2015
DOI: 10.1038/srep12217
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Efficient CRISPR/Cas9-mediated Targeted Mutagenesis in Populus in the First Generation

Abstract: Recently, RNA-guided genome editing using the type II clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein (Cas) system has been applied to edit the plant genome in several herbaceous plant species. However, it remains unknown whether this system can be used for genome editing in woody plants. In this study, we describe the genome editing and targeted gene mutation in a woody species, Populus tomentosa Carr. via the CRISPR/Cas9 system. Four guide RNAs (gRNAs) were designed to t… Show more

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Cited by 371 publications
(285 citation statements)
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“…When RNA-guided genome editing and targeted gene mutation via the CRISPR/Cas9 system was used to edit the genome of Populus tomentosa, four gRNAs were designed to target distinct poplar genomic sites of the phytoene desaturase gene 8 (PtoPDS) that are followed by the protospacer-adjacent motif (Fan et al 2015;Tsai and Xue 2015). After Agrobacterium-mediated transformation, an obvious albino phenotype was observed in transgenic poplar plants.…”
Section: Populusmentioning
confidence: 99%
“…When RNA-guided genome editing and targeted gene mutation via the CRISPR/Cas9 system was used to edit the genome of Populus tomentosa, four gRNAs were designed to target distinct poplar genomic sites of the phytoene desaturase gene 8 (PtoPDS) that are followed by the protospacer-adjacent motif (Fan et al 2015;Tsai and Xue 2015). After Agrobacterium-mediated transformation, an obvious albino phenotype was observed in transgenic poplar plants.…”
Section: Populusmentioning
confidence: 99%
“…Para superar las dificultades propias de las especies perennes, se han propuesto t茅cnicas modernas como la edici贸n de genes con CRISPR/Cas9 (Fan et al, 2015), as铆 como el uso de vectores virales con los cuales se hacen transformaciones transitorias que soslayan el cultivo de tejidos (Gleba et al, 2004;Yamagishi et al, 2011).…”
Section: El Uso De La Estrategia De Inducci贸n De Floraci贸n En Ciruelaunclassified
“…CRISPR/Cas9 has been widely adopted for genome editing in plants. Over 25 plant species and over 100 genes have been subject to successful editing with CRISPR/Cas9, and many desirable traits in major crops have been created [13,[34][35][36]. However, a crucial current concern for the CRISPR/Cas9 system is the potential for higher off-target effects than with TALENs [21,29,37], which must be addressed for widespread use in crop breeding and gene therapy [38,39].…”
Section: Risks Directly Associated With Off-target Edits To Crop Genomesmentioning
confidence: 99%