Efficient combination of ionic-liquid-based ultrasound-assisted extraction, complex chromatography, and molecular docking for screening of acetylcholinesterase inhibitors from Ganoderma atrum

Hou, Wanchao; Liu, Chunming; Li, Sainan; Niu, Huazhou; Liu, Zhen; Zhang, Yuchi; Jin, Young-Woo; Li, Xuwen

doi:10.1007/s11694-022-01699-2

Cited by 3 publications

(1 citation statement)

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“…It exhibited antitumor activities against human non-small cell lung cancer (NCI-H 460) cell line, anticancer against human breast cancer (MCF-7), human neuroblastoma (SH-SY5Y) cell lines, colorectal adenocarcinoma (Caco-2) cell line, breast, mammary gland cancer (MDA-MB-231) cell line and antimicrobial activity against a fungus Cryptococcus neoformans, respectively. In addition, this compound was isolated from Ganoderma atum extract [61] exhibited the potential prevention and treatment of neurodegenerative diseases. 16).…”

Section: Extraction Of the Phellinus Sp Mushroommentioning

confidence: 99%

Antiproliferative agents from mushroom phellinus sp.

Pichayaphinyo

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In this study,�Phellinus�powdered (600 g) was extracted with methanol and DI water. The methanolic crude extract of the mushroom was partitioned with hexane, dichloromethane, ethyl acetate and methanol respectively. All crude extracts were evaluated antiproliferative activity against lung cancer (A549) and neuroblastoma (SH-SY5Y) cell lines by MTT assay. It was found that the dichloromethane extract (CDE) exhibited the strongest antiproliferative activity with half maximal inhibitory concentration (IC50) values of 132.4 and 106.87 ?g/mL against human lung cancer and neuroblastoma cell lines, respectively. The ethyl acetate extract (CEE) exhibited moderate antiproliferative activity with IC50�values of 138.64 and >250 ?g/mL against SH-SY5Y and A549 cell lines, respectively. The hexane extract, methanol�extract and aqueous extracts from reflux extraction and decoction exhibited weak antiproliferative activity against both cancer cell lines with IC50�>250 ?g/mL. Isolation by repeated column chromatographic, followed by NMR characterization, ergosta-7,22-dien-3-one and 7-methoxyindole-3-carboxylic acid methyl ester, 7-methoxyindole-3-carboxaldehyde and protocatechualdehyde from hexane extract, dichloromethane extract and ethyl acetate extract, respectively.

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Section: Extraction Of the Phellinus Sp Mushroommentioning

confidence: 99%