2018
DOI: 10.1101/465286
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Efficient GFP-labeling and analysis of spermatogenic cells using the IRG transgene and flow cytometry

Abstract: Spermatogenesis is a highly ordered developmental program that produces haploid male germ cells. The study of male germ cell development in the mouse has provided unique perspectives into the molecular mechanisms that control cell development and differentiation in mammals, including tissue-specific gene regulatoryprograms. An intrinsic challenge in spermatogenesis research is the heterogeneity of germ and somatic cell types present in the testis. Techniques to separate and isolate distinct mouse spermatogenic… Show more

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