“…Stress minimization results in the increased viability of cells and process robustness [ 70 , 94 ]. In the so-called Design of Experiments (DoE) setting [ 95 ] the optimization of the expression of recombinant Fab and scFv fragments in stress minimization conditions has been successfully achieved through the selection of media [ 70 , 88 , 96 , 97 ], screening of signal peptide sequences [ 67 , 68 , 75 , 98 ] and optimization of co-expressing chaperone proteins [ 56 , 88 , 99 , 100 , 101 , 102 ]. At the transcriptional level, the concept of “codon harmonization”, a sophisticated version of the codon usage optimization, has largely improved the expression of antibodies fragments [ 60 , 103 ].…”