Efficient artificial microRNA-mediated resistance against zucchini yellow mosaic virus in zucchini via agroinfiltration

Berbati, Margarita; Kaldis, Athanasios; Voloudakis, Andreas

doi:10.1016/j.jviromet.2023.114805

Cited by 6 publications

(3 citation statements)

References 55 publications

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“…However, small nucleotidic modification via gene editing could render a miRNA permissive to induce ZYMV silencing (inhibition by mRNA cleavage or by mRNA translation) and this approach requires future testing. Furthermore, the identified miRNAs that regulate watermelon’s resistance/susceptibility genes and/or ZYMV RNA could be evaluated via an amiRNA-mediated approach that has been established in watermelon [36].…”

Section: Discussionmentioning

confidence: 99%

“…In order to limit the losses from ZYMV, our lab has employed several approaches to induce resistance against ZYMV infection in cucurbits based on exogenous application of dsRNAs [35] as well as amiRNAs [36]. In the present research, we performed NGS analysis in watermelon to identify the miRNAs that are differentially expressed due to ZYMV infection and to find their potential target host genes.…”

Section: Introductionmentioning

confidence: 99%

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Identification of watermelon genes involved in the ZYMV interaction through a miRNA bioinformatics analysis and characterization ofATRIPandRBOHB

Berbati,

Bousali,

Kaldis

et al. 2023

Preprint

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Small RNA sequencing of healthy and ZYMV-infected watermelon (21 dpi) was performed and bioinformatics analysis identified 353 miRNAs from which 22 known and 331 new miRNAs. Important information about their precursors, their length, the loci of which they originated on watermelon genome are provided. The ZYMV genome could be a target for mir396a-3p, miR8706a, and miR1886i-5p from the miRbase, but none of them was identified in the watermelon miRNAome. Furthermore, watermelon miRNAome does not contain a miRNA targeting ZYMV genome with an expectation score ≤ 3.5. There were 34 resistance genes (CC-NBS-LRR, TIR-NBS-LRR, TIR-NBS) predicted as targets of 32 miRNAs in healthy watermelon. For nine differentially expressed miRNAs the respective target genes (10 in total) were bioinformatically predicted. For cla-new_miR307 (upregulated upon ZYMV infection) and cla-miR166h-3p (downregulated upon ZYMV infection) the targets were predicted to beClaATRIPandClaRBOHB, respectively, withClaATRIPdownregulated andClaRBOHBupregulated upon ZYMV infection. ALSV-mediated VIGS ofClaATRIPrendered watermelon plants more resistant to ZYMV, whereas VIGS ofClaRBOHBresulted in higher levels of ZYMV titer in watermelon. These data suggest thatClaATRIPandClaRBOHBare a susceptibility and resistant gene, respectively. Our results provide new insights in watermelon miRNAome and could propose new strategies for generating resistant watermelon to ZYMV.HighlightsBioinformatics analysis in healthy and ZYMV-infected watermelon plants gave 353 miRNAs, 22 known and 331 new.The watermelon miRNAome identified in the present study does not appear to target the ZYMV genome (sense and reverse complement).The differential expression of ten genes of watermelon in relation to ZYMV infection was validated.The silencing of the two genes,ATRIPandRBOHB, through VIGS strongly suggested thatATRIPis a gene of susceptibility andRBOHBis a gene of resistance.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of watermelon genes involved in the ZYMV interaction through a miRNA bioinformatics analysis and characterization ofATRIPandRBOHB

Berbati,

Bousali,

Kaldis

et al. 2023

Preprint

Self Cite

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“…Overall, the pAMIN14 transgenic line exhibits a greater level of TYLCLV resistance than the pAMIE14 transgenic line, with resistance comparable to that of plants carrying the TY1 resistance gene. Additionally, zucchini resistant to the yellow mosaic virus (ZYMV) can be created via artificial miRNA-mediated methods [145]. These amiRNAs are derived from the gene encoding HC-Pro-a ZYMV protein involved in aphid transmission and cell-to-cell movement thought to be an RNAi repressor [146].…”

Section: Artificial Mirnas In Microbe Resistancementioning

confidence: 99%

microRNAs: Key Regulators in Plant Responses to Abiotic and Biotic Stresses via Endogenous and Cross-Kingdom Mechanisms

Ding,

Li,

et al. 2024

IJMS

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Dramatic shifts in global climate have intensified abiotic and biotic stress faced by plants. Plant microRNAs (miRNAs)—20–24 nucleotide non-coding RNA molecules—form a key regulatory system of plant gene expression; playing crucial roles in plant growth; development; and defense against abiotic and biotic stress. Moreover, they participate in cross-kingdom communication. This communication encompasses interactions with other plants, microorganisms, and insect species, collectively exerting a profound influence on the agronomic traits of crops. This article comprehensively reviews the biosynthesis of plant miRNAs and explores their impact on plant growth, development, and stress resistance through endogenous, non-transboundary mechanisms. Furthermore, this review delves into the cross-kingdom regulatory effects of plant miRNAs on plants, microorganisms, and pests. It proceeds to specifically discuss the design and modification strategies for artificial miRNAs (amiRNAs), as well as the protection and transport of miRNAs by exosome-like nanovesicles (ELNVs), expanding the potential applications of plant miRNAs in crop breeding. Finally, the current limitations associated with harnessing plant miRNAs are addressed, and the utilization of synthetic biology is proposed to facilitate the heterologous expression and large-scale production of miRNAs. This novel approach suggests a plant-based solution to address future biosafety concerns in agriculture.

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Gene silencing by RNA interference: a review

Malakondaiah,

Julius,

Ponnambalam

et al. 2024

GENOME INSTAB. DIS.

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Efficient artificial microRNA-mediated resistance against zucchini yellow mosaic virus in zucchini via agroinfiltration

Cited by 6 publications

References 55 publications

Identification of watermelon genes involved in the ZYMV interaction through a miRNA bioinformatics analysis and characterization ofATRIPandRBOHB

Identification of watermelon genes involved in the ZYMV interaction through a miRNA bioinformatics analysis and characterization ofATRIPandRBOHB

microRNAs: Key Regulators in Plant Responses to Abiotic and Biotic Stresses via Endogenous and Cross-Kingdom Mechanisms

Gene silencing by RNA interference: a review

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