The brain stands out as the most intricate organ in the human body, governing cognitive, behavioral, and emotional functions. It is susceptible to various diseases, ranging from injuries to cancers and neurodegenerative conditions, making brain disorders a significant cause of disability and mortality. Overcoming challenges such as delivery, specificity, and toxicity has been a persistent issue in developing drugs that enhance brain structure and function, especially those that can traverse the complex barriers surrounding the brain. Nanotechnology represents a groundbreaking approach to address the unique challenges associated with Autism Spectrum Disorder (ASD). One significant challenge in ASD is early and accurate diagnosis. Nanotechnology can contribute to the development of highly sensitive and specific diagnostic tools, enabling the identification of biomarkers associated with ASD at an earlier stage. These nanoscale devices may facilitate a more precise understanding of the underlying biological mechanisms, leading to improved diagnostic capabilities. In terms of intervention, nanotechnology can enhance drug delivery systems, allowing for the more targeted and efficient administration of medications. This targeted drug delivery can potentially mitigate side effects while maximizing the therapeutic impact, addressing some of the challenges in managing the diverse symptoms of ASD. Despite the promising potential of nanotechnology in addressing ASD challenges, it is essential to approach these innovations with ethical considerations, ensuring that the benefits are accessible and equitable for individuals with ASD. Ongoing research and collaboration between experts in nanotechnology and autism can lead to transformative advancements in understanding, diagnosing, and managing ASD. This review delves into the application of nanotechnology in diagnosing and treating ASD and shedding light on the promising role of nanoparticles.