Efficient derivation of osteoprogenitor cells from induced pluripotent stem cells for bone regeneration

Dogaki, Yoshihiro; Lee, Sang Yang; Niikura, Takahiro; Igarashi, Takashi; Okumachi, Etsuko; Waki, Takahiro; Kakutani, Kenichiro; Nishida, Kotaro; Kuroda, Ryosuke; Kurosaka, Masahiro

doi:10.1007/s00264-014-2440-9

Cited by 14 publications

(20 citation statements)

References 34 publications

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“…Previous studies have demonstrated the osteogenic potential of iPSCs (4,29). Although successful bone healing/ regeneration occurred when using these cells, teratoma formation was observed in 20% of the mice with transplanted osteogenically-induced iPSCs (30).…”

Section: Discussionmentioning

confidence: 97%

Notch signaling partly regulates the osteogenic differentiation of retinoic acid-treated murine induced pluripotent stem cells

Osathanon

Manokawinchoke

Egusa

et al. 2017

Journal of Oral Science

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Notch signaling is involved in osteogenic differentiation; however, its role differs depending on cell type and differentiation stage. Here, we investigated the involvement of Notch signaling in the osteogenic differentiation of retinoic acid-treated embryoid bodies derived from mouse gingival fibroblast-derived induced pluripotent stem cells (mGF-iPSCs). When cultured in osteogenic media, mGF-iPSCs showed an increase in their expression of osteogenic marker genes and deposited a mineralized matrix. Furthermore, increased levels of mRNA for Notch1, Notch2, and Hey1 were observed. In the presence of DAPT, a Notch signaling inhibitor, during osteogenic induction, mRNA levels for osteogenic marker genes were significantly decreased; however, no difference was noted in mineral deposition. Moreover, activation of Notch signaling using Jagged1-immobilized surfaces resulted in a slight increase of in vitro mineralization on days 3 and 7 of osteogenic induction. Significant upregulation of Dlx5, Bsp, and Col I mRNA expression was observed in mGF-iPSCs cultured on Jagged1 surfaces. In conclusion, inhibition and activation of Notch signaling was shown to decrease and increase mGF-iPSC osteogenic differentiation, respectively. However, the responses were not robust, suggesting the involvement of additional signaling pathways.

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Section: Discussionmentioning

confidence: 97%

Notch signaling partly regulates the osteogenic differentiation of retinoic acid-treated murine induced pluripotent stem cells

Osathanon

Manokawinchoke

Egusa

et al. 2017

Journal of Oral Science

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“…A previous study showed that rat BMMSCs showed increased proliferation and differentiation into a neurogenic lineage when grown on gelatin-coated plates as compared to noncoated plates, whereas adipose and osteogenic differentiation potentials were unaffected by gelatin [10]. In other studies using human or mouse derived iMSCs, osteogenic or chondrogenic differentiation potential was induced by gelatin or collagen coating, while they did not directly compare iMSC differentiation potential by deriving on the two types of substrates [3, 12, 15, 26]. The present study investigated for the first time how gelation or collagen coating affects the differentiation potential of derived iMSCs in culture.…”

Section: Discussionmentioning

confidence: 99%

“…Several reports have shown that iMSC characteristics such as differentiation potentials in vitro and in vivo are similar to those of primary progenitor cells [11], but it is not known whether extracellular components such as gelatin and collagen significantly affect iMSC characteristics in its derivation process. Although several studies have successfully reported the derivation of human iMSCs from iPSCs [3, 12–14], a simple and efficient method for inducing mouse iMSCs has not yet been established [15, 16]. However, the safety of further clinical applications must be evaluated in appropriate animal models including genetically modified mouse, which requires the establishment of simple and effective derivation method for mouse iMSCs in addition to human cells.…”

Section: Introductionmentioning

confidence: 99%

Differentiation and Molecular Properties of Mesenchymal Stem Cells Derived from Murine Induced Pluripotent Stem Cells Derived on Gelatin or Collagen

Obara¹,

Takizawa²,

Tomiyama³

et al. 2016

Stem Cells International

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The generation of induced-pluripotential stem cells- (iPSCs-) derived mesenchymal stem cells (iMSCs) is an attractive and promising approach for preparing large, uniform batches of applicable MSCs that can serve as an alternative cell source of primary MSCs. Appropriate culture surfaces may influence their growth and differentiation potentials during iMSC derivation. The present study compared molecular properties and differentiation potential of derived mouse iPS-MSCs by deriving on gelatin or collagen-coated surfaces. The cells were derived by a one-step method and expressed CD73 and CD90, but CD105 was downregulated in iMSCs cultured only on gelatin-coated plates with increasing numbers of passages. A pairwise scatter analysis revealed similar expression of MSC-specific genes in iMSCs derived on gelatin and on collagen surfaces as well as in primary mouse bone marrow MSCs. Deriving iMSCs on gelatin and collagen dictated their osteogenic and adipose differentiation potentials, respectively. Derived iMSCs on gelatin upregulated Bmp2 and Lif prior to induction of osteogenic or adipose differentiation, while PPARγ was upregulated by deriving on collagen. Our results suggest that extracellular matrix components such as gelatin biases generated iMSC differentiation potential towards adipose or bone tissue in their derivation process via up- or downregulation of these master genes.

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“…Compared to Other Types of Cells. The osteogenic differentiation ability of iPSCs-MSCs in comparison to MSCs was examined in a variety of studies [86,[93][94][95]. A study on iPSCs showed a delayed expression of osteogenic markers such as COL1A1 and bone sialoprotein (BSP) as well as weaker osteoblastic differentiation and mineral deposition, compared to human BM-MSCs in vitro [57].…”

Section: Osteogenic Differentiation Capability Of Ipscsmentioning

confidence: 99%

Induced Pluripotent Stem Cells in Dental and Nondental Tissue Regeneration: A Review of an Unexploited Potential

Radwan

Rady

Abbass

et al. 2020

Stem Cells International

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Cell-based therapies currently represent the state of art for tissue regenerative treatment approaches for various diseases and disorders. Induced pluripotent stem cells (iPSCs), reprogrammed from adult somatic cells, using vectors carrying definite transcription factors, have manifested a breakthrough in regenerative medicine, relying on their pluripotent nature and ease of generation in large amounts from various dental and nondental tissues. In addition to their potential applications in regenerative medicine and dentistry, iPSCs can also be used in disease modeling and drug testing for personalized medicine. The current review discusses various techniques for the production of iPSC-derived osteogenic and odontogenic progenitors, the therapeutic applications of iPSCs, and their regenerative potential in vivo and in vitro. Through the present review, we aim to explore the potential applications of iPSCs in dental and nondental tissue regeneration and to highlight different protocols used for the generation of different tissues and cell lines from iPSCs.

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Efficient derivation of osteoprogenitor cells from induced pluripotent stem cells for bone regeneration

Abstract: These findings indicate that our novel direct-plating method provides a clinically applicable, simple, and labour-efficient system for generating large numbers of homogeneous iPSC-derived osteoprogenitor cells for bone regeneration.

Cited by 14 publications

References 34 publications

Notch signaling partly regulates the osteogenic differentiation of retinoic acid-treated murine induced pluripotent stem cells

Notch signaling partly regulates the osteogenic differentiation of retinoic acid-treated murine induced pluripotent stem cells

Differentiation and Molecular Properties of Mesenchymal Stem Cells Derived from Murine Induced Pluripotent Stem Cells Derived on Gelatin or Collagen

Induced Pluripotent Stem Cells in Dental and Nondental Tissue Regeneration: A Review of an Unexploited Potential

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