Efficiency of superoxide anions in the inactivation of selected dehydrogenases

Rodacka, Aleksandra; Serafin, Eligiusz; Puchała, Mieczysław

doi:10.1016/j.radphyschem.2010.04.001

Cited by 18 publications

(8 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hyperglycemia enhances the reducing equivalents to electron transport chain (ETC) and the electrochemical potential across the inner mitochondrial membrane and hence increases superoxide production [ 22 ]. Superoxide inhibits glyceraldehyde phosphate dehydrogenase (GAPDH) either directly or indirectly through PARP mediated NADH + depletion [ 30 , 31 ]. Inhibition of GAPDH by ROS leads to accumulation of glycolytic intermediates upstream of this enzyme and redirected to initiate cellular pathways like AGE formation.…”

Section: Oxidative Stress and Mitotoxicity: Role In Neuronal Dysfumentioning

confidence: 99%

Neuroinflammation and Oxidative Stress in Diabetic Neuropathy: Futuristic Strategies Based on These Targets

Sandireddy

Yerra

Areti

et al. 2014

International Journal of Endocrinology

288

194

View full text Add to dashboard Cite

In Diabetes, the chronic hyperglycemia and associated complications affecting peripheral nerves are one of the most commonly occurring microvascular complications with an overall prevalence of 50–60%. Among the vascular complications of diabetes, diabetic neuropathy is the most painful and disabling, fatal complication affecting the quality of life in patients. Several theories of etiologies surfaced down the lane, amongst which the oxidative stress mediated damage in neurons and surrounding glial cell has gained attention as one of the vital mechanisms in the pathogenesis of neuropathy. Mitochondria induced ROS and other oxidants are responsible for altering the balance between oxidants and innate antioxidant defence of the body. Oxidative-nitrosative stress not only activates the major pathways namely, polyol pathway flux, advanced glycation end products formation, activation of protein kinase C, and overactivity of the hexosamine pathway, but also initiates and amplifies neuroinflammation. The cross talk between oxidative stress and inflammation is due to the activation of NF-κB and AP-1 and inhibition of Nrf2, peroxynitrite mediate endothelial dysfunction, altered NO levels, and macrophage migration. These all culminate in the production of proinflammatory cytokines which are responsible for nerve tissue damage and debilitating neuropathies. This review focuses on the relationship between oxidative stress and neuroinflammation in the development and progression of diabetic neuropathy.

show abstract

Section: Oxidative Stress and Mitotoxicity: Role In Neuronal Dysfumentioning

confidence: 99%

Neuroinflammation and Oxidative Stress in Diabetic Neuropathy: Futuristic Strategies Based on These Targets

Sandireddy

Yerra

Areti

et al. 2014

International Journal of Endocrinology

288

194

View full text Add to dashboard Cite

show abstract

“…Superoxide anion radical formed in one-electron oxidation reaction of catechol moiety, may undergo dismutation to H 2 O 2 . Both superoxide anion and H 2 O 2 directly oxidize thiol group in the active site of GAPDH, which in consequence may decrease the enzyme catalytic activity [ 9 , 10 ] ( Fig 11 ).…”

Section: Discussionmentioning

confidence: 99%

“…The activity of the GAPDH (2 μM) and LDH (2 μM) was determined spectrophotometrically by measuring the rate of reduction of NAD + to NADH (in case of GAPDH) or oxidation of NADH to NAD + (for LDH), what has been described in details previously [ 10 ]. The relative activity of enzymes incubating with various concentrations of piceatannol (0; 3.2; 16; 32; 64 μM) was expressed as a percentage of the activity of control (enzyme without piceatannol) and it was determined during 120 min of incubation.…”

Section: Methodsmentioning

confidence: 99%

“…It is now well established that besides the traditional role in a glucose metabolism, GAPDH is implicated in the regulation of cell proliferation, DNA repair, tRNA export, membrane fusion and transport, cytoskeletal dynamics, cell death and many other cellular processes [ 5 ]. Due to the presence of a reactive, nucleophylic cysteine residue in the active site (Cys149), this multifunctional enzyme is particularly susceptible to oxidative modifications not only by reactive oxygen and nitrogen species, but also by peptide and protein peroxides and hydroperoxides [ 6 – 10 ]. It has been demonstrated that this cysteine, to the largest extent, is involved in the formation of aggregates and oligomers during prolonged exposure of GAPDH to oxidative stress [ 11 – 14 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Functional consequences of piceatannol binding to glyceraldehyde-3-phosphate dehydrogenase

et al. 2018

Self Cite

View full text Add to dashboard Cite

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is one of the key redox-sensitive proteins whose activity is largely affected by oxidative modifications at its highly reactive cysteine residue in the enzyme’s active site (Cys149). Prolonged exposure to oxidative stress may cause, inter alia, the formation of intermolecular disulfide bonds leading to accumulation of GAPDH aggregates and ultimately to cell death. Recently these anomalies have been linked with the pathogenesis of Alzheimer’s disease. Novel evidences indicate that low molecular compounds may be effective inhibitors potentially preventing the GAPDH translocation to the nucleus, and inhibiting or slowing down its aggregation and oligomerization. Therefore, we decided to establish the ability of naturally occurring compound, piceatannol, to interact with GAPDH and to reveal its effect on functional properties and selected parameters of the dehydrogenase structure. The obtained data revealed that piceatannol binds to GAPDH. The ITC analysis indicated that one molecule of the tetrameric enzyme may bind up to 8 molecules of polyphenol (7.3 ± 0.9). Potential binding sites of piceatannol to the GAPDH molecule were analyzed using the Ligand Fit algorithm. Conducted analysis detected 11 ligand binding positions. We indicated that piceatannol decreases GAPDH activity. Detailed analysis allowed us to presume that this effect is due to piceatannol ability to assemble a covalent binding with nucleophilic cysteine residue (Cys149) which is directly involved in the catalytic reaction. Consequently, our studies strongly indicate that piceatannol would be an exceptional inhibitor thanks to its ability to break the aforementioned pathologic disulfide linkage, and therefore to inhibit GAPDH aggregation. We demonstrated that by binding with GAPDH piceatannol blocks cysteine residue and counteracts its oxidative modifications, that induce oligomerization and GAPDH aggregation.

show abstract

“…2015) by causing structural changes in active sites avoiding binding and catalysis (Rodacka et al . 2010), modifying the structure and composition of amino acids (Bubler et al . 2017; Khani et al .…”

Section: Microbial and Enzyme Inactivation Mechanism Using Cold Plasmamentioning

confidence: 99%

Cold plasma an emerging nonthermal technology for milk and milk products: A review

Rathod

Kahar²,

Ranveer

et al. 2021

Int J of Dairy Tech

View full text Add to dashboard Cite

Plasma, the fourth state of matter, is under wide evaluation for the preservation of highly perishable foods, including milk and milk products. Cold plasma (CP) techniques have been promoted as a novel nonthermal technology for the preservation of milk and milk products. Apart from maintaining the nutritive value, CP also inactivates microorganisms without any chances of developing resistance. Cold plasma was also found to deactivate enzymes that are responsible for browning (colour change) reactions and generation of an off flavour. This review will describe the action of CP and its effect on the nutritional quality of milk and milk products.

show abstract

Efficiency of superoxide anions in the inactivation of selected dehydrogenases

Cited by 18 publications

References 27 publications

Neuroinflammation and Oxidative Stress in Diabetic Neuropathy: Futuristic Strategies Based on These Targets

Neuroinflammation and Oxidative Stress in Diabetic Neuropathy: Futuristic Strategies Based on These Targets

Functional consequences of piceatannol binding to glyceraldehyde-3-phosphate dehydrogenase

Cold plasma an emerging nonthermal technology for milk and milk products: A review

Contact Info

Product

Resources

About