Efficient gene delivery of primary human cells using peptide linked polyethylenimine polymer hybrid

Dey, Devaveena; Inayathullah, Mohammed; Lee, Andrew S.; LeMieux, Melburne C.; Zhang, Xuexiang; Wu, Yi; Nag, Divya; Almeida, Patricia E. de; Han, Leng; Rajadas, Jayakumar; Wu, Joseph C.

doi:10.1016/j.biomaterials.2011.03.016

Cited by 58 publications

(40 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To investigate if our carrier system provided physical protection of the complexed DNA against intracellular DNases, a stabilization assay was used . DNases are ubiquitously present in cells mostly in the nucleus and take part in many cellular activities concerned with DNA processing such as DNA repair and apoptosis .…”

Section: Resultsmentioning

confidence: 99%

A Systematic Structure–Activity Study of a New Type of Small Peptidic Transfection Vector Reveals the Importance of a Special Oxo‐Anion‐Binding Motif for Gene Delivery

et al. 2017

View full text Add to dashboard Cite

We discovered a new class of artificial peptidic transfection vectors based on an artificial anion-binding motif, the guanidiniocarbonylpyrrole (GCP) cation. This new type of vector is surprisingly smaller than traditional systems, and our previous work suggested that the GCP group was important for promoting critical endosomal escape. We now present here a systematic comparison of similar DNA ligands featuring our GCP oxo-anion-binding motif with DNA ligands only consisting of naturally occurring amino acids. Structure-activity studies showed that the artificial binding motif clearly outperformed natural amino acids such as histidine, lysine, and arginine. It improved the ability to shuttle foreign genetic material into cells, yet successfully mediated endosomal escape. Also, plasmids that were complexed by our artificial ligands were stabilized against cytosolic degradation to some extent. This resulted in the successful expression of plasmid information (comparable to gold standards such as polyethyleneimine). Hence, our study clearly demonstrates the importance of the tailor-made GCP anion-binding site for efficient gene transfection.

show abstract

Section: Resultsmentioning

confidence: 99%

A Systematic Structure–Activity Study of a New Type of Small Peptidic Transfection Vector Reveals the Importance of a Special Oxo‐Anion‐Binding Motif for Gene Delivery

et al. 2017

View full text Add to dashboard Cite

show abstract

“…On the other hand, upon crosslinking PEI with GDE, the most distinct peaks are 1051 and 1109 cm −1 coming from alcohol groups, for O‐H bending and C‐O stretching from secondary alcohol, respectively. PEI cryogels were also treated with 200 mL of 1.0 M NaOH, and 0.1 M HCl for 4 h assess the generated peaks as given in Figures (a) and , respectively, and the corresponding peaks are shown in the figure. The quarternized amine peaks are clearly seen at 1660 cm −1 for all the PEI materials.…”

Section: Resultsmentioning

confidence: 99%

“…Cationic polymers are very attractive for numerous applications, such as drug delivery systems, gene delivery, environmental applications, and so on. Amine‐based cationic polymers, especially, are very resourceful due to their modifiable nature .…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, among the amine‐based polymers PEI has drawn special attention due to its superior properties for DNA transfection and CO 2 capture . PEI possesses the highest DNA transfection capability due to its relatively efficient ability to complex with higher amounts of DNA . In recent years, materials derived from PEI have been investigated for CO 2 absorption .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Poly ionic liquid cryogel of polyethyleneimine: Synthesis, characterization, and testing in absorption studies

Şahiner

Demirci

2016

J of Applied Polymer Sci

View full text Add to dashboard Cite

Here, we report the synthesis of polyethyleneimine (PEI) cryogels for the first time via cryopolymerization technique. The crosslinking of amine groups on the branched PEI chains is accomplished with epoxy groups of glycerol diglycidyl ether (GDE) based on epoxy-amine reactions in excess water at 218 8C in about 16 h. Superporous PEI cryogels with pore sizes >100 lm were shown to have very fast equilibrium swelling behavior, e.g., 10 s to reach maximum swelling in DI water. Furthermore, the synthesized PEI cryogels were exposed to anion exchange reaction after protonation by HCl treatments to generate PEI ionic liquid cryogels containing hexafluorophosphate, thiocyanate, dicyanamide, and tetrafluoroborate. It was also demonstrated that PEI cryogels modified with [PF 6 ] 2 absorbed 47.8 6 5.7 mg/g of bovine serum albumin (BSA). Moreover, PEI cryogels were shown to be very useful as simple filtration filling materials for the direct removal of organic dyes such as methyl orange (MO) and eosin Y (EY) from their corresponding aqueous solutions with 98.5 and 98.6% yields, respectively. The separation of methylene blue (MB) from MO and EY mixture by using PEI cryogels as column filler materials was also demonstrated.

show abstract

“…Guanidinylation or addition of α-tocopherol succinate enhanced the cellular uptake of chitosan complexes [46,47], whereas stearoyl groups facilitate cell penetration of polypeptides [48]. Some peptides have a natural cell penetration capability which can be linked to other molecular structures [49].…”

mentioning

confidence: 99%

Vehicles for Small Interfering RNA transfection: Exosomes versus Synthetic Nanocarriers

Duechler¹

2013

DNA and RNA Nanotechnology

View full text Add to dashboard Cite

Therapies based on RNA interference (RNAi) hold a great potential for targeted interference of the expression of specific genes. Small-interfering RNAs (siRNA) and micro-RNAs interrupt protein synthesis by inducing the degradation of messenger RNAs or by blocking their translation. RNAibased therapies can modulate the expression of otherwise undruggable target proteins. Full exploitation of RNAi for medical purposes depends on efficient and safe methods for delivery of small RNAs to the target cells. Tremendous effort has gone into the development of synthetic carriers to meet all requirements for efficient delivery of nucleic acids into particular tissues. Recently, exosomes unveiled their function as a natural communication system which can be utilized for the transport of small RNAs into target cells. In this review, the capabilities of exosomes as delivery vehicles for small RNAs are compared to synthetic carrier systems. The step by step requirements for efficient transfection are considered: production of the vehicle, RNA loading, protection against degradation, lack of immunogenicity, targeting possibilities, cellular uptake, cytotoxicity, RNA release into the cytoplasm and gene silencing efficiency. An exosomebased siRNA delivery system shows many advantages over conventional transfection agents, however, some crucial issues need further optimization before broad clinical application can be realized.

show abstract

Efficient gene delivery of primary human cells using peptide linked polyethylenimine polymer hybrid

Cited by 58 publications

References 28 publications

A Systematic Structure–Activity Study of a New Type of Small Peptidic Transfection Vector Reveals the Importance of a Special Oxo‐Anion‐Binding Motif for Gene Delivery

A Systematic Structure–Activity Study of a New Type of Small Peptidic Transfection Vector Reveals the Importance of a Special Oxo‐Anion‐Binding Motif for Gene Delivery

Poly ionic liquid cryogel of polyethyleneimine: Synthesis, characterization, and testing in absorption studies

Vehicles for Small Interfering RNA transfection: Exosomes versus Synthetic Nanocarriers

Contact Info

Product

Resources

About