Efficient adventitial gene delivery to rabbit carotid artery with cationic polymer–plasmid complexes

Turunen, Mikko P.; Hiltunen, Mikko; Ruponen, Marika; Virkamäki, L; Szoka, F; Urtti, Arto; Ylä‐Herttuala, Seppo

doi:10.1038/sj.gt.3300800

Cited by 140 publications

(99 citation statements)

References 27 publications

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“…30 In addition, the transfection efficiency of the dendrimer was evaluated to smooth muscle cells and endothelial cells. 27 The dendrimer was effective in that it had higher transfection efficiency than naked pDNA and prolonged gene expression. However, as proved in our study, the dendrimer (SuperFect) is highly toxic to smooth muscle cells, suggesting that WSLP has an advantage against the dendrimer in terms of safety.…”

Section: Discussionmentioning

confidence: 99%

“…PEI25 000/ pCMV-Luc complex was prepared at a 5/1 N/P ratio, based on the previous reports. 22,[26][27][28][29] After transfection to A7R5 cells, transgene expression was evaluated by luciferase assay. As a result, WSLP showed higher transfection efficiency than PEI1800, SuperFect, or lipofectamine to A7R5 cells ( Figure 4).…”

Section: In Vitro Transfection Assay Of Wslp To Smooth Muscle Cellsmentioning

confidence: 99%

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Water-soluble lipopolymer as an efficient carrier for gene delivery to myocardium

et al. 2003

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Water-soluble lipopolymer (WSLP), which consisted of polyethylenimine (PEI, 1800 Da) and cholesterol, was characterized as a gene carrier to smooth muscle cells and myocardium. Acid-base titration showed that WSLP had a proton-buffering effect. The size of WSLP/plasmid DNA (pDNA) complex was around 70 nm. WSLP/pDNA complex was transfected to A7R5 cells, a smooth muscle cell line. WSLP showed the highest transfection at a 40/1 N/P ratio. WSLP has higher transfection efficiency than PEI (1800 and 25 000 Da), SuperFect, and lipofectamine. In addition, WSLP has less cytotoxicity than PEI (25 000 Da), SuperFect, and lipofectamine. Since WSLP has cholesterol moiety, it may utilize cellular cholesterol uptake pathway, in which lowdensity lipoprotein (LDL) is involved. An inhibition study with free cholesterol or low-density lipoprotein (LDL) showed that transfection was inhibited by cholesterol or LDL, suggesting that WSLP/pDNA complex is transfected to the cells through the cholesterol uptake pathway. To evaluate the transfection efficiency to myocardium, WSLP/pDNA complex was injected into the rabbit myocardium. WSLP showed higher transfection than PEI and naked pDNA. WSLP expressed the transgene for more than 2 weeks. In conclusion, WSLP is an efficient carrier for local gene transfection to myocardium, and useful in in vivo gene therapy.

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Section: Discussionmentioning

confidence: 99%

Section: In Vitro Transfection Assay Of Wslp To Smooth Muscle Cellsmentioning

confidence: 99%

Water-soluble lipopolymer as an efficient carrier for gene delivery to myocardium

et al. 2003

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“…22 In contrast, several reports indicate that low molecular weight PEI (25 kDa) at a charge ratio of 4:1-6:1 (+/−) is more effective than PEI (800 kDa) in vivo. 23,24 Therefore, all in vivo comparisons were performed against the low molecular weight PEI at a charge ratio of 5:1 (+/−). 25 In vitro studies: In order to investigate whether UPC protects the pDNA against degradation by endogenous enzymes, the charge-dependent stability and transgene expression in 293 cells were investigated in the presence of serum.…”

Section: Comparison Of Upc and Pei Polyplexesmentioning

confidence: 99%

Chitosan as a nonviral gene delivery system. Structure–property relationships and characteristics compared with polyethylenimine in vitro and after lung administration in vivo

et al. 2001

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Chitosan is a natural cationic linear polymer that has recently emerged as an alternative nonviral gene delivery system. We have established the relationships between the structure and the properties of chitosan-pDNA polyplexes in vitro. Further, we have compared polyplexes of ultrapure chitosan (UPC) of preferred molecular structure with those of optimised polyethylenimine (PEI) polyplexes in vitro and after intratracheal administration to mice in vivo. Chitosans in which over two out of three monomer units carried a primary amino group formed stable colloidal polyplexes with pDNA. Optimized UPC and PEI polyplexes protected the pDNA from serum degradation to approximately the same degree, and they gave a comparable maximal transgene expression in 293 cells. In contrast to PEI, UPC was non toxic at escalating doses. After intratracheal administration,

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“…Following 2 h incubation at 371C, 5% CO 2 , 5 mM of sodium butyrate was added to all but HepG2 cells, which had 2.5 mM of sodium butyrate. After 48 h incubation, cells were fixed with 1.25% glutaraldehyde, stained with X-gal to visualise b-galactosidaseexpressing cells 33 and blue cells were counted.…”

Section: Transduction Experimentsmentioning

confidence: 99%

Truncated vesicular stomatitis virus G protein improves baculovirus transduction efficiency in vitro and in vivo

et al. 2005

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Efficient adventitial gene delivery to rabbit carotid artery with cationic polymer–plasmid complexes

Cited by 140 publications

References 27 publications

Water-soluble lipopolymer as an efficient carrier for gene delivery to myocardium

Water-soluble lipopolymer as an efficient carrier for gene delivery to myocardium

Chitosan as a nonviral gene delivery system. Structure–property relationships and characteristics compared with polyethylenimine in vitro and after lung administration in vivo

Truncated vesicular stomatitis virus G protein improves baculovirus transduction efficiency in vitro and in vivo

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