Efficient gene transfection of suspension cells by highly branched poly(β-amino ester)

Biomaterials as a new option for treating sensorineural hearing loss

“…They designed and synthesized highly branched poly(β-amino ester) (HPAE) using an "A2 + B4 + C2" Michael addition strategy, and successfully applied it to gene transfection in mast cells. 132 Compared to other non-viral transfection methods, this approach achieved over 80 times higher transfection efficiency. However, this method has only been validated in suspension cells so far and has yet to be tested in adherent cells such as hair cells.…”

Nanotechnology‐based CRISPR/Cas9 delivery system for genome editing in cancer treatment

“…Poly(β-amino ester) (PBAE) is another popular cationic polymeric genetic vehicle with strong biological compatibility. [188][189][190][191][192][193][194] Given that compared with their equivalent linear counterparts, branched polymers exhibit higher levels of gene transfection, 195 Wang et al connected the linear PBAE with branching units to obtain a new multifunctional linear-branched hybrid PBAE (LBPAE). 196 The gene transfection efficiency of LBPAE reached 94% in human primary dermal fibroblasts and 91% in mouse embryo fibroblasts, significantly superior to commercial reagents PEI and SuperFect, and the fluorescence intensity was an additional order of magnitude greater compared to these two commercialized transfection substances.…”

“…Poly(β‐amino ester) (PBAE) is another popular cationic polymeric genetic vehicle with strong biological compatibility 188–194 . Given that compared with their equivalent linear counterparts, branched polymers exhibit higher levels of gene transfection, 195 Wang et al connected the linear PBAE with branching units to obtain a new multifunctional linear‐branched hybrid PBAE (LBPAE) 196 .…”