Efficient fermentable sugar production from mulberry branch based on a rational design of GH10 xylanase with improved thermal stability

Bai, Zhi-Yuan; You, Shuai; Zhang, Fang; Zhao, Dong; Zhao, Yifan; Wen, Hong-Jian; Wang, Jun

doi:10.1016/j.renene.2023.02.043

Cited by 10 publications

(2 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Enzymes can be expressed naturally or heterologously, depending on where the coding sequence has been located, to later be inserted into prokaryotes and ascomycetes [ 106 , 107 ]. Based on the above, an isolated, efficient, and abundant production is sought to reduce costs and time in results, for which mutation processes have also been generated, where the change of amino acids in the active site sometimes achieves a greater catalytic reaction speed, thermal stability, and tolerance to salinity, which allows them to continue active in catabolism or anabolism depending on the process to be carried out [ 108 , 109 , 110 ].…”

Section: Recent Trendsmentioning

confidence: 99%

Enzymatic Treatments for Biosolids: An Outlook and Recent Trends

Quintero-García

Pérez-Soler

Amezcua‐Allieri

2023

IJERPH

View full text Add to dashboard Cite

Wastewaters are nutrient-rich organic materials containing significant concentrations of different nutrients, dissolved and particulate matter, microorganisms, solids, heavy metals, and organic pollutants, including aromatic xenobiotics. This variety makes wastewater treatment a technological challenge. As a result of wastewater treatment, biosolids are generated. Biosolids, commonly called sewage sludge, result from treating and processing wastewater residuals. Increased biosolids, or activated sludge, from wastewater treatment is a major environmental and social problem. Therefore, sustainable and energy-efficient wastewater treatment systems must address the water crisis and environmental deterioration. Although research on wastewater has received increasing attention worldwide, the significance of biosolids treatments and valorization is still poorly understood in terms of obtaining value-added products. Hence, in this review, we established some leading technologies (physical, chemical, and biological) for biosolids pretreatment. Later, the research focuses on natural treatment by fungal enzymes to end with lignocellulosic materials and xenobiotic compounds (polyaromatic hydrocarbons) as a carbon source to obtain biobased chemicals. Finally, this review discussed some recent trends and promising renewable resources within the biorefinery approach for bio-waste conversion to value-added by-products.

show abstract

Section: Recent Trendsmentioning

confidence: 99%

Enzymatic Treatments for Biosolids: An Outlook and Recent Trends

Quintero-García

Pérez-Soler

Amezcua‐Allieri

2023

IJERPH

View full text Add to dashboard Cite

show abstract

“…Based on protein structure analysis, mutations at speci c amino acid sites have been demonstrated to signi cantly improve enzyme stability (25). For instance, the rational design of Xylanase Hwxyl10A from Hortaea werneckii led to a 17-fold improvement in thermostability at 50°C (3). The active pocket is a critical site for enzyme catalysis, and although the active site is highly conserved, changes in amino acids near the active site can also have a signi cant impact on enzyme activity and function, even if this relationship has not been reported (30).…”

Section: Introductionmentioning

confidence: 99%

Improving the catalytic properties of xylanase from Alteromones Macleadii H35 through evolution analysis

Cui,

xu,

et al. 2024

Preprint

View full text Add to dashboard Cite

Endo-1,4-β-xylanase is considered one of the most important xylanolytic enzymes, and in this study, we aimed to improve the catalytic properties of Alteromones Macleadii xylanase (Xyn ZT-2) using an evolution-guided design approach. Analysis of the amino acid sequence revealed that the amino acids located in close proximity to the active site were highly conserved, with only a few amino acid differences. By introducing various mutations, we were able to modify the catalytic performance of the enzyme. Notably, the A152G mutation resulted in a 9.8-fold increase in activity and a 23.2-fold increase in catalytic efficiency. Furthermore, the optimal temperature of A152G was raised to 65°C, which is 20°C higher than that of Xyn ZT-2, and the half-life period of T287S was enhanced by 4.9 times. These findings demonstrate the significance of amino acid evolution in determining the catalytic performance of xylanase. By utilizing an evolution analysis to create a smaller mutation library, we efficiently enhanced the catalytic performance, thus providing a novel strategy for improving enzyme catalytic efficiency.

show abstract

Improving the Catalytic Properties of Xylanase from Alteromones Macleadii H35 Through Sequence Analysis

Cui,

Xu,

et al. 2024

Appl Biochem Biotechnol

View full text Add to dashboard Cite

Efficient fermentable sugar production from mulberry branch based on a rational design of GH10 xylanase with improved thermal stability

Cited by 10 publications

References 43 publications

Enzymatic Treatments for Biosolids: An Outlook and Recent Trends

Enzymatic Treatments for Biosolids: An Outlook and Recent Trends

Improving the catalytic properties of xylanase from Alteromones Macleadii H35 through evolution analysis

Improving the Catalytic Properties of Xylanase from Alteromones Macleadii H35 Through Sequence Analysis

Contact Info

Product

Resources

About