Efficient Anticancer Effect on Choroidal Melanoma Cells Induced by Tanshinone IIA Photosensitization

Li, Juan; Wei, Diandian; Wang, Jianfeng; Li, Ning; Fan, Yu–Chen; Li, Na; Zhao, Sijie; Li, Kun; Sun, Fenyong

doi:10.1111/php.13399

Cited by 3 publications

(2 citation statements)

References 47 publications

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“…Additionally, it should be non-toxic to normal cells. An example of such a compound is Tanshinone IIA, which accumulates in the nucleus of human choroidal melanoma MUM-2B cells and, upon light activation, generates ROS and induces apoptosis [ 65 ]. Another group of compounds commonly used in PDT is porphyrins and their derivatives.…”

Section: Photodynamic Therapymentioning

confidence: 99%

New Perspectives for Eye-Sparing Treatment Strategies in Primary Uveal Melanoma

Bilmin

Synoradzki

Czarnecka

et al. 2021

Cancers

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Uveal melanoma is the most common intraocular malignancy and arises from melanocytes in the choroid, ciliary body, or iris. The current eye-sparing treatment options include surgical treatment, plaque brachytherapy, proton beam radiotherapy, stereotactic photon radiotherapy, or photodynamic therapy. However, the efficacy of these methods is still unsatisfactory. This article reviews several possible new treatment options and their potential advantages in treating localized uveal melanoma. These methods may be based on the physical destruction of the cancerous cells by applying ultrasounds. Two examples of such an approach are High-Intensity Focused Ultrasound (HIFU)—a promising technology of thermal destruction of solid tumors located deep under the skin and sonodynamic therapy (SDT) that induces reactive oxygen species. Another approach may be based on improving the penetration of anti-cancer agents into UM cells. The most promising technologies from this group are based on enhancing drug delivery by applying electric current. One such approach is called transcorneal iontophoresis and has already been shown to increase the local concentration of several different therapeutics. Another technique, electrically enhanced chemotherapy, may promote drug delivery from the intercellular space to cells. Finally, new advanced nanoparticles are developed to combine diagnostic imaging and therapy (i.e., theranostics). However, these methods are mostly at an early stage of development. More advanced and targeted preclinical studies and clinical trials would be needed to introduce some of these techniques to routine clinical practice.

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Section: Photodynamic Therapymentioning

confidence: 99%

New Perspectives for Eye-Sparing Treatment Strategies in Primary Uveal Melanoma

Bilmin

Synoradzki

Czarnecka

et al. 2021

Cancers

View full text Add to dashboard Cite

show abstract

“…Additionally, it should be non-toxic to normal cells. An example of such a compound is Tanshinone IIA, which accumulates in the nucleus of human choroidal melanoma MUM-2B cells and, upon light activation, generates ROS and induces apoptosis [56]. Another group of compounds commonly used in PDT is porphyrins and their derivatives.…”

Section: Photodynamic Therapymentioning

confidence: 99%

New Perspectives for Eye-Sparing Treatment Strategies in Primary Uveal Melanoma

Bilmin¹,

Synoradzki²,

Czarnecka³

et al. 2021

Preprint

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Uveal melanoma is the most common intraocular malignancy and arises from melanocytes in the choroid, ciliary body, or iris. Radiotherapy is the mainstay of therapy for most patients with localized uveal melanoma. Another RT technique used in the treatment of uveal melanomas is charged-particle radiotherapy. Photodynamic therapy is based on the selective destruction of cancer cells or pathological vessels. High-Intensity Focused Ultrasound (HIFU) is a promising technology of thermal destruction of solid tumors located deep under the skin. The principle of operation is based on the heating of a tumor. Sonodynamic therapy (SDT) induces the reactive oxygen species and kills cancer cells. Electroporation applied in vivo delivers drugs or genetic material from the intercellular space to cells. Iontophoresis is a technique in which using electric current increases the biodistribution of drugs in the eyeball. Transcorneal iontophoresis has been shown to increase the local concentration of antibacterial and antifungal drugs, steroids, DNA, and RNA molecules. Theranosticsincorporates diagnostic imaging and therapy. Although no theranostic markers have been developed specifically for uveal melanoma, some NPs have already found use in ophthalmology. UM presents an unmet clinical need. Novel eye-preserving therapeutic approaches for the localized disease are needed.

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Inhibitory Effect of Tanshinone IIA Extracted from Radix Salviae miltiorrhizae on Melanoma B16F10 Cells

Li,

Chen,

Cheng

et al. 2024

j biobased mat bioenergy

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In this study, we investigate the inhibitory effects of Tanshinone IIA on the proliferation and migration of B16F10 melanoma cells and its potential mechanism of action. We cultured B16F10 melanoma cells in vitro and treated them with varying concentrations of tanshinone IIA. The inhibitory effect of tanshinone IIA on B16F10 cells was assessed using the CCK-8 assay, employing methyl thiazolyl tetrazolium. Melanin content was determined by the NaOH cleavage method, while expression levels of melanin-related proteins were analyzed by Western blotting, and migration-related proteins were quantified using ELISA. When the concentration of Tanshinone IIA reached 20 and 40 μg/mL, there was a decrease in the survival rate of B16F10 melanoma cells. The proliferation inhibition rate of B16F10 melanoma cells gradually increased with the duration of drug action and the concentration of Tanshinone IIA. After 48 hours, there was an increase in melanin content. A comparison of melanin synthesis at the three concentrations yielded an F value of 61.820 and P <0.001. The relative expression levels of TYR, TRP-1, TRP-2, and MITF increased when treated with different concentrations of Tanshinone IIA (P < 0.05). MMP-2 and MMP-9 were downregulated when the concentration of Tanshinone IIA was at 40 and 80 μg/mL respectively (P <0.05). In conclusion, our study demonstrated the inhibitory effects of Tanshinone IIA on growth, proliferation, migration, and melanin synthesis in B16F10 melanoma cells. However, further research is needed to elucidate the specific signal transduction pathway activated by Tanshinone IIA.

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Efficient Anticancer Effect on Choroidal Melanoma Cells Induced by Tanshinone IIA Photosensitization

Cited by 3 publications

References 47 publications

New Perspectives for Eye-Sparing Treatment Strategies in Primary Uveal Melanoma

New Perspectives for Eye-Sparing Treatment Strategies in Primary Uveal Melanoma

New Perspectives for Eye-Sparing Treatment Strategies in Primary Uveal Melanoma

Inhibitory Effect of Tanshinone IIA Extracted from Radix Salviae miltiorrhizae on Melanoma B16F10 Cells

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