Efficient and low-cost alternative of lipase concentration aiming at the application in the treatment of waste cooking oils

Preczeski, Karina Paula; Kamanski, Angela B.; Scapini, Thamarys; Camargo, Aline Frumi; Modkoski, Tatiani A.; Rossetto, Vanusa; Venturin, Bruno; Mulinari, Jéssica; Golunski, Simone Maria; Mossi, Altemir José; Treichel, Helen

doi:10.1007/s00449-018-1919-y

Cited by 18 publications

(9 citation statements)

References 26 publications

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“…The reactions were prepared with 0.5 mol L −1 salt concentration and 50% (v v −1 ) solvent concentration and dripped into the enzyme extract at a flow rate of 10 mL min −1 . The assays were conducted at 4 • C. After precipitation, the volumes were centrifuged at 9,000 × g for 20 min at 4 • C and the pellets were resuspended in 50 mM Tris HCl (pH 8.5) buffer (Farag and Hassan, 2004;Preczeski et al, 2018). Keratinolytic activity was measured in both supernatants and pellets.…”

Section: Enzymatic Precipitationmentioning

confidence: 99%

“…The specific activities of these enzymes may be increased using enzymatic concentration techniques such as precipitation. This methodology uses low-cost reagents to separate enzymes from interferents (Sala et al, 2014;Preczeski et al, 2018). Keratinases, both crude and concentrated, have several applications, especially in the environmental area (Cervantes-González et al, 2009;Paul et al, 2014;Bhange et al, 2015;Reddy et al, 2017;Su et al, 2017;Abdel-Fattah et al, 2018;Choińska-Pulit et al, 2019;Kalaikumari et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Fusarium oxysporum and Aspergillus sp. as Keratinase Producers Using Swine Hair From Agroindustrial Residues

Preczeski

Dalastra

Czapela

et al. 2020

Front. Bioeng. Biotechnol.

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Technological processes mediated by microorganisms and enzymes are promising alternatives for treatment of recalcitrant residues. Keratinases hydrolyze keratin, the primary component of some wastes generated in many industrial activities. The present study was designed to evaluate strategies for obtaining keratinases produced by fungi using submerged fermentation and two residues as substrates, chicken feathers and swine hair. Two fungi isolated from feather residues showed potential for keratinase production, Fusarium oxysporum and Aspergillus sp. These were subjected to submerged fermentation using chicken feathers and swine hair prepared in three conditions (microbial concentration reduction, sterilization and hydrogen peroxide). The residual mass was quantified and tested for keratinase production. The most potent enzymatic extract was used in the precipitation technique with salts and organic solvents. The best results of enzymatic activity were obtained using F. oxysporum, on the 6thday of fermentation, obtaining 243.25 U mL −1 using sterilized swine hair as the substrate. Aspergillus sp. showed the highest keratinolytic activity on the 9thday, 113.50 U mL −1 using feathers as the substrate. The highest degradation percentage was 59.20% (w/w) in swine hair and the precipitation technique, with relative activities close to 50%. The results are promising for the application of residues and microorganisms in biotechnological processes of economic and environmental interest.

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Section: Enzymatic Precipitationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fusarium oxysporum and Aspergillus sp. as Keratinase Producers Using Swine Hair From Agroindustrial Residues

Preczeski

Dalastra

Czapela

et al. 2020

Front. Bioeng. Biotechnol.

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“…The tests were performed in an ice bath at 4 °C. After centrifugation at 9500 x g, 4 °C and 20 min, the supernatant phase was discarded and the precipitate was resuspended with 50 mM Tris HCl buffer (pH 8.5) (Preczeski et al 2018).…”

Section: Enzymatic Concentrationmentioning

confidence: 99%

Reactional Ultrasonic Systems And Microwave Irradiation For Pretreatment Of Agro-Industrial Waste To Increase Enzymatic Activity

Czapela

Kubeneck

Preczeski

et al. 2020

Preprint

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Pretreatment of keratinous residues using an ultrasonic reaction system provides greater enzymatic production in less time. This is a promising technology for measuring enzyme activity and microwave processes. In the present work, an ultrasonic probe reaction system was used to evaluate the potential of swine hair pretreatment. The pretreated material was submerged with non-pretreated residues for 9 days to obtain the enzyme. Enzyme activity was measured in the extracts obtained using the ultrasonic probe, ultrasonic bath, and microwave. We also used the enzymatic concentration technique with NaCl and acetone. Homemade enzymatic extracts were evaluated for their ability to degrade swine hair and chicken feathers by comparing them with the activities commercial enzymes. Macrobeads gave greater energy dissipation in less time, providing greater enzyme activity (50.8 U/mL over 3 days). In terms of waste degradation, non-pretreated swine hair was more promising. The ultrasonic probe reaction system had the potential to evaluate increased enzyme activity (38.4% relative activity) and the enzyme concentration increased activity by 53.5%. The homemade enzymatic extract showed promise for degradation of keratinous residues.

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“…In general, the conversion increases proportionally with the increase of lipase concentration [14]. Concentration methods such as precipitation [15,16] require costly chemicals such as ammonium sulfate. Another concentration method is membrane separation technology, which has advantages that include energy savings, selectivity, no chemical requirement, and simplicity of operation and scale-up [17].…”

Section: Introductionmentioning

confidence: 99%

Concentration of Lipase from Aspergillus oryzae Expressing Fusarium heterosporum by Nanofiltration to Enhance Transesterification

et al. 2020

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Nanofiltration membrane separation is an energy-saving technology that was used in this study to concentrate extracellular lipase and increase its total activity for biodiesel production. Lipase was produced by recombinant Aspergillus oryzae expressing Fusarium heterosporum lipase (FHL). A sulfonated polyethersulfone nanofiltration membrane, NTR-7410, with a molecular weight cut-off of 3 kDa was used for the separation, because recombinant lipase has a molecular weight of approximately 20 kDa, which differs from commercial lipase at around 30 kDa for CalleraTM Trans L (CalT). After concentration via nanofiltration, recombinant lipase achieved a 96.8% yield of fatty acid methyl ester (FAME) from unrefined palm oil, compared to 50.2% for CalT in 24 h. Meanwhile, the initial lipase activity (32.6 U/mL) of recombinant lipase was similar to that of CalT. The composition of FAME produced from recombinant concentrated lipase, i.e., C14:1, C16:0, C18:0, C18:1 cis, and C18:2 cis were 0.79%, 34.46%, 5.41%, 45.90%, and 12.46%, respectively, after transesterification. This FAME composition, even after being subjected to nanofiltration, was not significantly different from that produced from CalT. This study reveals the applicability of a simple and scalable nanofiltration membrane technology that can enhance enzymatic biodiesel production.

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Efficient and low-cost alternative of lipase concentration aiming at the application in the treatment of waste cooking oils

Cited by 18 publications

References 26 publications

Fusarium oxysporum and Aspergillus sp. as Keratinase Producers Using Swine Hair From Agroindustrial Residues

Fusarium oxysporum and Aspergillus sp. as Keratinase Producers Using Swine Hair From Agroindustrial Residues

Reactional Ultrasonic Systems And Microwave Irradiation For Pretreatment Of Agro-Industrial Waste To Increase Enzymatic Activity

Concentration of Lipase from Aspergillus oryzae Expressing Fusarium heterosporum by Nanofiltration to Enhance Transesterification

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