2012
DOI: 10.5504/bbeq.2012.0049
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EfficientIn VitroRegeneration of Sugarcane (Saccharum OfficinarumL.) from Bud Explants

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Cited by 8 publications
(4 citation statements)
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“…With respect to root induction, 25% of shoot clusters of LSC formed well-defined roots spontaneously on BAPcontaining medium (SPM) after 4 weeks of culture compared to 57% in B36464 shoot clusters (Figure 4C). This phenomenon of spontaneous root formation at the shoot maturation stage on media containing only cytokinins has been reported by Zamir et al (2012). Nonetheless, to improve root formation from in vitro shoots, we supplemented MS media with 3 mg/L NAA which resulted in marked improvement in root development in both B36464 (88%) and LSC (72%) after 8 weeks of culture.…”
Section: Shoot Proliferation and Root Inductionsupporting
confidence: 54%
“…With respect to root induction, 25% of shoot clusters of LSC formed well-defined roots spontaneously on BAPcontaining medium (SPM) after 4 weeks of culture compared to 57% in B36464 shoot clusters (Figure 4C). This phenomenon of spontaneous root formation at the shoot maturation stage on media containing only cytokinins has been reported by Zamir et al (2012). Nonetheless, to improve root formation from in vitro shoots, we supplemented MS media with 3 mg/L NAA which resulted in marked improvement in root development in both B36464 (88%) and LSC (72%) after 8 weeks of culture.…”
Section: Shoot Proliferation and Root Inductionsupporting
confidence: 54%
“…In contrast, somatic embryos were reported to be induced at lower concentrations of 2,4-D (1 mg/l), whereas higher concentrations induced non-embryogenic calli (Zamir et al, 2014). Sequential removal of 2,4-D followed by sub-culture on MS supplemented with 2mg/l BAP induced 98% shoot induction (after 3 weeks of culture) with maximum shoot elongation (9.4 cm) (Zamir et al, 2014). Similarly, callus induced in MS + 3.0 mg/l 2,4-D and subsequently incubated on 2,4-D-free media was found to be commercially viable for plantlet regeneration on MS + 1.0-1.5 mg/l BAP + 0.2 mg/l NAA in elite sugarcane genotypes (Abdu et al, 2012).…”
Section: Response To In Vitro Culturementioning
confidence: 92%
“…Khamrit et al (2012) reported maximum percentage of somatic embryogenic callus induction in MS medium supplemented with 3 mg/l 2,4-D and 15% (v/v) coconut water. In contrast, somatic embryos were reported to be induced at lower concentrations of 2,4-D (1 mg/l), whereas higher concentrations induced non-embryogenic calli (Zamir et al, 2014). Sequential removal of 2,4-D followed by sub-culture on MS supplemented with 2mg/l BAP induced 98% shoot induction (after 3 weeks of culture) with maximum shoot elongation (9.4 cm) (Zamir et al, 2014).…”
Section: Response To In Vitro Culturementioning
confidence: 99%
“…In Pakistan, this pathogen first reported in 1986 by Ahmed et al, 1986. The major reason of common of red rot disease in Pakistan is due to cultivation of susceptible Sugarcane varieties that difficult to incorporate the new sugarcane cultivars with developed agronomic features and resistance to biotic and abiotic stresses (Zamir et al, 2012). Red rot of sugarcane may infect developed stems of cane, leaf mid ribs which results in considerable damages in sugar quality (Rao et al, 2008).…”
Section: Introductionmentioning
confidence: 99%