Efficient Direct Organogenesis from Shoot Tips and Nodal Segments of Ash Gourd (Benincasa Hispida L.)

Abstract

Abstract:The effect of external application of phytohormone on explants viz., shoot tips and nodal segments of ash gourd (Benincasa hispida L.) was tested on the frequency of shoot and root induction. Shoot tips and nodal segments were cultured on MS medium supplemented with different concentrations and combinations of cytokinins (BAP, Kinetin), auxin (IBA, NAA) and gibberellic acid (GA 3 ) for multiple shoot formation and root induction. The highest number (up to 90%) of multiple shoot formation was obtained from the shoot tips in MS medium fortified with 1.5 mg/l BAP + 0.2 mg/l GA 3, where average number of shoots per culture was 5.55. In case of nodal segment, better response (up to 78%) for shoot multiplication was found in MS with 2.0 mg/l BAP + 0.2 mg/l GA 3. The concentration of 1.0 mg/l IBA was found to be most effective for root initiation in microshoot developed from both types of explants. Rooted plantlets were acclimatatized and established in sandy soil with good survival rate.Key words: Organogenesis, ash gourd, MS medium, shoot tips, nodal segments mvivskt PvjKz govi KvÊkxl© I ce© vsk G·c− v›U e¨envi K ‡i KvÛgy Kz j I gj Drcv` ‡b dvB ‡Uvni ‡gv ‡bi cÖ ‡qv ‡Mi e¨vwn¨K cÖ fve cix ¶v Kiv n ‡q ‡Q| eûgy Kz j Ges gj Rb¥vevi D ‡Ï ‡k¨ KvÊkxl© Ges ce© vsk¸ ‡jv GgGm wgwWqv ‡gi mv ‡_ wewfboe Nb ‡Z¡ i I wgkÖ ‡Yi mvB ‡UvKvBwbÝ (weGwc I KvB ‡bwUb), Aw·b (AvBweG I GbGG) Ges wR ‡eª wjK GwmW (wRG 3 ) mn ‡hv ‡M KvjPvi Kiv n ‡q ‡Q| 1.5 wgwjMÖ vg/wjUvi weGwc + 0.2 wgwjMÖ vg /wjUvi wRG 3 ni ‡gvb¸wj GgGm wgwWqv ‡gi g ‡a¨ e¨envi K ‡i KvÊkxl© †_ ‡K me ‡P ‡q †ewk (90% ch© šÍ ) eû-KvÛgy Kz j Drcv`b m¤¢ e n ‡q ‡Q Ges †hLv ‡b KvjPvi cÖ wZ gy Kz j msL¨v `u vwo ‡q ‡Q 5.55| ce© vs ‡ki † ¶ ‡Î KvÛgy Kz j Drcv` ‡bi Rb¨ 2.0 wgwjMÖ vg/wjUvi weGwc + 0.2 wgwjMÖ vg/wjUvi wRG 3 e¨envi K ‡i fvj dj cvIqv †M ‡Q (78% ch© šÍ )| Dfq ai ‡bi G·c− v ‡›Ui † ¶ ‡Î 1.0 wgwjMÖ vg/ wjUvi Nb ‡Z¡ i AvBweG e¨envi K ‡i ¶zª KvÛgy Kz j †_ ‡K me© vwaK gj Drcv`b cwijw ¶Z n ‡q ‡Q| wkKo MRv ‡bv AYy Pviv¸ ‡jv ‡K evjy hy ³ gvwU ‡Z Lvc LvIqv ‡bv Ges ¯' vqxKiY Kiv n ‡q ‡Q| G ‡`i †eu ‡P _vKvi nvi h ‡_ó fv ‡jv|

Plant Regeneration from Cotyledon Explants of Bittergourd as influenced by Adenine Sulphate and D-Biotin

“…After three weeks of culture, root formation was noted in the cultured shoots (Figure 5). This is supported by Kausar et al (2013) and…”