Efficient (3R)-Acetoin Production from meso-2,3-Butanediol Using a New Whole-Cell Biocatalyst with Co-Expression of meso-2,3-Butanediol Dehydrogenase, NADH Oxidase, and Vitreoscilla Hemoglobin

Abstract

Acetoin (AC) is a volatile platform compound with various potential industrial applications. AC contains two stereoisomeric forms: (3)-AC and (3)-AC. Optically pure AC is an important potential intermediate and widely used as a precursor to synthesize novel optically active materials. In this study, chiral (3)-AC production from -2,3-butanediol (-2,3-BD) was obtained using recombinant cells co-expressing-2,3-butanediol dehydrogenase (-2,3-BDH), NADH oxidase (NOX), and hemoglobin protein (VHB) from sp. T241,, and , respectively. The new biocatalyst of/pET--- was developed and the bioconversion conditions were optimized. Under the optimal conditions, 86.74 g/l of (3)-AC with the productivity of 3.61 g/l/h and the stereoisomeric purity of 97.89% was achieved from 93.73 g/l -2,3-BD using the whole-cell biocatalyst. The yield and productivity were new records for (3)-AC production. The results exhibit the industrial potential for (3)-AC production via whole-cell biocatalysis.

N-Acyl-Homoserine Lactone Quorum Sensing Switch from Acidogenesis to Solventogenesis during the Fermentation Process in Serratia marcescens MG1

“…Another important property of S. marcescens MG1 is that it is capable of producing a large number of neutral compounds (instead of mixed acids), such as acetoin and 2,3-butanediol (two important biobased bulk chemicals [10,11]), when cultured under fermentation conditions using carbohydrates as a carbon source. To date, the physiological function of 2,3-butanediol has not been clarified.…”

Simultaneous biosynthesis of (R)-acetoin and ethylene glycol from D-xylose through in vitro metabolic engineering

“…In addition to acetoin or EG, the pyruvate generated can be subsequently channelled into other products, including 2,3-butanediol, acetate, lactate and ethanol, leading to extra recovery processes of products from fermented broths ( Xiao and Lu, 2014b ). Alternatively, enzyme or whole-cell biocatalysis for producing enantiomerically pure acetoin and EG production could take advantage of stereo-selectivity and high catalytic efficiency ( Guo et al, 2016 ). As such, biocatalytic routes were reported for ( R )-acetoin synthesis using the acetoin analogue 2,3-butanediol ( Guo et al, 2016 , Kochius et al, 2014 , Xiao et al, 2010 ) or diacetyl ( Heidlas and Tressl, 1990 , Yu et al, 2015 ), as the precursor.…”

“…Alternatively, enzyme or whole-cell biocatalysis for producing enantiomerically pure acetoin and EG production could take advantage of stereo-selectivity and high catalytic efficiency ( Guo et al, 2016 ). As such, biocatalytic routes were reported for ( R )-acetoin synthesis using the acetoin analogue 2,3-butanediol ( Guo et al, 2016 , Kochius et al, 2014 , Xiao et al, 2010 ) or diacetyl ( Heidlas and Tressl, 1990 , Yu et al, 2015 ), as the precursor. However, biocatalytic routes have not been reported for production of ( R )-acetoin and EG using monosaccharides as starting materials.…”

Efficient (3S)-Acetoin and (2S,3S)-2,3-Butanediol Production from meso-2,3-Butanediol Using Whole-Cell Biocatalysis

“…Its precursor, acetoin (AC), is one of the 30 platform chemicals which are given priority regarding their development and utilization by the United States Department of Energy [ 3 , 4 ]. 2,3-Butanediol has three stereoisomers ((2 S ,3 S )-2,3-butanediol, (2 R ,3 R )-2,3-butanediol, and meso -2,3-butanediol), while acetoin has two stereoisomers, including (3 R )-acetoin and (3 S )-acetoin [ 5 , 6 , 7 , 8 ]. These isomers of 2,3-butanediol and acetoin showed important potential for synthesis of pharmaceutical intermediates [ 6 , 7 ].…”