Efficient detection of cholesterol using ZnO thin film based matrix

Abstract

Zinc oxide (ZnO) thin films grown by pulsed laser deposition on indium-tin-oxide (ITO)-coated corning glass are used to immobilise cholesterol oxidase (ChOx) and for cholesterol detection. Cyclic voltammetric studies indicate that the ChOx/ZnO/ITO/glass bioelectrode is sensitive to the detection of cholesterol in 25-500 mg/dL range. A relatively low value of enzyme's kinetic parameter (Michaelis-Menten constant; 1.92 mM) indicates enhanced enzyme affinity of ChOx to cholesterol. The prepared bioelectrode is found to be exhibiting high shelf life of 12 weeks having negligible interference with the presence of other biomolecules present in human serum indicating promising application of ZnO thin films for cholesterol sensing.

Deposition of nanomaterials: A crucial step in biosensor fabrication

“…Moreover, this method is not widely used to spray a variety of nanomaterials because of problems related to ink stability. [292][293][294][295][296]. Other nanomaterials have also been used to make thin films for biosensing applications [297][298][299][300][301][302][303].…”

“…Most importantly, to grow different ZnO nanostructures on substrates/electrodes, a thin film of ZnO seed layer (thin film) is necessary. Due to the excellent properties of the ZnO, deposited thin films of the ZnO were also used to fabricate biosensors[292][293][294][295][296]. Recently, ZnO films formed using ALD have provided a good platform to fabricate biosensors for Grapevine virus A-type proteins, cholesterol, DNA, uric acid, urea, and glucose detection…”

ZnO nanoparticles and β-cyclodextrin containing molecularly imprinted polymers for gravimetric sensing of very-low-density lipoprotein

“…Zinc oxide (ZnO) is a wide bandgap n-type semiconductor of the hexagonal structure having unique piezoelectric and optical properties. ZnO is biocompatible due to its high isoelectric point of 9.5 [23] and has some attractive characteristics such as high specific surface area, chemical stability, nontoxicity, and strong adsorption ability. These characteristics make ZnO a prominent material to be used in biosensors [23][24][25].…”

Development of a paper‐type tyrosinase biosensor for detection of phenolic compounds

“…), that is, hydroxylation of monophenols to o ‐diphenols (monophenolase activity) and oxidation of o ‐diphenols to o ‐quinones (diphenolase activity) . Different support materials and immobilization techniques have previously been used for development of tyrosinase‐based biosensors . In the present work, a tyrosinase‐based paper biosensor is developed for determination of phenolic compounds, using a paper‐strip absorption method.…”