Efficient In Vitro Plant Regeneration from Internode Explants of Ibervillea sonorae: An Antidiabetic Medicinal Plant

Abstract

<jats:p><jats:italic>Ibervillea sonorae</jats:italic> is a medicinal plant mainly used to treat diabetes, ulcers, and other metabolic disorders. A regeneration protocol using internode segments containing axillary buds grown on Gamborg medium (B5) supplemented with 0.5 mg·L<jats:sup>−1</jats:sup> α-naphthalene-acetic acid (NAA), 0.5 mg·L<jats:sup>−1</jats:sup> N6-benzyladenine (BA), and 1.0 mg·L<jats:sup>−1</jats:sup> indole-3-acetic acid (IAA) successfully regenerated shoots in <jats:italic>I. sonorae</jats:italic> explants. The induction of organogenic calli attained 100% efficiency. The highest percent shoot production was 87.5% with a mean of 9.17 shoots per explant on day 15, and the maximum length of 5.8 cm was observed on day 21. Regenerated shoots induced roots in B5 medium supplemented with 0.5–3.0 mg·L<jats:sup>−1</jats:sup> indole-3-butyric acid (IBA). The maximum rooting frequency observed in the medium containing 2.0 mg·L<jats:sup>−1</jats:sup> IBA was 83.3% which promoted long, thick roots on day 21. The plantlets with emerging roots grown at the culture facility attained 50% survival after acclimatization for 30 d. The account describes a simple and efficient protocol for in vitro plant regeneration, and this micropropagation procedure offers an alternative for preservation of this medicinal plant.</jats:p>

Direct in vitro organogenesis from leaf and internode of Coccinia cordifolia (L.) Cogn.

“…couldn't regenerate any buds at the only BAP treatment in the current study (Table 2). In contrast, BAP with auxin in combination proliferate shoots as exogenous auxin might activate the cytokinin signaling pathway for the production of endogenous cytokinins that results in shoots induction (Pemisova et al, 2009) and auxin also animated the cell division, cell elongation, reactivation of differentiated cells, additional vascular tissue development and rein the formation of a lateral organ (Arciniega-Carreón et al, 2017).…”