Efficient acetylcholinesterase immobilization for improved electrochemical performance in polypyrrole nanocomposite-based biosensors for carbaryl pesticide

Loguercio, Lara Fernandes; Thesing, Anderson; Demingos, Pedro Guerra; Albuquerque, Carlos Diego Lima de; Rodrigues, Roberta S.B.; Brolo, Alexandre G.; Santos, Jacqueline Ferreira Leite

doi:10.1016/j.snb.2021.129875

Cited by 48 publications

(14 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…By using the slope of the calibration curve/electroactive surface area ratio, the sensitivity was 0.03748 μA L μmol −1 cm −2 , and the LOD was 1.0 μmol cm 2 L −1 [64,65] …”

Section: Resultsmentioning

confidence: 99%

“…By using the slope of the calibration curve/electroactive surface area ratio, the sensitivity was 0.03748 μA L μmol À 1 cm À 2 , and the LOD was 1.0 μmol cm 2 L À 1 . [64,65] We proposed that the good interaction of PFA/GO-3.88 W for AA can be ascribed to the increase in the density of electronic states at the edge plane of electrodes; i. e. oxygenated groups such as carboxylic acids, resulting in innumerable catalytic centers that can effectively catalyze the oxidation of AA (Figure 4 and Table S1). The presence of aromatic carbonbased materials increases the p À p stacking interactions with AA molecule onto the electrode.…”

Section: Determination Of Ascorbic Acidmentioning

confidence: 99%

See 1 more Smart Citation

Direct Laser Writing of Poly(furfuryl Alcohol)/Graphene Oxide Electrodes for Electrochemical Determination of Ascorbic Acid

et al. 2022

Self Cite

View full text Add to dashboard Cite

Due to the considerable importance of preventing and treating diseases, efficient detection methods are required to monitor levels of ascorbic acid (AA) in beverages, foods, dietary supplements, and biological fluids. In this work, an efficient, easy handling, low cost, and simple fabrication process for non‐enzymatic electrochemical sensors was fabricated through the carbonization of a graphene oxide filled biomass‐derived polymer poly(furfuryl alcohol) (PFA/GO), as sustainable alternative, using a high throughput CO2 laser‐scribing process. The laser power was found to determine the physicochemical properties of the resulting graphene‐like electrodes. As an electrochemical sensor, devices presented a detection limit of 1.0 μmol cm2 L−1 with good reproducibility towards AA oxidation. For real sample measurements, recovery rates between 97 and 113 % were found in commercial vitamin‐C tablet. Analysis of AA in synthetic sweat presented good intra‐electrode reproducibility and limit of detection of 1.3 μmol cm2 L−1.

show abstract

“…By using the slope of the calibration curve/electroactive surface area ratio, the sensitivity was 0.03748 μA L μmol −1 cm −2 , and the LOD was 1.0 μmol cm 2 L −1 [64,65] …”

Section: Resultsmentioning

confidence: 99%

Section: Determination Of Ascorbic Acidmentioning

confidence: 99%

Direct Laser Writing of Poly(furfuryl Alcohol)/Graphene Oxide Electrodes for Electrochemical Determination of Ascorbic Acid

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…The biosensor based on an electropolymerization polyaniline film for immobilization of the AChE enzyme in carbon nanotubes was developed by Cesarino et al [ 43 ]. Loguercio et al [ 44 ] optimized the surface conditions for the immobilization of the AChE enzyme using indigo carmine and dodecyl sulfate doped polypyrrole—gold nanoparticle nanocomposite films. In addition, the AChE-e-pGON/GCE and GC/rGO/AChE biosensors, developed, respectively, by Li et al [ 45 ] and Silva et al [ 7 ], which stood out among the biosensors with lower LODs for detecting the carbaryl pesticide.…”

Section: Resultsmentioning

confidence: 99%

Acetylcholinesterase Biosensor Based on Functionalized Renewable Carbon Platform for Detection of Carbaryl in Food

et al. 2022

View full text Add to dashboard Cite

Enzymatic electrochemical biosensors play an important role in the agri-food sector due to the need to develop sustainable, low-cost, and easy-to-use analytical devices. Such biosensors can be used to monitor pathogens, endocrine disruptors, and pesticides, such as carbaryl, widely used in many crops. The use of renewable carbon (RC) sources, provided from biomass pyrolysis has been often applied in the fabrication of such sensors. This material is a great candidate for biosensor fabrication due to the presence of surface functional groups, porosity, and moderate surface area. This work describes the functionalization of RC material through an acid treatment with a sulfonitric solution HNO3/H2SO4 (1:3) and the resulting material was characterized by scanning electron microscopy. The obtained RC functionalized (RCF) and the acetylcholinesterase enzyme (AChE) were applied in the construction of the electrochemical biosensor on glassy carbon (GC) electrode and used to detect carbaryl in apple samples. The GC/RCF/AChE biosensor was able to detect the carbaryl pesticide from 5.0 to 30.0 nmol L−1, displaying a LOD of 4.5 nmol L−1. The detection of carbaryl in apple samples presented recoveries between 102.5 to 118.6% through the standard addition method. The proposed biosensor is a promising renewable tool for food safety.

show abstract

“…Nowadays, acetylcholinesterase (AChE) inhibition-based electrochemical biosensors have attracted great attention with regard to the detection of carbaryl and other carbamate pesticides due to their advantages of non-toxicity, simplicity, miniaturized, high specificity and high sensitivity [ 21 , 22 , 23 ]. For example, Loguercio et al established the biosensor for the detection of carbaryl by immobilizing AChE on the polypyrrole nanocomposite, showing satisfactory results [ 24 ]. Zhang et al used graphene as the support material to load AChE, and the constructed sensor realized the chiral recognition of (+)/(−)-methamidophos [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Biosensor Based on Covalent Organic Framework Immobilized Acetylcholinesterase for Ratiometric Detection of Carbaryl

Luo

Wang

et al. 2022

Biosensors

View full text Add to dashboard Cite

A ratiometric electrochemical biosensor based on a covalent organic framework (COFThi-TFPB) loaded with acetylcholinesterase (AChE) was developed. First, an electroactive COFThi-TFPB with a two-dimensional sheet structure, positive charge and a pair of inert redox peaks was synthesized via a dehydration condensation reaction between positively charged thionine (Thi) and 1,3,5-triformylphenylbenzene (TFPB). The immobilization of AChE on the positively charged electrode surface was beneficial for maintaining its bioactivity and achieving the best catalytic effect; therefore, the positively charged COFThi-TFPB was an appropriate support material for AChE. Furthermore, the COFThi-TFPB provided a stable internal reference signal for the constructed AChE inhibition-based electrochemical biosensor to eliminate various effects which were unrelated to the detection of carbaryl. The sensor had a linear range of 2.2–60 μM with a detection limit of 0.22 μM, and exhibited satisfactory reproducibility, stability and anti-interference ability for the detection of carbaryl. This work offers a possibility for the application of COF-based materials in the detection of low-level pesticide residues.

show abstract

Efficient acetylcholinesterase immobilization for improved electrochemical performance in polypyrrole nanocomposite-based biosensors for carbaryl pesticide

Cited by 48 publications

References 70 publications

Direct Laser Writing of Poly(furfuryl Alcohol)/Graphene Oxide Electrodes for Electrochemical Determination of Ascorbic Acid

Direct Laser Writing of Poly(furfuryl Alcohol)/Graphene Oxide Electrodes for Electrochemical Determination of Ascorbic Acid

Acetylcholinesterase Biosensor Based on Functionalized Renewable Carbon Platform for Detection of Carbaryl in Food

Biosensor Based on Covalent Organic Framework Immobilized Acetylcholinesterase for Ratiometric Detection of Carbaryl

Contact Info

Product

Resources

About