Efficient imaging based on P - and N-codoped carbon dots for tracking division and viability assessment of lactic acid bacteria

Fu, Tianxin; Yang, Wenjun; Jin, Fu; Liu, Buwei; Wang, Jindi; Yin, Xinyue; Fu, Xiangbo; Tian, Bingbing; Zhang, Feng

doi:10.1016/j.colsurfb.2023.113155

Cited by 4 publications

(4 citation statements)

References 43 publications

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“…Alternative mechanisms might involve interaction of CDs with the peptidoglycan and glucose transporter proteins (GTPs) of the HILP cell membrane. The abundant hydrophilic groups on the peptidoglycan cell wall component of HILP may interact with the hydroxyl groups on the surface of CDs via hydrogen bonding ( Fu et al, 2023 ). Moreover, uptake of CDs by LP may involve the interaction of CDs bearing intrinsically acquired surface glucose functional groups with the glucose transporter proteins (GTPs) of the LP cell membrane.…”

Section: Resultsmentioning

confidence: 99%

“…CDs are a relatively new type of fluorescent nanomaterials with excellent photoluminescence ( Jing et al, 2023 ). Indeed, CDs labeling is a versatile, simple, and inexpensive technique of value in selective biolabeling of bacteria, tumor cells, proteins, and subcellular organelles for a wide range of bioimaging and theranostic purposes ( Hallaji et al, 2022 ; Phan and Cho, 2022 ; Fu et al, 2023 ). Moreover, CDs were widely investigated as fluorescent carriers for targeted drug delivery ( Boakye-Yiadom et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

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Carbon dots labeled Lactiplantibacillus plantarum: a fluorescent multifunctional biocarrier for anticancer drug delivery

Abdullah

Mahmoud

El-Nikhely

et al. 2023

Front. Bioeng. Biotechnol.

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A carbon dots (CDs)-biolabeled heat-inactivated Lactiplantibacillus plantarum (HILP) hybrid was investigated as a multifunctional probiotic drug carrier with bioimaging properties using prodigiosin (PG) as anticancer agent. HILP, CDs and PG were prepared and characterized using standard methods. CDs-labeled HILP (CDs/HILP) and PG loaded CDs/HILP were characterized by transmission electron microscopy (TEM), laser scanning confocal microscopy (LSCM) and for entrapment efficiency (EE%) of CDs and PG, respectively. PG-CDs/HILP was examined for stability and PG release. the anticancer activity of PG-CDs/HILP was assessed using different methods. CDs imparted green fluorescence to HILP cells and induced their aggregation. HILP internalized CDs via membrane proteins, forming a biostructure with retained fluorescence in PBS for 3 months at 4°C. Loading PG into CDs/HILP generated a stable green/red bicolor fluorescent combination permitting tracking of both drug carrier and cargo. Cytotoxicity assay using Caco-2 and A549 cells revealed enhanced PG activity by CDs/HILP. LCSM imaging of PG-CDs/HILP-treated Caco-2 cells demonstrated improved cytoplasmic and nuclear distribution of PG and nuclear delivery of CDs. CDs/HILP promoted PG-induced late apoptosis of Caco-2 cells and reduced their migratory ability as affirmed by flow cytometry and scratch assay, respectively. Molecular docking indicated PG interaction with mitogenic molecules involved in cell proliferation and growth regulation. Thus, CDs/HILP offers great promise as an innovative multifunctional nanobiotechnological biocarrier for anticancer drug delivery. This hybrid delivery vehicle merges the physiological activity, cytocompatibility, biotargetability and sustainability of probiotics and the bioimaging and therapeutic potential of CDs.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Carbon dots labeled Lactiplantibacillus plantarum: a fluorescent multifunctional biocarrier for anticancer drug delivery

Abdullah

Mahmoud

El-Nikhely

et al. 2023

Front. Bioeng. Biotechnol.

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“…13 It has been proved that heteroatom doping is an effective strategy to control surface defects, adjust electron energy levels, and change optical properties of CDs. 14 For example, nitrogen (N), as a typical dopant, can change the surface states of CDs, thereby increasing the QY and improving sensitivity of analytical detection. 15 Liao et al used hexamethylene tetramine as the C and N source to synthesize N-doped CDs (N-CDs) by one-step hydrothermal method, which was used as the fluorescent probes to detect Cu 2þ and glutathione, with the QY of 21.7%.…”

Section: Introductionmentioning

confidence: 99%

“…However, the relatively low quantum yield (QY) limits their practical application 13 . It has been proved that heteroatom doping is an effective strategy to control surface defects, adjust electron energy levels, and change optical properties of CDs 14 . For example, nitrogen (N), as a typical dopant, can change the surface states of CDs, thereby increasing the QY and improving sensitivity of analytical detection 15 .…”

Section: Introductionmentioning

confidence: 99%

Preparation of high quantum yield nitrogen and sulfur co-doped carbon dots for sensitive detection of Cu2+

Wang,

Cheng,

et al. 2024

J. Nanophoton.

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Nitrogen and sulfur co-doped green fluorescent carbon dots (G-CDs) with quantum yield (QY) of 28.26% were synthesized by hydrothermal method with p-phenylenediamine and thiosemicarbazone as precursors. The synthesized G-CDs exhibit good sensitivity and anti-interference ability for detecting copper ion (Cu 2þ ) by fluorescence quenching (λ ex ¼ 390 nm, λ em ¼ 535 nm) with a linear range of 20 to 200 μM and the limit of detection of 4.8 μM. This analytical method has been applied to detect Cu 2þ of the local lake with good accuracy and recovery.

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Fluorescent carbon dots for labeling of bacteria: mechanism and prospects—a review

Anand,

Huang,

Lai

et al. 2024

Anal Bioanal Chem

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Efficient imaging based on P - and N-codoped carbon dots for tracking division and viability assessment of lactic acid bacteria

Cited by 4 publications

References 43 publications

Carbon dots labeled Lactiplantibacillus plantarum: a fluorescent multifunctional biocarrier for anticancer drug delivery

Carbon dots labeled Lactiplantibacillus plantarum: a fluorescent multifunctional biocarrier for anticancer drug delivery

Preparation of high quantum yield nitrogen and sulfur co-doped carbon dots for sensitive detection of Cu2+

Fluorescent carbon dots for labeling of bacteria: mechanism and prospects—a review

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