Efficient immobilization of acetylcholinesterase onto amino functionalized carbon nanotubes for the fabrication of high sensitive organophosphorus pesticides biosensors

Yu, Guangxia; Wu, Weixiang; Zhao, Qiang; Wei, Xiaoyun; Lü, Qing

doi:10.1016/j.bios.2015.01.005

Cited by 137 publications

(47 citation statements)

References 33 publications

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“…Bionanosensors also makes it possible for rapid detection of bacteria and viruses with precise quantification and thereby increasing the food safety for the customer [137]. A highly sensitive organophosphorus pesticide biosensor constructed with the help of surface functionalized carbon nanotubes tailored with amino groups to control the efficient immobilization of acetylcholinesterase onto the surface of glassy carbon electrode and these have been successfully used for the direct analysis of vegetable samples [138]. An acetyl cholinesterase biosensor based on assembly of multiwall carbon nanotubes onto liposome bioreactors was developed for detection of organophosphates pesticides [139].…”

Section: Applications Of Nanotechnology In Precision Agriculturementioning

confidence: 99%

Nanotechnology: The new perspective in precision agriculture

Duhan

Kumar

et al. 2017

Biotechnology Reports

925

316

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HighlightsPrecision farming is measuring and responding to inter and intra-field variability in crops to form a decision support system.About 40–70% of N, 80–90% of P and 50–70% of K of the applied fertilizers is lost to the environment causing pollution.Nanofertilizers helps in slow and sustained release of agrochemicals resulting in precise dosage to the plants.Green synthesized Ag, ZnO and TiO2 NPs are extensively used for plant protection and treatment of diseases.Biosensors helps in detecting pesticides in the vegetable crops and form a decision support system for crop commodities.

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Section: Applications Of Nanotechnology In Precision Agriculturementioning

confidence: 99%

Nanotechnology: The new perspective in precision agriculture

Duhan

Kumar

et al. 2017

Biotechnology Reports

925

316

View full text Add to dashboard Cite

show abstract

“…This indicates higher affinity of the enzyme to the substrate, which can be caused by favorable microenvironment. Some other AChE biosensors with carbonaceous nanomaterials showed similar characteristics: 0.13 mM (AChE in the matrix of nafion, carboxylated graphene and silver nanoparticles [34]), 0.22 mM (TiO 2 decorated graphene [35]), 0.067 mM (immobilization on aminated carbon nanotubes [36]), and 0.135 mM (NiO nanoparticles in carboxylated graphene-nafion matrix [37]). …”

Section: Chronoamperometric Measurementsmentioning

confidence: 89%

Acetylcholinesterase biosensor for inhibitor measurements based on glassy carbon electrode modified with carbon black and pillar[5]arene

Shamagsumova

Shurpik

Padnya

et al. 2015

Talanta

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“…For example, Yu et al. 42 synthesized amino‐ functionalized CNT for the efficient immobilization of AChE and used for sensitive detection of paraoxon. Figure 2A and B illustrate the schematic representation of amination process of carbon nanotubes followed by efficient immobilization of AChE onto the CNTNH 2 modified glassy carbon electrode (GCE).…”

Section: Electrochemical Detection Of Nerve Agentmentioning

confidence: 99%

Recent Advances in Electrochemical Sensors for Detecting Weapons of Mass Destruction. A Review

Singh

2016

Electroanalysis

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1IntroductionChemical warfare agents (CWA) are toxic chemicals which have been deliberately used since World WarI [1]. Along with biological and nuclear threats;t hese weapons have become some of the most feared and least predictable mass destruction agentso nt he battlefield and in the hands of terrorists.A mong CWA, nerve agents,o ften organophosphates (OP), are ac lass of lethal weapons of mass destruction (WMD) that kill mankind by disrupting the nerve transfer mechanism [2][3].T he extreme toxicity of OP compounds is attributed to the inhibitiono ft he enzyme acetylcholinesterase (AChE)b yc ovalently blocking the active site of the enzyme which leadstoaccumulation of acetylcholine causing cholinergic over stimulation, culminating in death causedb yr espiratory failure [4].A n example of their potency, sarin was released by terrorists in aT okyo subways tation in 1995, resulting in more than 5000 humancasualties [5].Unfortunately,these highly adhesive and volatile nerve agents are colorless,o dorless and tasteless,m aking detection very difficult. Thed etermination of OP or nervea gents has become increasingly important for soldiers in war fielda sw ell as civilians due to the upsurge in terrorist activity [6].E arly detection of these noxiousO Pn eurotoxins is imperative for protecting water resources and food supplies,i nt he defense against terrorist activity,a nd for monitoring detoxification processes [6].C urrently,t here are many laboratory-based conventional analytical detection techniques for detecting OP compounds such as gas chromatography,h igh performance liquid chromatography,l iquidc hromatograph with mass spectrometer (LC-MS), and nuclear magnetic resonance (NMR) spectrometer [7][8][9][10].H owever, these techniques are time-consuming and expensive, non-portable and require aq ualified and experienced staff,a nd cannot be used for continuous monitoring [11][12][13][14].C onsidering the urgency of the situationt he CWAm ay cause,c ombatant therefore needs portable,e asiness of operation and ability to precisely detect thesea gents in real war field conditions.T he rapidd etection of WMD is also of pivotal importancet oh omelands ecurityi nt erms of responding to or recovering from the worst possible terrorist attack. Recent insights into the development of smart sensors and resultant analytical systems that are portable,l ightweight, accuratea nd low cost able to augmentt he use of these sensors onsite,t herebyp roviding added dimensions of rich,a nalyticali nformation to the combatant in at imely manner.T herefore,asimple and rapid yet reliable nerve agent detection warning system is critically desirable in the current climate of terrorism awareness.This review describes documented currentand developing technologies for electrochemical detection and identificationo fCWA and addresses the challenges associated with CWAd etection in complex sample matrices.H erein, we address sed about the electrochemical methodologies based on enzymatic and non-enzymatic principals for detection of nerve agents...

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Efficient immobilization of acetylcholinesterase onto amino functionalized carbon nanotubes for the fabrication of high sensitive organophosphorus pesticides biosensors

Cited by 137 publications

References 33 publications

Nanotechnology: The new perspective in precision agriculture

Nanotechnology: The new perspective in precision agriculture

Acetylcholinesterase biosensor for inhibitor measurements based on glassy carbon electrode modified with carbon black and pillar[5]arene

Recent Advances in Electrochemical Sensors for Detecting Weapons of Mass Destruction. A Review

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