Efficient biomass saccharification using a novel cellobiohydrolase from Clostridium clariflavum for utilization in biofuel industry

Abstract

Successful expression of a novel cellobiohydrolase enzyme from Clostridium clariflavum with efficient saccharification potential of plant biomass for the biofuel industry.

Enzymatic hydrolysis of lignocellulosic biomass using a novel, thermotolerant recombinant xylosidase enzyme from Clostridium clariflavum: a potential addition for biofuel industry

“…These results are consistent with previous reports on saccharification of sugarcane bagasse. 43,89,96,97 Effect of recombinant xylosidase on the hydrolysis of wheat straw was observed in similar pattern like higher percentage hydrolysis (8.91%) again pretreated wheat straw biomass. Many reports are present on the plant biomass hydrolysis by xylosidase enzyme from various microorganisms 20,45,84,98–100 but not any report on hydrolysis of recombinant xylosidase enzyme from C. clariflavum yet present.…”

“…1. Other reports [42][43][44][45][46] are present on the cloning and expression of xylosidase gene in E. coli from various sources but till now no report is present on the cloning and expression of xylosidase gene from C. clariavum.…”

“…Similar results were reported in previous reports. 43,[85][86][87][88][89] This pretreatment facilitated the enzymatic hydrolysis of biomass by recombinant xylosidase enzyme. The enzymatic hydrolysis of pre-treated plant biomass is mainly affected by the enzyme loadings, pH of the reaction, temperature of incubation, substrate concentration, reaction time as well as the applied pre-treatment method.…”

Recombinant cellobiohydrolase of Myceliophthora thermophila: characterization and applicability in cellulose saccharification

“…The enzyme activity of rMtCel6A in the presence of metal ions is comparable with the CBH of Clostridium clariflavum and Trichoderma harzianum ; stimulation in enzyme activity was observed in the presence of Cu 2+ and Co 2+ ions (Zafar et al 2021 ; Nawaz et al 2006 ). The activity of CBH of Neocallimastix patriciarum J11 was stimulated by Co 2+ , Mn 2+ and Cu 2+ (Wang et al 2014 ).…”

Progress in Selective Conversion of 5‐Hydroxymethylfurfural to DHMF and DMF

“…[12][13] In order to make full use of biomass resource, fine chemicals, and biological liquid fuel oil can be prepared by hydrogenation, oxidation, hydrolysis, esterification, reductive depolymerisationenzymatic hydrolysis, and catalytic pyrolysis. [14][15][16][17][18][19][20][21][22][23][24][25][26] 5-hydroxymethylfurfural (HMF) is a very momentous platform compound, [27][28][29][30] HMF was obtained though the dehydration of biomass-derived carbohydrates, such as glucose, [31][32][33][34][35] fructose, [36][37][38][39][40] sucrose, [41][42][43][44][45] inulin, [46][47][48][49][50] cellobiose [51][52][53][54][55] and cellulose. [29,[56][57][58]…”