Efficient biodiesel production from rice bran oil using magnetite immobilized-recombinant lipase from probiotic Bacillus licheniformis

Abstract

With increasingly stringent environmental regulations, the pursuit for sustainable energy warrants approaches that are benign-by-design. Lipases have been found to be potential biocatalysts for converting fatty acid esters into biodiesel,...

Probing the Effects of Antimicrobial‐Lysozyme Derivatization on Enzymatic Degradation of Poly(ε‐caprolactone) Film and Fiber

“…Strikingly, new signals at 399.9 and 163.7 eV were observed that could be attributed to the characteristic N 1s and S 2p signals arising from lysozyme and thus ascertained the successful immobilization of the antimicrobial protein. [ 13,50,51 ] It can be noted that the surface chemical changes identified by XPS in both PCL films and ES fibers were identical.…”

“…The codon‐optimized lipase from B. licheniformis was produced in‐house using BL21(DE3)pLysS expression system, as reported in the previous work. [ 50 ] The gram‐negative and gram‐positive bacterial strains E. coli K12 (MTCC 1302) and S. aureus (MTCC 87), respectively, were procured from IMTECH, Chandigarh, India.…”

“…A recombinant lipase from thermophilic Bacillus licheniformis was employed for probing the enzymatic degradability studies owing to its beneficial attributes like probiotic in origin, high purity, high specific activity (≈363 U mg −1 ), and high stability across temperatures ranging from room temperature to 60 °C. [ 50 ] The effect of the surface modification with lysozyme on lipase‐mediated polymer degradation is studied by comparing the efficiency of films versus fibers.…”

Facile Preparation of Bimetallic MOF-derived Supported Tungstophosphoric Acid Composites for Biodiesel Production

“…Then, biodiesel (fatty acid methyl esters, FAME) has been recognized as an appropriate alternative to fossil fuel owing to their renewability, biodegradability, and low emission profiles [3,4]. In general, biodiesel is derived by esterification/transesterification of free fatty acids (FFAs), plant oils, animal fats, waste cooking oils, and microalgae and triglyceride with short-chain alcohols in the presence of homogeneous/heterogeneous catalysts [5]. Although the usage of homogeneous catalysts can achieve a high percentage of conversion rates, it also causes some salient problems such as expensive separation stages and large amounts of chemical wastewater [6].…”

Direct experimental evidence for the boronic acid-mediated bridging of DNA hybrids

“…The field of DNA biotechnology has brought about a revolution in modern biology, with countless applications in various domains, including medicine, forensics, environmental studies, and agriculture. [1][2][3][4][5][6][7][8][9] A widely used approach in this field involves leveraging the chemical reactivity of DNA to produce modified or synthetic nucleotides, which can be used to power an array of cutting-edge technologies such as functional nanostructure assembly, molecular motors, logic gates, CRISPR-Cas9 and biosensors. [10][11][12][13][14][15] On a different note, boronate ester chemistry-arising due to the specific reactivity of boronic acid with 1,2-and 1,3-diols-has garnered considerable interest in the field of chemical biology due to its biocompatibility and distinctive reactivity.…”