Efficient CRISPR/Cas9‐mediated genome editing in sheepgrass (<i>Leymus chinensis</i>)

Lin, Zhelong; Chen, Lei; Tang, Shanjie; Zhao, Mengjie; Li, Tong; You, Jia; You, Changqing; Li, Boshu; Zhao, Qinghua; Zhang, Dongmei; Wang, Jianli; Shen, Zhongbao; Song, Xianwei; Zhang, Shuaibin; Cao, Xiaofeng

doi:10.1111/jipb.13567

Cited by 5 publications

(2 citation statements)

References 31 publications

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“…Sheepgrass transformation by Agrobacterium was modified from the described method ( 36 , 92 ). In short, mature grains of sheepgrass were sterilized with 75% ethanol for 5 min and 20% sodium hypochlorite solution for 15 min and rinsed five times with sterile water Then, the sterilized mature grains were placed on MS medium containing 2 mg L –1 2,4-dichlorophenoxyacetic acid for callus induction at 28 °C in the dark.…”

Section: Methodsmentioning

confidence: 99%

Genome evolution and initial breeding of the Triticeae grass Leymus chinensis dominating the Eurasian Steppe

Li,

Tang,

et al. 2023

Proc. Natl. Acad. Sci. U.S.A.

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Leymus chinensis , a dominant perennial grass in the Eurasian Steppe, is well known for its remarkable adaptability and forage quality. Hardly any breeding has been done on the grass, limiting its potential in ecological restoration and forage productivity. To enable genetic improvement of the untapped, important species, we obtained a 7.85-Gb high-quality genome of L. chinensis with a particularly long contig N50 (318.49 Mb). Its allotetraploid genome is estimated to originate 5.29 million years ago (MYA) from a cross between the Ns-subgenome relating to Psathyrostachys and the unknown Xm-subgenome. Multiple bursts of transposons during 0.433–1.842 MYA after genome allopolyploidization, which involved predominantly the Tekay and Angela of LTR retrotransposons, contributed to its genome expansion and complexity. With the genome resource available, we successfully developed a genetic transformation system as well as the gene-editing pipeline in L. chinensis . We knocked out the monocot-specific miR528 using CRISPR/Cas9, resulting in the improvement of yield-related traits with increases in the tiller number and growth rate. Our research provides valuable genomic resources for Triticeae evolutionary studies and presents a conceptual framework illustrating the utilization of genomic information and genome editing to accelerate the improvement of wild L. chinensis with features such as polyploidization and self-incompatibility.

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Section: Methodsmentioning

confidence: 99%

Genome evolution and initial breeding of the Triticeae grass Leymus chinensis dominating the Eurasian Steppe

Li,

Tang,

et al. 2023

Proc. Natl. Acad. Sci. U.S.A.

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“…), an efficient Agrobacterium-mediated CRISPR/Cas9 genome editing platform has been developed. By targeting the Teosinte Branched1 (TB1) gene, an increase in tiller number and biomass was observed, demonstrating the method's effectiveness for gene function studies and breeding in sheepgrass [52]. The study also investigated genome evolution and initial breeding in sheepgrass, suggesting that genome editing could play a pivotal role in enhancing the yield-related traits.…”

Section: Genome Editingmentioning

confidence: 98%

Advances in Molecular Breeding of Forage Crops: Technologies, Applications and Prospects

Chen

2024

Agriculture

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Molecular breeding has revolutionized the improvement of forage crops by offering precise tools to enhance the yield, quality, and environmental resilience. This review provides a comprehensive overview of the current technologies, applications, and future directions in the field of forage crop molecular breeding. Technological advancements in the field, including Quantitative Trait Loci (QTL) mapping, Genome-Wide Association Studies (GWASs), genomic selection (GS), and genome-editing tools such as CRISPR-Cas9, have significantly advanced the identification and incorporation of beneficial traits into forage species. These approaches have dramatically shortened the breeding cycles and increased the efficiency of developing cultivars with improved yield, disease resistance, stress tolerance, and nutritional profiles. The implementation of these technologies has led to notable successes, as demonstrated by case studies on various forage crops, showcasing enhanced forage quality and adaptability to challenging environmental conditions. Furthermore, the integration of high-throughput phenotyping with advanced bioinformatics tools has streamlined the management of large-scale genomic data, facilitating more precise selection and breeding decisions. Looking ahead, this review explores the potential of emerging technologies, such as the application of artificial intelligence in predictive breeding, along with the associated ethical and regulatory considerations. While we stand to gain benefit from these innovations, the future of molecular breeding in forage crops must also confront the challenges posed by climate change and the imperative of sustainable agricultural practices. This review concludes by emphasizing the transformative impact of molecular breeding on the improvement of forage crop and the critical need for ongoing research and collaboration to fully realize its potential.

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