Efficient hybridization between Lycopersicon esculentum and L. peruvianum via embryo callus

Thomas, Bruce R.; Pratt, David

doi:10.1007/bf00265495

Cited by 83 publications

(31 citation statements)

References 10 publications

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“…Embryo of hybrid L. esculenturn x L. peruvianurn was excised and cultured in MS medium added with kinetin and NAA. Experiments conducted have indicated that the embryo callus culture is more efficient than embryo culture (Thomas and Pratt 1981;Wu 1984). Experiments conducted have indicated that the embryo callus culture is more efficient than embryo culture (Thomas and Pratt 1981;Wu 1984).…”

Section: Overcoming the Barriersmentioning

confidence: 99%

Genetic Improvement of Tomato

Kalloo¹

1991

Monographs on Theoretical and Applied Genetics

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Section: Overcoming the Barriersmentioning

confidence: 99%

Genetic Improvement of Tomato

Kalloo¹

1991

Monographs on Theoretical and Applied Genetics

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“…These procedures are also directed toward obtaining more efficient survival of embryos in situations where very immature embryos are to be cultured . Wide crosses in tomato which did not produce viable seeds, could be obtained through embryo callus production and subsequent regeneration and rooting of the callus (Thomas & Pratt, 1981) . The possibility of increasing crossability also exists by pre-disposing crop embryos to alien endosperm and then using plants raised from those embryos to cross with the alien species.…”

Section: Unconventional Reciprocal Crosses As Means To Successmentioning

confidence: 99%

How wide can a wide cross be?

Sharma

1995

Euphytica

100

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Wide crosses in wheat are reviewed in relation to various factors that facilitate wide crossing to show that wide crosses can be as wide as one can make them . Included in this review is a particular reference to wheat-wheatgrass (Agropyron complex) crosses and an update on wide crosses of wheat with various genera of Agropyron complex . Hybrid seed set is too variable to predict whether a wide hybrid, where no seed was obtained in one attempt, will not be possible. High crossability genes seem to facilitate not only fertilization but also seed development, enabling embryo rescue . Variability for crossability occurs not only in wheat but also in alien species . Contrary to conventional thinking, several wide hybrids with wheat can be produced when species with lower chromosome numbers are used as female parents . Pre-and post-fertilization barriers to wide crosses do not appear to be equally strong, and can be overcome by the development and application of various technologies . Considering these aspects of wide hybridization, and based on recent successes in producing previously unsuccessful and very wide hybrids, it is concluded that how wide cross between plant species can be made is an open question and that many new and wider hybrids can be produced in future.

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“…Minicalli were transferred to solidified TMcp~Z greening medium (Wolters et al 1991) and subcultured every month. Larger calli were transferred to 1Z medium (modified 2Z medium (Thomas & Pratt 1981) with 1 instead of 2 mg 1-1 zeatin) for faster growth and regeneration.…”

Section: Isolation Fusion and Culture Of Protoplastsmentioning

confidence: 99%

Use of leaky nitrate reductase-deficient mutants of tomato (Lycopersicon esculentum Mill.) for selection of somatic hybrid cell lines with wild type potato (Solanum tuberosum L.)

Schoenmakers

Nobel

Koornneef

1992

Plant Cell Tiss Organ Cult

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Protoplasts of two 'leaky' nitrate reductase deficient and thus nitrate auxotrophic (NAR) mutants of tomato and their wild types, were fused with protoplasts of monoploid potato. In all four combinations hybrid calli grew more vigorously than parental calli and this somatic hybrid vigour as such provided a useful enrichment for somatic hybrids. Selection against nitrate auxotrophy further increased the efficiency of the enrichment, particularly if a molybdenum cofactor mutation was used as the basis for the selection. It is concluded that the nitrate auxotrophy of these NAR mutants is sufficiently expressed at the level of the cell, to allow its use in somatic hybridization experiments with potato.

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Efficient hybridization between Lycopersicon esculentum and L. peruvianum via embryo callus

Cited by 83 publications

References 10 publications

Genetic Improvement of Tomato

Genetic Improvement of Tomato

How wide can a wide cross be?

Use of leaky nitrate reductase-deficient mutants of tomato (Lycopersicon esculentum Mill.) for selection of somatic hybrid cell lines with wild type potato (Solanum tuberosum L.)

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