies in sericulture, such as cloning. The term “clone” in Greek means “branch”. A clone is an offspring of a single organism propagated without fertilization. All cloned individuals are genetically identical and are copies of each other. Clones are obtained in different ways. In case of the silkworm, this is a parthenogenetic development, which is an unnatural way of reproduction for the silkworm. Thermoactivation of an unfertilized greene at t0=46 0C during 18, leads to inhibition of the reduction division of meiosis in the silkworm germ cells. As a result, the eggs remain with a diploid set of chromosomes and develop as zygotes. Since the female cells in the sex chromosomes of the silkworm are heterogametic, only female parthenogenetic clones develop from thermally activated eggs. This feature makes silkworm clones extremely attractive for creating 100% pure hybrids. As the sericulture globally is based on the production of hybrids of the first generation only, in order to use maximum heterosis, the accuracy of preparation of hybrids is becoming particularly important.

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Section: Introductionunclassified

Advantages of Using Parthenogenetic Clones of the Silkworm (Bombyx Mori L.) in Hybridization

Larina¹,

Aqilov

Daniyarov

2021

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Investigation of genetic polymorphism of Russian rape and turnip rape varieties using SSR and SRAP markers

et al. 2022

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Rapeseed (Brassica napus L.) and turnip rape (B. rapa L. subsp. campestris (L.)) are important agricultural plants widely used for food, fodder and technical purposes and as green manure. Over the past decades, a large number of perspective varieties that are being currently cultivated in every region of Russia have been developed. To increase the breeding efficiency and facilitate the seed production, modern molecular-genetic techniques should be introduced as means to estimate species and varietal diversity. The objective of the presented research study was to investigate DNA polymorphism of the rapeseed and turnip rape varieties developed at Federal Williams Research Center of Forage Production and Agroecology and detect informative markers for varietal identification and genetic certification. To genotype 18 gDNA samples, 42 and 25 combinations of respective SSR and SRAP primers were used. The results obtained demonstrate that SRAP markers were more effective for polymorphism analysis: 36 % of the tested markers revealed genetic polymorphism compared with only 16.7 % of microsatellite loci. Molecular markers to detect differences at interspecific and intervarietal levels have also been found. For the investigated set, such microsatellite loci as Na12A02, Ni2C12, Ni02-D08a, Ra02-E01, Ni03H07а and SRAP-marker combinations as F13-R9, Me4-R7, F11-Em2, F10-R7, F9-Em2 and F9-R8 proved to be informative. Application of the two marker techniques made it possible to detect a higher level of DNA polymorphism in plants of different types (spring and winter varieties) if compared against the intervarietal differences within a species or a group. According to Nei’s genetic diversity index, in the cluster of winter rapeseed, VIK 2 and Gorizont varieties had the longest genetic distance, and in the spring cluster, these were Novosel and Veles. A high level of similarity was found between Vikros and Bizon winter rapeseed varieties. The results obtained have a high practical value for varietal specification of seed material and genetic certification of rapeseed and turnip rape varieties.

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Efficiency of SSR and PawS markers for evaluation of genetic polymorphism among red clover (Trifolium pratense L.) cultivars

Cited by 2 publications

References 25 publications

Advantages of Using Parthenogenetic Clones of the Silkworm (Bombyx Mori L.) in Hybridization

Advantages of Using Parthenogenetic Clones of the Silkworm (Bombyx Mori L.) in Hybridization

Investigation of genetic polymorphism of Russian rape and turnip rape varieties using SSR and SRAP markers

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