Efficient accumulation of oleic acid in Saccharomyces cerevisiae caused by expression of rat elongase 2 gene (rELO2) and its contribution to tolerance to alcohols

Yazawa, Hisashi; Kamisaka, Yasushi; Kimura, Kouji; Yamaoka, Masakazu; Uemura, Hiroshi

doi:10.1007/s00253-011-3410-4

Cited by 30 publications

(23 citation statements)

References 34 publications

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“…43,44 As shown in Table 2, there were 7 DEGs with related to fatty acid metabolic process, and 3 DEGs with related to the ergosterol biosynthetic process, and almost all of them were down-regulated except LipA (KMAR_50026) coding lipoyl synthase. Among those down-regulated genes, OLE1 encodes acylCoA desaturase 1 involved in desaturation of fatty acid and with greatest transcriptional abundance ( Table 2).…”

Section: Degs Related To Fatty Acid and Ergosterol Metabolismmentioning

confidence: 99%

Transcriptomic analysis of thermotolerant yeastKluyveromyces marxianusin multiple inhibitors tolerance

Wang

Yang

et al. 2018

RSC Adv.

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During pretreatment of lignocellulosic biomass, toxic compounds were released and inhibited the growth and fermentation of microorganisms. Here the global transcriptional response of K. marxianus to multiple inhibitors including acetic acid, phenols, furfural and HMF, at 42 C, was studied, via RNA-seq technology.Genes involved in the glycolysis pathway, fatty acid metabolism, ergosterol metabolism and vitamin B6 and B1 metabolic process were enriched in the down-regulated gene set, while genes involved in TCA cycle, respiratory chain, ROS detoxification and transporter coding genes were enriched in the up-regulated gene set in response to the multiple inhibitors stress. Further real time-PCR results with three single inhibitor stress conditions showed that different transporters responded quite differently to different inhibitor stress. Coenzyme assay results showed that the level of NAD + was increased and both NADH/ NAD + and NADPH/NADP + ratio decreased. Furthermore, genes involved with transcription factors related to carbohydrate metabolism, sulfur amino acids metabolism, lipid metabolism or those directly involved in the transcriptional process were significantly regulated. Though belonging to different GO categories or KEGG pathway, many differentially expressed genes were enriched in maintaining the redox balance, NAD(P) + /NAD(P)H homeostasis or NAD + synthesis, energy production, and iron transportation or metabolism. These results suggest that engineering these aspects represents a possible strategy to develop more robust strains for industrial fermentation from cellulosic biomass.

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Section: Degs Related To Fatty Acid and Ergosterol Metabolismmentioning

confidence: 99%

Transcriptomic analysis of thermotolerant yeastKluyveromyces marxianusin multiple inhibitors tolerance

Wang

Yang

et al. 2018

RSC Adv.

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“…Modification of yeast sterol or fatty acid composition has led to differences in growth rates and has also altered susceptibility to drugs or ethanol as a result of changes in membrane fluidity (7)(8)(9)(10). Of various groups of candidates evaluated as agents for selection of higher bisabolene levels in S. cerevisiae, nonionic surfactants were found to be the most promising.…”

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confidence: 99%

Use of Nonionic Surfactants for Improvement of Terpene Production in Saccharomyces cerevisiae

Kirby

Nishimoto

Chow

et al. 2014

Appl Environ Microbiol

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dTo facilitate enzyme and pathway engineering, a selection was developed for improved sesquiterpene titers in Saccharomyces cerevisiae. ␣-Bisabolene, a candidate advanced biofuel, was found to protect yeast against the disruptive action of nonionic surfactants such as Tween 20 (T20). An experiment employing competition between two strains of yeast, one of which makes twice as much bisabolene as the other, demonstrated that growth in the presence of T20 provided sufficient selective pressure to enrich the high-titer strain to form 97% of the population. Following this, various methods were used to mutagenize the bisabolene synthase (BIS) coding sequence, coupled with selection by subculturing in the presence of T20. Mutagenesis targeting the BIS active site did not yield an improvement in bisabolene titers, although mutants were found which made a mixture of ␣-bisabolene and ␤-farnesene, another candidate biofuel. Based on evidence that the 3= end of the BIS mRNA may be unstable in yeast, we randomly recoded the last 20 amino acids of the enzyme and, following selection in T20, found a variant which increased specific production of bisabolene by more than 30%. Since T20 could enrich a mixed population, efficiently removing strains that produced little or no bisabolene, we investigated whether it could also be applied to sustain high product titers in a monoculture for an extended period. Cultures grown in the presence of T20 for 14 days produced bisabolene at titers up to 4-fold higher than cultures grown with an overlay of dodecane, used to sequester the terpene product, and 20-fold higher than cultures grown without dodecane.

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“…Rather than the first reports suggesting the importance of unsaturation but not of its specificity, the most recent reports point to oleic acid (C18:ln-9) but not palmitoleic acid (C16:1n-7) as important for ethanol tolerance (You et al 2003; reviewed by Ma and Liu 2010). Genome-wide screening of ethanol tolerant mutants also suggested that the increase in oleic acid content was the factor in ethanol tolerance as well as the enhancement of the cell wall integrity (Yazawa et al 2007b), and it was confirmed that the content of oleic acid increased as the initial concentration of ethanol in media increased (Yazawa et al 2011).…”

Section: Ethanol Tolerancementioning

confidence: 80%

“…To clarify the importance of oleic acid, and to create an alcohol stress tolerant S. cerevisiae by artificially altering the fatty acid composition of the cells instead of searching and isolating mutant strains, Yazawa et al (2011) have attempted to make the content of oleic acid predominant even under the normal growth condition by introducing rat elongase genes that elongate C 16 fatty acids to C 18 . Rat has two elongase genes (rELO1 and rELO2): rELO1 catalyzes the elongation of mono-and polyunsaturated fatty acids of C 16 -C 20 size, while rELO2 mainly catalyzes elongation of C16:0 to C18:0 (Inagaki et al 2002).…”

Section: Ethanol Tolerancementioning

confidence: 99%

Synthesis and production of unsaturated and polyunsaturated fatty acids in yeast: current state and perspectives

Uemura

2012

Appl Microbiol Biotechnol

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Recently, many genes involved in the formation of unsaturated and polyunsaturated fatty acids (PUFAs) were isolated. In most cases, their activities were confirmed by expressing them in the well-studied model organism Saccharomyces cerevisiae because its fatty acid compositions are very simple and it does not contain PUFAs. Taking advantage of its genetic tractability and increasing wealth of accessible data, many groups are attempting to produce various useful fatty acids in the model yeasts, mainly in S. cerevisiae. This review describes typical such examples including a very recent study on the expression of a fatty acid hydroxylase gene in fission yeast Schizosaccharomyces pombe. Furthermore, the impact of the genetically engineered alteration of fatty acid composition on the stress tolerance is presented because unsaturated fatty acids have crucial roles in membrane fluidity and signaling processes. Lastly, recent attempts at increasing lipid content in S. cerevisiae are discussed.

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Efficient accumulation of oleic acid in Saccharomyces cerevisiae caused by expression of rat elongase 2 gene (rELO2) and its contribution to tolerance to alcohols

Cited by 30 publications

References 34 publications

Transcriptomic analysis of thermotolerant yeastKluyveromyces marxianusin multiple inhibitors tolerance

Transcriptomic analysis of thermotolerant yeastKluyveromyces marxianusin multiple inhibitors tolerance

Use of Nonionic Surfactants for Improvement of Terpene Production in Saccharomyces cerevisiae

Synthesis and production of unsaturated and polyunsaturated fatty acids in yeast: current state and perspectives

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