Efficient generation of TBX3+ atrioventricular conduction-like cardiomyocytes from human pluripotent stem cells

Du, Ru Xu; Bai, Shuyun; Zhao, Yang

doi:10.1016/j.bbrc.2023.05.104

Cited by 3 publications

(1 citation statement)

References 21 publications

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“…Recently, three-dimensional applications of pacing cells have been carried out in vitro ( 28 , 29 ). In addition to the sinoatrial node, other cells of the cardiac conduction system have been generated, such as atrioventricular node-like cells ( 30 ) or cell lines that help to decipher the respective structure ( 31 ). Recently, there has been a focus on Purkinje-like cell differentiation ( 32 – 34 ).…”

Section: Introductionmentioning

confidence: 99%

Heart rhythm in vitro: measuring stem cell-derived pacemaker cells on microelectrode arrays

Kussauer,

Dilk,

Elleisy

et al. 2024

Front. Cardiovasc. Med.

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BackgroundCardiac arrhythmias have markedly increased in recent decades, highlighting the urgent need for appropriate test systems to evaluate the efficacy and safety of new pharmaceuticals and the potential side effects of established drugs.MethodsThe Microelectrode Array (MEA) system may be a suitable option, as it provides both real-time and non-invasive monitoring of cellular networks of spontaneously active cells. However, there is currently no commercially available cell source to apply this technology in the context of the cardiac conduction system (CCS). In response to this problem, our group has previously developed a protocol for the generation of pure functional cardiac pacemaker cells from mouse embryonic stem cells (ESCs). In addition, we compared the hanging drop method, which was previously utilized, with spherical plate-derived embryoid bodies (EBs) and the pacemaker cells that are differentiated from these.ResultsWe described the application of these pacemaker cells on the MEA platform, which required a number of crucial optimization steps in terms of coating, dissociation, and cell density. As a result, we were able to generate a monolayer of pure pacemaker cells on an MEA surface that is viable and electromechanically active for weeks. Furthermore, we introduced spherical plates as a convenient and scalable method to be applied for the production of induced sinoatrial bodies.ConclusionWe provide a tool to transfer modeling and analysis of cardiac rhythm diseases to the cell culture dish. Our system allows answering CCS-related queries within a cellular network, both under baseline conditions and post-drug exposure in a reliable and affordable manner. Ultimately, our approach may provide valuable guidance not only for cardiac pacemaker cells but also for the generation of an MEA test platform using other sensitive non-proliferating cell types.

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Section: Introductionmentioning

confidence: 99%

Heart rhythm in vitro: measuring stem cell-derived pacemaker cells on microelectrode arrays

Kussauer,

Dilk,

Elleisy

et al. 2024

Front. Cardiovasc. Med.

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Modeling the atrioventricular conduction axis using human pluripotent stem cell-derived cardiac assembloids

Li,

Wiesinger,

Fokkert

et al. 2024

Cell Stem Cell

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Genes Selectively Expressed in Rat Organs

Li,

Wan,

Yun

et al. 2024

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Background: Understanding organic functions at a molecular level is important for scientists to unveil the disease mechanism and to develop diagnostic or therapeutic methods. background: Understanding organic functions at a molecular level are important for scientists to unveil the mechanism of disease and to develop diagnostic or therapeutic methods. Aim: The present study tried to find genes selectively expressed in 11 rat organs, including the adrenal gland, brain, colon, duodenum, heart, ileum, kidney, liver, lung, spleen, and stomach. objective: Understanding organic functions at a molecular level are important for scientists to unveil the mechanism of disease and to develop diagnostic or therapeutic methods. The present study tried to find genes selectively expressed in 11 rat organs, including the adrenal gland, brain, colon, duodenum, heart, ileum, kidney, liver, lung, spleen, and stomach. Method: Three normal male Sprague-Dawley (SD) rats were anesthetized, their organs mentioned above were harvested, and RNA in the fresh organs was extracted. Purified RNA was reversely transcribed and sequenced using the Solexa high-throughput sequencing technique. The abundance of a gene was measured by the expected value of fragments per kilobase of transcript sequence per million base pairs sequenced (FPKM). Genes in organs with the highest expression level were sought out and compared with their median value in organs. If a gene in the highest expressed organ was significantly different (p < 0.05) from that in the medianly expressed organ, accompanied by q value < 0.05, and accounted for more than 70% of the total abundance, the gene was assumed as the selective gene in the organ. Results & Discussion: The Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene Ontology (GO) pathways were enriched by the highest expressed genes. Based on the criterion, 1,406 selective genes were screened out, 1,283 of which were described in the gene bank and 123 of which were waiting to be described. KEGG and GO pathways in the organs were partly confirmed by the known understandings and a good portion of the pathways needed further investigation. Conclusion: The novel selective genes and organic functional pathways are useful for scientists to unveil the mechanisms of the organs at the molecular level, and the selective genes’ products are candidate disease markers for organs.

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Efficient generation of TBX3+ atrioventricular conduction-like cardiomyocytes from human pluripotent stem cells

Cited by 3 publications

References 21 publications

Heart rhythm in vitro: measuring stem cell-derived pacemaker cells on microelectrode arrays

Heart rhythm in vitro: measuring stem cell-derived pacemaker cells on microelectrode arrays

Modeling the atrioventricular conduction axis using human pluripotent stem cell-derived cardiac assembloids

Genes Selectively Expressed in Rat Organs

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