Efficiency of the process of meiosis in scrotal testes of healthy boars and unilateral abdominal cryptorchid boars

Pinart, E.; Sancho, S.; Briz, M.; Bonet, Sergi; Badía, E.

doi:10.1002/(sici)1096-9926(199910)60:4<209::aid-tera5>3.0.co;2-4

Cited by 9 publications

(15 citation statements)

References 33 publications

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“…Recent work has found that unilateral abdominal cryptorchidism induces partial arrest of spermatogenesis at spermatocyte and spermatid steps in the scrotal testis of post‐pubertal boars (Pinart et al ., 1998, 1999a,b). In the present study, the increased content of glucosyl conjugates in spermatocytes and spermatids suggests that abnormal ion transport could be implicated in the arrest; according to Santi et al .…”

Section: Discussionmentioning

confidence: 99%

“…Testicular samples were ®xed in Bouins, dehydrated in graded ethanols and embedded in paraf®n (Pinart et al, 1999a). Cross sections 5 lm thick were marked with 13 lectins conjugated with peroxidase (PNA, SBA, WGA, LTA, Con A, DBA, UEA-I, HPA, RCA-I, LFA, AAA, SNA and GNA).…”

Section: Sample Processingmentioning

confidence: 99%

“…The structural and ultrastructural features of the seminiferous epithelium in healthy boars, right-sided unilaterally cryptorchid boars and bilaterally cryptorchid boars was recently described (Pinart et al, 1999a(Pinart et al, ,b, 2000. In healthy boars, the seminiferous epithelium of the left and right testes had mature Sertoli cells and normal spermatogenesis (Pinart et al, 1999a. In the left (scrotal) testis of unilaterally cryptorchid boars, the seminiferous epithelium showed mature Sertoli cells but partial arrest of spermatogenesis at spermatocyte and spermatid stages (Pinart et al, 1999a,b,c), whereas in the right (abdominal) testis it was constituted by either immature Sertoli cells and a few degenerating spermatogonia or only immature Sertoli cells (Pinart et al, 1999a.…”

Section: Introductionmentioning

confidence: 99%

“…In the left (abdominal) testis of bilaterally cryptorchid boars, the seminiferous epithelium contained either immature Sertoli cells and a few spermatogonia or only immature Sertoli cells; both immature Sertoli cells and spermatogonia displayed degenerative signs (Pinart et al, 1999a. The right (abdominal) testes of bilaterally cryptorchid boars had few degenerated seminiferous tubules constituted by remnants of immature Sertoli cells (Pinart et al, 1999a).…”

Section: Introductionmentioning

confidence: 99%

“…Lectin histochemistry is considered a valuable method for determining changes in the glycoconjugate content during spermatogenesis under normal and pathological conditions (Wine & Chapin, 1997;Arenas et al, 1998). Additionally, the study of cryptorchidism in boars is of great interest because the results can be extrapolated to humans (Pinart et al, 1999a(Pinart et al, ,c, 2000. Recent data pointed out the importance of studying the post-puberal cryptorchidism in order to better understand the pathogenesis of reduced fertility in treated and untreated adult cryptorchid males (Pinart et al, 1999b.…”

Section: Introductionmentioning

confidence: 99%

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Lectin affinity of the seminiferous epithelium in healthy and cryptorchid post‐pubertal boars

et al. 2001

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The present study describes the sugar content of the seminiferous epithelium, using lectin histochemistry, in healthy boars and in boars with unilateral and bilateral abdominal cryptorchidism. In healthy boars the apical cytoplasm of Sertoli cells exhibited abundant glucosyl (Con A and WGA lectins), galactosyl (HPA, DBA, SBA and PNA lectins), and fucosyl (AAA lectin) residues. Spermatogonia and spermatocytes contained abundant glucosyl (Con A and WGA lectins) and fucosyl (AAA lectin) residues. In spermatids, galactosyl (SBA and PNA lectins) and glucosyl (Con A and WGA lectins) residues increased progressively throughout spermiogenesis, and fucosyl (AAA lectin) residues decreased. As compared with healthy boars, the scrotal testis of unilateral cryptorchid boars showed decreased amounts of fucosyl (AAA lectin) and galactosyl (HPA and DBA lectins) residues on the Sertoli cell apical cytoplasm; spermatocytes exhibited higher content of glucosyl (Con A lectin) residues and spermatids showed altered nature of glucosyl (Con A and WGA lectins) and galactosyl (SBA and PNA lectins) complexes. In abdominal testes of unilateral and bilateral cryptorchid boars, immature Sertoli cells and spermatogonia showed decreased fucosyl (AAA lectin), and increased glucosyl (Con A and WGA lectins) and galactosyl (SBA and PNA lectins) contents. These results suggest that the seminiferous epithelium of healthy boars has polarized activity with the apical compartment implicated in germ cell-Sertoli cell adhesion and interaction, in transport of ions, substrates and fluids, and in acrosomal differentiation. In scrotal testes, unilateral abdominal cryptorchidism could lead to defective germ cell-Sertoli cell adhesion, impaired acrosomal differentiation and increased ionic transport in the apical compartment of the seminiferous epithelium. Unilateral and bilateral cryptorchidism could induce increased ionic transport and membrane permeability in the seminiferous epithelium of abdominal testes.

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Section: Discussionmentioning

confidence: 99%

Section: Sample Processingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Lectin affinity of the seminiferous epithelium in healthy and cryptorchid post‐pubertal boars

et al. 2001

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Morphologic and histochemical study of blood capillaries in boar testes: Effects of abdominal cryptorchidism

et al. 2001

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Background Few data exist about the features of testicular microvasculature under normal and pathologic conditions. Methods The morphology and lectin affinity of testicular capillaries were examined in healthy boars and in unilateral and bilateral abdominal cryptorchid boars. Results The capillaries of scrotal testes contained a) the endothelial layer formed by two cells, b) the basal lamina constituted by collagen fibers and glycoconjugates with fucosyl, galactosyl, glucosyl, and neuraminic acid residues, and c) the pericyte layer formed by a single cell. These components participated in substrate exchange between blood and testicular tissue. The abdominal testes showed increased numbers of capillaries, which could exhibit a mature appearance, but also angiogenic or degenerative patterns. Angiogenesis was manifested in interstitial capillaries and was characterized by a) proliferation of endothelial cells, b) decreased thickness and decreased content of collagen fibers and glycoconjugates in the basal lamina, and c) lack of pericytes. Degenerative capillaries lay in association with seminiferous tubules and showed a) pyknotic endothelial cells; b) thickening, collagenization, and altered glycoconjugate content in the basal lamina; and c) increased development of pericytes. The angiogenesis of interstitial capillaries resulted in high vascular permeability, and the degeneration of intertubular capillaries led to defective substrate exchange between blood and seminiferous tubules. Conclusions Unilateral abdominal cryptorchidism did not alter the morphology and function of capillaries in the scrotal testis. Unilateral and bilateral abdominal cryptorchidism resulted in increased numbers and abnormal morphology and function of capillaries in abdominal testes. The proliferation of interstitial capillaries correlated with the immaturity of Leydig cells, and the degeneration of intertubular capillaries correlated with the thickening of the lamina propria. Teratology 63:42–51, 2001. © 2001 Wiley‐Liss, Inc.

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The Boar Reproductive System

2013

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Efficiency of the process of meiosis in scrotal testes of healthy boars and unilateral abdominal cryptorchid boars

Cited by 9 publications

References 33 publications

Lectin affinity of the seminiferous epithelium in healthy and cryptorchid post‐pubertal boars

Lectin affinity of the seminiferous epithelium in healthy and cryptorchid post‐pubertal boars

Morphologic and histochemical study of blood capillaries in boar testes: Effects of abdominal cryptorchidism

The Boar Reproductive System

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