Efficient DNA sequencing with a pulsed semiconductor laser and a new fluorescent dye set

Fluorescent Oligonucleotides - Versatile Tools as Probes and Primers for DNA and RNA Analysis

“…Today the development of new dyes is obviously heading towards the infrared region allowing the employment of less expensive photodiode detector systems and improving the signal-to-noise ratio. 27,28 Additionally, new energy transfer dyes consisting of both a fluorescein and a rhodamine derivative show improved performance for low concentration detection of nucleic acids, e.g. for DNA sequencing ( Figure 3).…”

A fully automated multicapillary electrophoresis device for DNA analysis

“…For DNA analysis, a number of covalently bound dyes (e.g., [26,30,32,34, 37±41]) and intercalating dyes (e.g., [42±45]) are available to date, covering a large range of the spectrum. A ªclassicalº combination is the argon ion laser together with fluorescein derivatives as labels.…”

Counting single molecules in living cells at high resolution by spectrally resolved fluorescence lifetime imaging microscopy (SFLIM) and coincidence analysis

“…Fortunately, fluorescence photons carry several pieces of information that can be accessed and that can help to transmit the changes of the molecules investigated to the experimenter. Besides the fact that fluorescence intensity, lifetime [22,[23][24][25][26][27][28][29], emission spectrum [30,31], and anisotropy [32,33] can be used to monitor dynamics of an investigated system one should recall that the number of photons detected from a single fluorescent molecule is limited. An elegant way to further improve the information obtainable from a single fluorescent molecule relies on the use of these four different fluorescence characteristics, if possible in parallel [34,35] Figure 1: Schematic representation of the extended SFLIM setup.…”