Efficient differentiation of human pluripotent stem cells into cardiomyocytes on cell sorting thermoresponsive surface

Cardiac ultrastructure inspired matrix induces advanced metabolic and functional maturation of differentiated human cardiomyocytes

“…Adult human cardiomyocytes expressed higher levels of transcripts encoding integrins specific for widely used matrix peptide motifs, including RGD and collagen-binding a11, a10, and a1 subunits. a7 and a3, receptors for laminin, were also highly expressed in adult heart, although a6 expression was low (Burridge et al, 2014;Sung et al, 2020). In addition, a8 was also upregulated with high specificity for nephronectin, responsible for cardiac development and cardiomyocyte adhesion (Patra et al, 2011(Patra et al, , 2012 (Figure 1A).…”

Effect of extracellular matrix proteins on the differentiation of human pluripotent stem cells into mesenchymal stem cells

“…Journal of Materials Chemistry B Culture and hMSC differentiation of hPSCs on ECM-coated dishes hESCs (H9) and hiPSCs (HM-5) were maintained on rVN (5 mg mL À1 )-coated dishes in Essential 8 (E8, xeno-free) medium. [27][28][29] hPSCs were differentiated into hMSCs according to the protocol published by Li et al 23 with some modifications.…”

Multitunable Thermoresponsive and Aggregation Behaviors of Linear and Cyclic Polyacrylamide Copolymers Comprising Heterofunctional Y Junctions

“…The emergence of stimuli-responsive polymers undergoing stimuli-triggered dramatic variations of physical properties has revolutionized artificial smart materials. − Among them, thermoresponsive polymers sensitive to the environmental temperature have attracted much attention due to the potential applications in distinct fields such as drug delivery for cancer therapy, − nanocatalysis, , and surface modification. − They can exhibit lower critical solution temperature (LCST) and/or upper critical solution temperature (UCST) behaviors in suitable media. Compared with well-documented LCST polymers, UCST polymers in aqueous systems have been much less reported due to extreme environmental sensitivity. − For UCST polymers, supramolecular interactions such as hydrogen bonding (HB) and electrostatic interactions (EIs) are gradually weakened upon heating, leading to improved solubility.…”