Efficient filter-in-centrifuge separation of low-concentration bacteria from blood

Zeng, Kaiyang; Osaid, Mohammad; van der Wijngaart, Wouter

doi:10.1039/d3lc00594a

Cited by 5 publications

(3 citation statements)

References 36 publications

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“…5b). 215 The successful translation of biosensing devices into commercial POC diagnostics, relies on the successful integration of sample collection and processing with bioanalytical sensing, signal acquisition, and processing to improve the simplicity of assay operation by the end users.…”

Section: Device Integrationmentioning

confidence: 99%

Editors’ Choice—Challenges and Opportunities for Developing Electrochemical Biosensors with Commercialization Potential in the Point-of-Care Diagnostics Market

Akhlaghi,

Kaur,

Adhikari

et al. 2024

ECS Sens. Plus

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There is a plethora of electrochemical biosensors developed for ultrasensitive detection of clinically-relevant biomarkers. However, many of these systems lose their performance in heterogeneous clinical samples and are too complex to be operated by end users at the point-of-care (POC), prohibiting their commercial success. Integration of biosensors with sample processing technology addresses both of these challenges; however, it adds to the manufacturing complexity and the overall cost of these systems. Herein, we review the different components of a biosensor and avenues for creating fully-integrated systems. In the context of integration, we focus on discussing the trade-offs between sensing performance, cost, and scalable manufacturing to guide the readers toward designing new electrochemical biosensors with commercialization potential.

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Section: Device Integrationmentioning

confidence: 99%

Editors’ Choice—Challenges and Opportunities for Developing Electrochemical Biosensors with Commercialization Potential in the Point-of-Care Diagnostics Market

Akhlaghi,

Kaur,

Adhikari

et al. 2024

ECS Sens. Plus

View full text Add to dashboard Cite

show abstract

“…[ 13 ] To address these challenges, many methodologies have been developed to separate and concentrate target bacterial biomarkers from the sample matrix before detection. Techniques such as centrifugation, [ 14 ] magnetic beads, [ 15 ] membrane filters, [ 16 ] and increasingly popular microfluidic columns [ 17 ] have been employed for this purpose. However, the current separation and detection processes remain disjointed, leading to a significant increase in bacterial detection time.…”

Section: Introductionmentioning

confidence: 99%

Seamless Integration of Rapid Separation and Ultrasensitive Detection for Complex Biological Samples Using Multistage Annular Functionalized Carbon Nanotube Arrays

Li,

Zhang,

Jiao

et al. 2024

Advanced Materials

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Efficient separation, enrichment, and detection of bacteria in diverse media are pivotal for identifying bacterial diseases and their transmission pathways. However, conventional bacterial detection methods that split the separation and detection steps are plagued by prolonged processing times, high costs, and intricate procedures that hinder the advancement of pathogen detection techniques. Herein, we introduce a multistage annular‐functionalized carbon nanotube array device designed for the seamless integration of complex biological sample separation and multi‐marker detection. This device resorted to the super‐smooth fluidity of the liquid sample in the carbon nanotubes interstice through rotation assistance, achieving the ability to quickly separate impurities and capture biomarkers (1 mL sample cost time of 2.5 s). Fluid dynamics simulations showed that the reduction of near‐surface hydrodynamic resistance drives the capture of bacteria and related biomarkers on the functionalized surface of carbon nanotube in sufficient time. When further assembled as an even detection device, it exhibited fast detection (<30 min), robust linear correlation (101–107 colony‐forming units [CFU]/mL, R2 = 0.997), ultrasensitivity (limit of detection = 1.7 CFU/mL), and multi‐target detection (Staphylococcus aureus, extracellular vesicles, and enterotoxin proteins). Collectively, our material and system offer an expanded platform for real‐time diagnostics, enabling integrated rapid separation and detection of various disease biomarkers. The device has significant potential applications in fields such as emergent infectious disease diagnosis and treatment, infection source tracing, and environmental monitoring.This article is protected by copyright. All rights reserved

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“…Centrifugation combined with cross-flow filtration utilizes sedimentation velocity-based differentiation followed by size-based cross-flow filtration within a viscous solution. This is achieved through centrifugal sedimentation with an angled filter for bacterial passage [14]. However, this technique shares similar limitations, including culture-based detection, a high final sample volume (18 mL), and unknown compatibility with NAATs.…”

Section: Introductionmentioning

confidence: 99%

Facile Filtration-Based Workflow for Facilitating Sensitive Detection of Pathogenic Bacteria from Blood

Park,

Chen,

et al. 2023

Preprint

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Bloodstream infections (BSIs) are a global health concern, contributing to high mortality rates and increased healthcare costs. Current diagnostic methods, such as blood culture, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), immunoassays, and nucleic acid amplification tests (NAATs), have lengthy workflow bottlenecked by the need of culture based method This study introduces an innovative blood processing method that overcomes this limitation, enabling rapid and culture-free isolation of pathogenic bacteria from whole blood. The method combines osmolysis, blood separation membrane filtration, detergent and enzymatic lysis, and bacterial capture, requiring minimal specialized equipment. Notably, it uses a small blood sample volume (0.5 mL or below), making it suitable for pediatric patients. The workflow involves four simple steps and can be completed in approximately 30 minutes, providing rapid pathogen isolation. The protocol was successfully tested with two most common causative bacteria, S. aureus and E. coli, achieving sensitive detection down to approximately 10 CFU using benchtop PCR. The culture-free approach accelerates the diagnosis process and minimizes the risk of bacterial population alterations during culturing. While further optimization and testing with a broader range of pathogens are needed, this method holds promise in advancing the diagnosis of life-threatening bacterial infections. Future applications may include rapid point-of-care testing in resource-limited settings.

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Efficient filter-in-centrifuge separation of low-concentration bacteria from blood

Abstract: Septic blood centrifugation over an inclined filter efficiently separates bacteria from blood cells at clinically relevant concentrations.

Cited by 5 publications

References 36 publications

Editors’ Choice—Challenges and Opportunities for Developing Electrochemical Biosensors with Commercialization Potential in the Point-of-Care Diagnostics Market

Editors’ Choice—Challenges and Opportunities for Developing Electrochemical Biosensors with Commercialization Potential in the Point-of-Care Diagnostics Market

Seamless Integration of Rapid Separation and Ultrasensitive Detection for Complex Biological Samples Using Multistage Annular Functionalized Carbon Nanotube Arrays

Facile Filtration-Based Workflow for Facilitating Sensitive Detection of Pathogenic Bacteria from Blood

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