Efficient cell chatting between embryo and uterus ensures embryo implantation

Deng, Wenbo; Wang, Haibin

doi:10.1093/biolre/ioac135

Cited by 7 publications

(4 citation statements)

References 108 publications

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“…The P4-and E2-induced signaling pathways identified in mouse studies, with some examples from rat studies, are briefly reviewed. The details of the underlying mechanisms have been extensively reviewed elsewhere [31][32][33][34].…”

Section: Basic Molecular Pathways Established From Mouse Modelsmentioning

confidence: 99%

Molecular approaches to mammalian uterine receptivity for conceptus implantation

KUBOTA

2024

J. Reprod. Dev.

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Mammalian reproduction is more inefficient than expected and embryo/conceptus implantation into the maternal endometrium is considered to be a rate-limiting process. Although extensive physiological and structural diversity exists among mammalian species, the basic molecular mechanisms underlying successful implantation are conserved. The extensive use of genetically engineered mouse models has provided considerable information on uterine receptivity for embryo implantation. The molecular mechanisms and cellular processes identified thus far require further validation in other mammalian species. In this review, representative ovarian steroid hormone-induced signaling pathways controlling uterine adaptation are presented based on the results of rodent studies.Selected examples of functional conservation in mammals, such as humans and cattle, are briefly described. To date, molecular therapeutic trials for fertility improvement have not been conducted.Considerable efforts are required to provide further understanding of these molecular mechanisms. Such understanding will contribute to the development of reliable clinical diagnostics and therapeutics for implantation failure, leading to reproductive success in a wide variety of mammals in the future.

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Section: Basic Molecular Pathways Established From Mouse Modelsmentioning

confidence: 99%

Molecular approaches to mammalian uterine receptivity for conceptus implantation

KUBOTA

2024

J. Reprod. Dev.

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“…Endometrial receptivity is limited to 4–5 days during blastocyst implantation. The implantation period of the endometrium is called the window of implantation (WOI), during which the secretion of various cytokines in response to ovarian steroid hormones is regulated [ 1 , 2 ].…”

Section: Introductionmentioning

confidence: 99%

A case of discrepancy between three ERA tests in a woman with repeated implantation failure complicated by chronic endometritis

Ota

Takahashi

Mitsui³

et al. 2022

BMC Pregnancy Childbirth

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Background Endometrial receptivity array (ERA) is used to determine the timing of embryo transfer (ET) synchronized with the window of implantation (WOI). The effectiveness and evaluation of ERAs in women with recurrent implantation failure remain controversial. We report the case of a patient with recurrent implantation failure that raises the issue of reproducibility of ERA tests. Case report A 36-year-old Japanese woman with secondary infertility who had previously given birth failed to conceive after three frozen-thawed embryo transfer (FET) cycles. An ERA test was conducted to confirm the WOI. The first ERA test was performed 125 h after progesterone exposure. The laboratory reported that the endometrium was in a non-receptive (post-receptive) phase, and recommended retesting 101 h after progesterone exposure. A simultaneous chronic endometritis (CE) test showed a score of 3. After the antibiotics administration to treat CE, the second ERA test was performed after 101 h of progesterone exposure. The laboratory reported that the endometrium had not reached the WOI and estimated the WOI to be 113 ± 3 h after progesterone exposure. The third ERA test was performed 113 h after progesterone exposure. The laboratory reported that the endometrium was in a non-receptive (pre-receptive) phase and estimated the WOI to be 137 ± 3 h after progesterone exposure. A CE test performed at the same time as the second and third ERA tests showed a score of 1 for the collected endometrium. According to the third ERA test results, the vitrified-warmed blastocyst was transferred at 137 h of progesterone exposure. Pregnancy was achieved and the patient had an uncomplicated vaginal delivery at 39 weeks. One year later, another pregnancy was achieved after FET at 137 h of progesterone exposure, and the patient delivered at 33 weeks due to an unexpected membrane rupture. Conclusion Because the results of the ERA test may vary in the presence of CE, CE should be diagnosed simultaneously with or before conducting ERA tests. If CE is diagnosed, ERA testing should be performed after treatment with antimicrobials or other drugs.

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Embryo implantation is a crucial early event for successful pregnancy and is regulated by diverse genes and proteins, as well as by steroid hormones, oestrogen, progesterone and other hormones (Deng & Wang, 2022). Sheep blastocysts extend from an initial ball approximately 10 mm in length to a 250 mm filamentous structure.This rapid extension of the trophoblast that forms the outer layer of the blastocyst is essential for creating a large surface area for nutrient absorption and exchange of gases (Ali & Derar, 2021) and is

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mentioning

confidence: 99%

“…Embryo implantation is a crucial early event for successful pregnancy and is regulated by diverse genes and proteins, as well as by steroid hormones, oestrogen, progesterone and other hormones (Deng & Wang, 2022). Sheep blastocysts extend from an initial ball approximately 10 mm in length to a 250 mm filamentous structure.…”

mentioning

confidence: 99%

PPARγ regulates lipid metabolism and viability of sheep trophoblast cells

Hao,

Liu,

Chen

et al. 2023

Reprod Domestic Animals

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Peroxisome proliferator‐activated receptor γ (PPARγ) is highly expressed in trophoblast tissues in pregnancy during which the protein participates in diverse events, including embryo implantation and placental formation. However, little is known about the role of PPARγ in embryonic development. This study investigated the function of PPARγ in sheep trophoblast cells. The coding sequence of sheep PPARγ encoded 475 amino acids and included one synonymou mutation compared with the sheep reference sequence for PPARγ. The PPARγ protein was localized in the nucleus and cytoplasm of sheep trophoblasts. The relative expression of PPARγ was elevated in cells treated with rosiglitazone and reduced following administration of GW9662. Activation of PPARγ promoted cell proliferation and mobility, but inhibited apoptosis. In addition, stimulation of PPARγ promoted the expression of lipid metabolism‐related genes FABP4 and PLIN2. The expression of prostaglandin metabolism‐related genes PLA2G4A, PTGS2 and PTGES also was upregulated significantly in trophoblast cells when PPARγ was activated. In contrast, activation of PPARγ did not impact expression of the prostaglandin‐related genes PGFS and SLCO2A1. At the same time, activation of PPARγ activity increased the ratio of PGE2 to PGF2α. Furthermore, fluorescence labelling showed that the numbers of cell lipid droplets increased after stimulation of PPARγ activity, but decreased when PPARγ was inhibited. In conclusion, PPARγ is critical for the regulation of lipid metabolism and prostaglandin synthesis and secretion in sheep trophoblast cells and also has a potent effect on cell proliferation and viability.

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Efficient cell chatting between embryo and uterus ensures embryo implantation

Cited by 7 publications

References 108 publications

Molecular approaches to mammalian uterine receptivity for conceptus implantation

Molecular approaches to mammalian uterine receptivity for conceptus implantation

A case of discrepancy between three ERA tests in a woman with repeated implantation failure complicated by chronic endometritis

PPARγ regulates lipid metabolism and viability of sheep trophoblast cells

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