Efficient callus induction from various explants of Jatropha curcas

Abstract

Jatropha curcas L. is a tropical plant of the Euphorbiaceae family, characterized by its high oil content and its ability to grow in a variety of conditions, which made it the most promising non-food biofuel crop in the world. The current study was directed to develop effective methods of tissue culture using this plant. The study was able to induce callus from cotyledons of sterile seedlings, as well as from true leaves taken from the field and from the embryo, using MS medium supplemented with interfering concentrations of Naphthalene acetic acid (NAA) with Benzyl adenine (BA), as the results indicated that The medium containing 0.5 mgL-1 for each of them gave the highest percentage of callus induction for the cotyledonous leaves, true leaves and embryonic peduncle, which reached 100%, 80% and 100%, respectively.