Efficient enzymatic hydrolysis and simultaneous saccharification and fermentation of sugarcane bagasse pulp for ethanol production by cellulase from Penicillium oxalicum EU2106 and thermotolerant Saccharomyces cerevisiae ZM1-5

Isolation and characterization of a novel Penicillium oxalicum strain Z1-3 with enhanced cellobiohydrolase production using cellulase-hydrolyzed sugarcane bagasse as carbon source

“…The fungal strains used throughout consisted of the isolates described in this study and P. oxalicum HP7-1 (Zhang et al, 2014), as well as the mutant P. oxalicum strain EU2106 (Huang et al, 2015), which were stocked in the China General Microbiological Collection Center, Beijing, China under numbers CGMCC 10781 and 6471, respectively. SB was a gift from the Nanning Sugar Factory, Nanning, China.…”

“…Enzymatic hydrolysis of USB of 80-mesh size by the crude enzyme solution (CES) from P. oxalicum EU2106 was conducted as described by Huang et al (2015). The enzyme loading was constant, with a solid loading of 25 filter paper units (FPU)/g solid.…”

“…Enzymatic hydrolyses were conducted using the CESs produced by the candidate strains, and HP7-1 as the reference strain, at the same substrate concentrations (6.0%, w/v) and amounts of enzyme (20 FPU/g biomass) by following the procedure of Huang et al (2015) and/or Zhang et al (2014), with some modifications. The hydrolysis experiments were conducted at 50 • C and pH 5.0 in a 200 rpm shaker for 96 h. Samples were withdrawn at various intervals.…”

“…The preparation of CESs produced by the isolates was performed according to the procedures described by Huang et al (2015). Briefly, the isolates were cultivated on potato dextrose agar plates at 28 • C for 6 days to induce sporulation.…”

“…Ideally, pretreatment should maximize lignin removal while keeping cellulose intact (Zeng et al, 2014). Previously, studies showed that plant species and/or varieties affected the pretreatment applications, resulting in a wide range of enzymatic hydrolysis efficiencies (Huang et al, 2015;Kumar et al, 2013;Li et al, 2014). Similarly, the conversion of SB into single sugars also cannot satisfactorily reach 100% as a result of the recalcitrance of the residues to enzymatic hydrolysis (Karp et al, 2013).…”